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Validated All-in-One™ qPCR Primer for LEPR(NM_002303.5) Search again
Product ID:
HQP101782
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD295, LEP-R, LEPRD, OB-R, OBR
Gene Description:
leptin receptor
Target Gene Accession:
NM_002303.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Leptin (LEP; MIM 164160), an adipocyte-specific hormone that regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure, acts through the leptin receptor (LEPR), a single-transmembrane-domain receptor of the cytokine receptor family.[supplied by OMIM].
Gene References into function
- A human liver cell line expresses the leptin receptor long form Ob-Rb in sufficient quantity to respond to leptin signalling in terms of STAT activation.
- Cultured human articular chondrocytes express functional leptin receptor Ob-R, a receptor present on chondrocytes in native human cartilage.
- Sleep apnea syndrome is associated with plasma soluble leptin receptor and insulin resistance independently of body mass index.
- leptin receptors are expressed in breast cancer; data suggests they may be implicated in mammary cell proliferation and in breast cancer pathogenesis
- the levels of mRNA for the long and total forms of leptin receptors were suppressed in association with the severity of endometriosis
- results suggest that DNA sequence variations in the leptin receptor gene could affect substrate oxidation
- presence of the long leptin receptor isoform in the absorptive cells of the small intestine, suggesting that leptin could have a physiological role in the regulation of nutrient absorption
- confirmation of expression in primary osteoblasts
- relationship of soluble leptin receptor with the degree of adiposity suggests that high SLR levels may enhance leptin action in lean subjects more than in obese subjects
- regulation of circulating levels by gender, adiposity, sex steroids and leptin
- Leptin receptor (Ob-R) expression is induced in peripheral blood mononuclear cells by in vitro activation and in vivo in HIV-infected patients, consistent with the suggested role of leptin in modulating the immune response.
- during weight loss leptin levels decreased, whereas soluble leptin receptor levels and the receptor bound fraction of leptin increased
- modifications in ciruclating levels across the eating disorder spectrum
- Serum leptin and leptin binding activity in children and adolescents with hypothalamic dysfunction.
- cloning, expression and purification of a recombinant leptin-binding domain
- regulated shedding of the ectodomain of membrane-spanning leptin receptors may represent a novel mechanism of modulating leptin's biological activity
- transforming growth factor beta effects an increase in formation
- high levels of soluble leptin receptor in emaciation may reflect an up-regulation to suppress leptin action during energy deficiency and with free leptin index is a tool to investigate the leptin axis during growth and sexual maturation
- term pregnancy human umbilical cord and fetal membranes co-express leptin and its receptor genes
- Significantly increased in anorexia nervosa patients. Increase unaffected by partial refeeding. Possible etiological role of increased soluble leptin receptor levels in anorexia nervosa by affecting leptin central and/or peripherial effects.
- Human natural killer cell lines express leptin receptors.
- the tumor clones in childhood acute lymphoblastic leukemia do not express the leptin receptor
- higher leptin levels for those with a leptin receptor Lys109Arg or Gln223Arg mutation may imply that these subjects have a modified functional leptin receptor
- Skillful mechanism where a change in the serum SLEPR level regulates, in part, the biological activity of leptin in the circulation.
- in living cells approximately 60% of the leptin receptor exists as constitutive dimers at physiological expression levels in the absence of leptin
- Serum adiponectin and soluble leptin receptor levels might be influenced by common regulatory factors and challenge the notion that cortisol may have a direct inhibitory effect on adiponectin in humans.
- low serum leptin, high serum TGF-beta1 and sOb-R levels, and elevated urine leptin concentrations were observed at the onset of minimal change nephrotic syndrome
- Possibility that soluble leptin receptor is a minor component of leptin binding capacity in the plasma of pregnant women.
- role of the soluble leptin receptor (sOB-R) in maintaining serum leptin levels in nephrotic patients
- Ob-R gene may serve as a novel modifier gene for hypercholesterolemia in Japanese men.
- Hints for a peripheral role of leptin in the male genital tract, possibly, by an interaction between leptin and spermatozoa via sperm leptin receptors.
- Soluble leptin receptor levels are not altered by the cyclic hormone status in the menstrual cycle.
