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Validated All-in-One™ qPCR Primer for MDK(NM_002391.4) Search again
Product ID:
HQP102205
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ARAP, MK, NEGF2
Gene Description:
midkine
Target Gene Accession:
NM_002391.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Requirement of chondroitin sulfate/dermatan sulfate recognition in midkine-dependent migration of macrophages
- In a blood vessel model, midkine induced stratification of endothelial cells and increased their proliferation and glycosaminoglycan synthesis. Increased proliferation of endothelium also occurred by coculture with smooth muscle cells and midkine.
- midkine binds to ALK and has a role in signal transduction for cell growth and survival
- Midkine is expressed in astrocytes during the early period of human brain ischemia.
- results suggest that the cell surface-expressed nucleolin serves as a low affinity receptor for midkine and could be implicated in its entry process
- Increased midkine expression is associated with superficial esophageal cancer
- Increased preoperative serum midkine in patients with esophageal squamous cell carcinoma is associated with poor survival.
- nuclear targeting growth factor midkine undergoes proteasomal degradation
- a G to T substitution at the 62nd site of intron 3 in the midkine gene enhances the expression of truncated midkine in colon cancer
- data showed that the midkine promoter activated a therapeutic gene in a wider range of human breast cancer than the c-erbB-2 promoter and suggest that MK promoter-mediated gene therapy is potentially more favorable in clinical settings
- MK participates in each of the two distinct phases of rheumatoid arthritis development, namely, migration of inflammatory leukocytes and osteoclast differentiation, and is a key molecule in pathogenesis
- Midkine promoter-based conditionally replicative adenovirus might be a promising new modality of gene therapy for malignant glioma.
- Increased midkine levels are associated with tumorigenesis in neurofibromatosis type 1
- MK may play roles, such as stimulation of endometriotic cell proliferation, in the development of endometriosis.
- reported that human MK exclusively localized to the nucleus and nucleolus in HepG2 cells by using GFP as a tracking molecule
- A survival molecule, midkine, was identified by cDNA array to be expressed only in drug-resistant neuroblastoma cells.
- Midkine likely plays a key role in human fetal adrenal glandcdevelopment.
- Resultsd suggested that truncated MK (tMK) has a greater ability of malignant transformation than full-length MK, and whether tMK is expressed or not will be useful information for improving cancer chemotherapy.
- localized exclusively to the nucleus and accumulated in the nucleolus in the three kinds of cancer cell lines
- Overexpression of midkine is associated with childhood B-precursor acute lymphoblastic leukemia
- Midkine, pleiotrophin (PTN), and their receptors syndecan-3 and receptor protein tyrosine phosphatase beta/zeta, were highly expressed in the striatum during developmen
- Antisense oligonucleotide targeting midkine suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced chick chorioallantoic membrane and in situ human HCC tissues.
- MK expression was increased along with tumor progression.
- Midkine expression was detected in the glomeruli, tubular epithelium and interstitium of kidneys from patients with diabetic nephropathy
- MK is involved in granulosa cell proliferation and estradiol production in developing follicles and may play a role as a local regulator in the human ovary.
- IL-6 and IL-8, and probably midkine and VEGF-A, appear to participate in the development of cancer-related cachexia in gastroesophageal malignancies.
- These data demonstrate a crucial role of MK-LRP1 signaling in anchorage-independent cell growth.
- Midkine has anti-apoptotic and cytoprotective role during cadmium toxicity in hepatocytes
- Midkine was expressed in choroid plexus of normal brain and released there; CSF MK levels were not high in patients with cerebral infarction but were increased in patients with meningitis
- MK is considered to mediate growth activity of odontogenic tumors and cell differentiation of odontogenic mixed tumors through molecular mechanisms similar to those involved in morphogenesis of the tooth.
- higher serum MK protein concentration was correlated with the presence of lymph node metastases and prognosis of endometrial carcinomas
- Midkine induces epithelial-mesenchymal transition through Notch2/Jak2-Stat3 signaling in human keratinocytes.
- increased expression in the prefrontal cortex of chronic alcoholics
- Increased serum midkine is associated with oral squamous cell carcinoma.
- midkine enhances soft-tissue sarcoma tumor growth
- The major finding of this study is a novel MK-triggered signaling mechanism implicated in migration and invasiveness of head and neck squamous cell carcinoma cells.
- midkine-mRNA expression was found in the majority of the neuroblastoma tissues. No correlation of MK status with survival, risk factors or disease stage was observed.
- Plasma MDK level is a prognostic factor for human neuroblastoma.
- Midkine prevents ventricular remodeling and improves long-term survival after myocardial infarction.