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Validated All-in-One™ qPCR Primer for LIF(NM_002309.4) Search again
Product ID:
HQP101796
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CDF, DIA, HILDA, MLPLI
Gene Description:
LIF interleukin 6 family cytokine
Target Gene Accession:
NM_002309.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq].
Gene References into function
- LIF stimulates proliferation of rat pituitary tumor cells in culture, inhibits secretion of prolactin and growth hormone and induces tyrosine phosphorylation of STAT3 in the cells.
- The entire structure of the LIF gene of 48 alleles in the Japanese population was sequenced.
- Oncostatin M and leukemia inhibitory factor regulate the growth of normal human breast epithelial cells
- LIF does not activate STAT3, ERK, or gp130 receptor in human N tera-2/D1 EC cells. The suppressor of cytokine signaling 1 (SOCS-1) is constitutively expressed, suggesting that LIF signal transduction is inhibited by elevated levels of SOCS-1 expression.
- experimental N-Myc overexpression results in down-regulation of leukemia inhibitory factor (LIF), a modulator of endothelial cell proliferation
- LIF stimulates (45)Ca release and enhances expression of receptor activator of NF-kappa B ligand (RANKL) and osteoprotegerin in neonatal mouse calvarial cultures, but has no effect on the expression of RANK.
- role for LIF in loss of autocrine prolactin suppression contributing to unrestrained prolactinomas prolactin secretion.
- LIF regulates cell survival of normal human endometrial stromal cells dose-dependently.
- Leukemia inhibitory factor (LIF), cardiotrophin-1, and oncostatin M share structural binding determinants in the immunoglobulin-like domain of LIF receptor
- LIF has a role in src family kinase-independent signal transduction and gene induction
- Leukemia inhibitory factor (LIF) increased overall expansion of human neural precursor cells grown in culture; Following LIF withdrawal, 200 genes showed significant decreases
- binding of ETS transcription factors to the ETS binding sites in the human LIF promoter is critical for its inducibility in response to T cell activators and play an important functional role within the endocrine-immune network.
- role in hepatocyte growth factor expression
- Decreased concentartion of LIF in infertile women do not seem to have a genetic basis due to the low frequency of LIF gene alterations.
- Constitutive expression of cytokines in brain induces changes in gene expression characteristic of chronic inflammation leading to either temporal weight reduction (CNTF) or severe cachexia (leukemia inhibitory factor).
- immunocytochemical staining and mRNA expression of LIF and its receptor are consistent with the concept that LIF might be involved in growth initiation of primordial follicles through its receptor
- At early human post-implantation stage, LIF is produced from decidua and chorionic villi and may exert its action on trophoblasts. Anembryonic pregnancy cannot be accounted for by defective expression of either LIF or LIF-Rbeta in most circumstances.
- Both IL-6 and LIF can be very efficiently induced by IL-1beta in articular chondrocytes in vitro, more so in osteosarthritis than in normal cells.
- LIFRbeta and the signaling subunit gp130 are expressed in hESCs and human LIF can induce STAT3 phosphorylation and nuclear translocation in hESCs.
- LCH represents a cytokine-driven condition partially mediated by TNF, IL-11, and LIF. These three cytokines are all osteoclastogenic, suggesting a pathogenetic pathway for the osteolytic lesions in LCH.
- These results indicated that LIF induced multi-lineage differentiation of adult stem-like cells in kidney via cadherin 16.
- role of leukemia inhibitory factor (LIF) and its receptors in human embryo implantation
- demonstrate using RNAi that Stat3 activation is necessary in the invasive phenotype of trophoblast cells and can be controlled via Leukemia Inhibitory Factor (LIF)
- different members of the TGF-beta superfamily may also contribute in the reproductive process by enhancing endometrial proimplantatory LIF secretion and reducing proinflammatory IL-6 release by endometrial epithelium
- LIF plays an essential role in the preimplantation embryo development and the blastocyst implantation and its gene mutations in women contribute to the implantation failure and subsequent infertility.
- Leukemia inhibitory factor concentration in follicular fluid is not a useful marker for the prediction of number and quality of embryos, or implantation and pregnancy rates.
- Data show that increased leukemia inhibitory factor secretion enhances airway reactivity and intracellular calcium signaling.
- results suggest that the LIF gene mutations affect fertility
- Since we demonstrated that not only SDF-1, but also HGF and LIF are upregulated in damaged tissues, we postulate that CXCR4+ c-Met+ LIF-R+ TCSC could be mobilized from the BM into the PB, where they play a role in tissue repair/regeneration.
- Down-regulation of PRB in the endometrium is concomitant with the presence of glycodelin in the endometrium, suggesting interaction.
- Suppressor of cytokine signaling-1 may prevent leukemia inhibitory factor signaling in human embryonic stem cells.
- There is no receptivity defect with regard to LIF and IL-11 secretions by eutopic endometrium in infertile women with endometriosis.
- Ciliary neurotrophic factor/LIF signaling pathway acts via regulation of nitric oxide production to modulate developmental programmed cell death of postmitotic rod precursor cells.
- The regulation of LIF and its receptor (LIFR) expression in pancreatic carcinoma cells were studied.
- provides a structural template to understand the formation and orientation of the high-affinity signaling complex between LIF, LIF receptor, and gp130
- interleukin-8 (CXCL-8) is induced by tumor necrosis factor-alpha and leukemia inhibitory factor in pancreatic carcinoma cells
- Endometrial integrin beta3 and LIF expressions in the peri-implantation phase were significantly lower in stimulated cycles (including both moderate and high responders) compared to natural controls.
- LIF and IL6 skew monocyte differentiation into tumor-associated macrophage (TAM)-like cells by enabling monocytes to consume monocyte-CSF. Depletion of LIF, IL-6, and M-CSF in ovarian cancer ascites suppressed TAM-like cell induction
- the LIF gene mutation, the heterozygote G to A transition on the position 3400, affects fertility but the infertility treatment can succeed.
- Increased expression of some IL-6 cytokine family members (oncostatin M, gp130, CT-1, LIF) in cutaneous inflammation might contribute to the promotion of hair loss.
- Increased LIF expression in peri-infarcted regions and sequestration from the peripheral circulation in acute stroke patients are characteristic of the pathobiological response to ischaemia and tissue damage.
- Present predominantly in glandular epithelium.
- higher levels found in the placentas of pre-eclamptic compared with normotensive women
- Immunohistochemical labeling of LIF in the fallopian tube was found to be increased in ectopic pregnancies compared to non-pregnant and healthy pregnant controls
- The antiphospolipid antibodies (aPLs) are elevated in infertile women with LIF gene mutations.
- secretion of OSM and LIF by both epithelial and stromal (paracrine manner) cells seems to promote tumor growth in human breast carcinoma
- LIF can regulate extravillous trophoblast invasion, suggesting an important role in early placental development.
- Overexpression may be a potential molecular marker for predicting poor prognosis in patients with epithelial ovarian carcinoma.
- Results describe the role of leukemia inhibitory factor in human mesenchymal stem cell-mediated immunosuppression.
- A potential role for LIF in the melanoma-induced bone metastasis possibly through the stimulation of osteoclastogenesis is suggested.
- In silico models predicted that five of the up-regulated miRNAs targeted leukemia inhibitory factor (LIF) expression