- LEPR single nucleotide polymorphisms contribute to variation in obesity phenotypes.
- The leptin receptor is aberrantly expressed in bladder cancer tissue and is possibly involved in the carcinogenesis of bladder cancer.
- Lower expression levels of leptin receptor short form (Ob-Ra) were observed in most endometrial cancer tissues, especially in the poorly differentiated ones.
- Expression of leptin receptor in arcuate nucleus resulted in normalization of estrous cycle length, increased ovarian follicular development, and decreased serum progesterone levels.
- plasma SLR levels are independently regulated by many different physiological and pathophysiological conditions and SLR may modulate the actions of leptin
- low levels of expression of leptin receptors at the cell surface results from partial retention in the biosynthetic pathway, coupled to constitutive removal from the plasma membrane via ligand-independent, constitutive endocytosis
- Secretory endometrium is target for leptin, and oocytes and preimplantation embryos possess OB-R mRNA. Leptin may be necessary for embryonic development. Leptin mRNA expressed at blastocysts stage. Possible function in blastocyst-endometrial dialogue.
- Leptin receptor polymorphism (Gln223 Arg) has association with peak bone mass in young women.
- Association of Q223R polymorphism of LEPR gene with insulin resistance. Q223R polymorphism was independently associated with insulin sensitivity index and glucose clearance.
- transactivation occurs through an indirect interaction between erbB2 and prolactin or leptin receptors
- Interaction between the LEP A19G and LEPR K109R polymorphisms on the change in fasting insulin.
- leptin and leptin receptor may have a promoting effect on the carcinogenesis and metastasis of breast cancer
- Increase in levels of soluble OBR and decrease in free leptin index in adolescent girls with anorexia nervosa. Weight recovery is associated with decrease in soluble OBR and increase in leptin.
- serum soluble leptin receptor level is negatively correlated with homeostasis model assessment-insulin resistance index
- Several isoforms of the OB-R, including the signal transducing full-length receptor, are expressed in most human pituitary adenomas
- found similar leptin and OB-Rs plasma levels between patients suffering from remitting-relapsing multiple sclerosis and controls
- poor glycemic control in diabetes may lead to increased serum levels of soluble OB-R.
- Low soluble leptin receptor concentration is associated with a 6.3-fold increased risk of preeclampsia
- Leptin receptor SNPs do not appear to be important factors in the regulation of body weight in young, pre-menopausal women or have any significant association with anorexia nervosa.
- leptin receptor (OB-Rb) mRNA is expressed in cultured commercially available human osteoblasts
- This study provides evidence to suggest cross down-regulation of leptin and insulin receptors at both receptor and downstream signalling levels.
- Effect pf genetic and environmental factors on levels in female twins in puberty.
- relationship between the leptin receptor (OB-R) polymorphism and conversion from impaired glucose tolerance (IGT) to type 2 diabetes was not found but insertion allele was associated with reduction in weight, body mass index and waist circumference
- The IVS20-11delT variant of the NPPS gene and the A861G variant of the leptin receptor gene are associated with more extensive OPLL, but not with the frequency with which it occurs.
- the activated LR complex is formed by leptin-induced clustering of two preformed dimers using critical cysteine residues in its fibronectin type III subdomains
- platelets are a major source of leptin receptor in the circulation; leptin-responsive individuals may have a higher risk for obesity-associated thrombosis than non-responsive individuals
- Human neutrophils express leptin surface receptors under in vitro and in vivo conditions; leptin surface receptors are readily detected on neutrophils infiltrating appendicitis and ulcerative colitis tissues.
- The leptin receptor is present in human endothelial progenitor cells & leptin may affect their function, both in physiological & in hyperleptinemic conditions.
- Polymorphisms in the leptin receptor (LEPR)are positively associated with body mass index, and putatively associated with obesity and type 2 diabetes mellitus.
- Leptin and the molar excess of leptin receptor are better biomarkers of nutritional status than IGF-I in protein malnutrition children during nutritional recovery.
- Staining for leptin and the long form of its receptor was identified in oocytes of follicles from primordial stages onward, and for leptin only in granulosa cells of a minority of the samples
- Increased expression of leptin receptor is associated with breast cancer progression
- a conserved cluster of residues Leu370, Ala407, Tyr409, His417, and His418 forms the center of binding site III of the LR
- Association of leptin receptor variability with inflammatory traits supports the hypothesis that leptin may play a role in atherogenesis
- The high expression levels of leptin receptors are biological markers of a more differentiated phenotype and isoform OBR-S is an independent prognostic factor.
- Longitudinal changes in hip or forearm body mass index in elderly men were not associated with LEPR genotypes
- The leptin receptor with the conserved Y985 and Y1077 motifs in the cytosolic domain is binding of CIS (cytokine-inducible SH2 protein).
- There is a strong association of the LEPR p.Q223R gene polymorphism with obesity in Brazil
- Obesity-related leptin receptor polymorphisms are not associated with gallstones disease.
- The near absence of OB-Rb (long isoform) mRNA and the similarly decreased SOCS3 expression in obese adipose tissue may reflect a defective leptin signaling pathway.
- Overexpression of leptin and expression of OB-R may play a positive role in the process of progression in gastric cancer.
- Q223R polymorphisms in the LEPr gene was significantly associated with obesity.
- Demonstrates for the first time increased leptin receptor expression in liver tissue and its relationship with overexpression of TGF-beta1 and the degree of hepatic fibrosis.
- I allele of the 3' UTR of the leptin receptor signaled a reduced risk of type II diabetes development in Mexicans
- LEPR A223G polymorphism may individually modify the risk of severe pre-eclampsia
- An analysis of frequency distribution of the Gln233Arg polymorphism in the leptin receptor gene in children with acute lymphoblastic or acute myeloblastic leukemia showed lack of differences between the patients and controls.
- LEPR gene polymorphisms do not associate with obesity.
- Leptin and TGF-beta1 synergistically augmented activation of signalling components of mitogen-activated protein kinase (MAPK), STAT3 and Smad but did not modulate the expression of LEPR-B.
- Allelic variants are specifically associated with distinctly different eating patterns, namely extreme snacking behavior or excessive portion size.
- findings show that dysfunction of spermatogenesis is associated with an increase in leptin and leptin receptor expression in the testis
- Gln223Arg associated with risk of vertebral fracture (overall OR = 1.76; OR in smokers = 2.31; p = 0.0004), in addition to BMD of the femoral neck and total hip (p = 0.036 and 0.008, respectively).
- genetic variability in the leptin receptor gene may predispose some individuals with schizophrenia to excessive weight gain from increased exposure to olanzapine
- The increased leptin levels in synovial fluid point toward a local effect of leptin in articular cartilage, while the observed intrajoint differences of leptin and Ob-Rb mRNA expression may be related to the grade of cartilage destruction
- Positive correlation is observed between leptin (Ob) receptor and hypoxia-inducible factor 1 alpha subunit (HIF-1alpha)expression in endometrial cancer.
- Statistically significant coexpression of leptin and its receptor are involved in colorectal cancer progression related to the abundance of hypoxia-inducible factor (HIF)-1 alpha in tissue specimens from patients undergoing curative resection.
- Leptin induces CRP expression in HCAECs via activation of the leptin receptor, increased ROS production, and phosphorylation of ERK1/2
- leptin serum levels & soluble leptin receptors levels were studied in Prader-Willi & Down syndromes; data suggest that whereas PWS patients have a leptin assessment corresponding to degree of obesity, DS subjects may have a defect in secretion of leptin
- support a possible action of leptin on the cardiovascular system by demonstrating expression of the leptin receptor in different types of vascular lesions
- the phosphorylation of Tyr(1077) on LepRb during receptor activation, substantiate the hypothalamic regulation of STAT5 and S6 by leptin, and define the alternate LepRb signaling pathways
- mammalian cell-expressed sOb-R (leptin receptor) fragment (residues 22-839 of the extracellular domain) bound leptin with a dissociation constant of 1.8 nM. This binding was inhibited by ConA or wheatgerm agglutinin
- study suggests an association between serum leptin concentration and tumor progression; LEPR-109 polymorphism in premenopausal women appears to be associated with obesity and tumor progression.
- Several polymorphisms of leptin gene and its receptor have been described as potential developmental factors of pregnancy complications such as diabetes mellitus or pregnancy induced hypertension.
- There were no associations of leptin receptor genotypes or haplotypes and adenoma risk
- Data demonstrate that IRS4 interacts with the LR. This recruitment is leptin dependent and requires phosphorylation of the Y1077 motif of the LR.
- Arg allel or LEPR seems linked to a higher risk of obesity and higher lipid levels in obstructive sleep apnea syndrome patients
- single nucleotide polymorphisnms on gene involved with glucose metabolism and obesity may be associated with increased susceptibility to spina bifida
- study suggests the LEPR Lys109Arg polymorphism is one of the genetic determinants of the peak bone mass in Korean women
- the genotype and allele frequencies of the LEPR p.Q223R variant were significantly different between the normal weight and obese groups
- Statistical significance was noted expression of leptin receptor.
- Ob-R expression was positively correlated with tumour size may point to a potential role of leptin as a growth factor and of Ob-R as a new prognostic factor.
- Genetic variation is associated with ypte 2 diabetes in a Northern Chinese population.
- Mean serum leptin level and body mass index in the non-alcoholic fatty liver disease (NAFLD) group were significantly higher than in the controls, but mean soluble leptin receptor level was lower in the NAFLD group when compared to the controls.
- leptin may act as a growth-promoting signal during fetal development, and suggests a possible role for the LEPR in explaining the inverse relationship between birth weight and the development of metabolic diseases in adulthood.
- Ghrelin, leptin, and soluble leptin receptor were associated with number of metabolic syndrome components cross-sectionally, consistent with the hypothesis that these adipokines may have a central role in cardiometabolic risk.
- Our results point to an influence of the Lys656Asn polymorphism in the LEPR gene on the response to different diets.
- Expression levels of leptin receptor-coding gene showed significant differences between anorexia nervosa (AN)-binge/purge type and AN-restricting (R) type patients and between AN-R and control subjects.
- LEPR+5193G>A, +7187A>C, +27265G>A, +35861T>C, and +52289A>G showed strongly significant association with body mass index 9606
- leptin/Ob-R correlate with angiogenesis and tumor progression in patients with HCC and that an anti-leptin antibody exerts an angiostatic activity in HCC.
- Influence of Lys656Asn polymorphism in the leptin receptor gene on adipocytokines and insulin resistance in naive patients with diabetes mellitus type 2.
- study reports expression of the leptin receptor isoforms in osteocytes isolated from biopsies in brain injury-related heterotopic ossification
- Leptin gene and leptin receptor gene polymorphisms are associated with sweet preference and obesity.
- leptin-receptor play a role in the development of obesity through leptin resistance and blunted leptin-mediated sympathetic nerve activity.
- Leptin receptor is elevated in carotid plaques from neurologically symptomatic patients and positively correlated with augmented macrophage density.
- a role for leptin receptor in melanoma
- there is a gender dimorphism in skeletal muscle leptin receptors expression, which can be partly explained by the influence of testosterone
- There was a trend found among the majority of homozygous A and G variants in LEP -2548 G/A and LEPR 668 A/G in over-weight and obese individuals in comparison to normal-weight subjects (CG).
- Leptin receptor did not reveal a definite difference between benign prostatic hyperplasia and prostate cancer
- Leptin may play a role in the process of intervertebral disc degeneration by contributing to the formation of cell cluster and proliferating fibrocartilaginous tissue.
- Q223R allele of the leptin receptor is unlikely to play a significant role in regulation of human adiposity.
- leptin receptor expression in tumors is involved in adaptive immune response in sporadic colon and rectal tumors likely via MSI-H phenotype orientation.
- leptin increases the expression of several cytokines in normal and tumoral colonocytes by targeting leptin receptor.
- Gln223Arg polymorphism in the extracellular domain of the LEPR receptor gene is associated with a modestly increased risk of premenopausal breast cancer in Nigerian women
- Polymorphisms in the IL6, LEPR, TNF and ADIPOQ genes are not associated with prostate cancer, while allelic variants in the LEP gene are related to prostate cancer risk, supporting a role for leptin in prostate carcinogenesis
- LEPR Q223R polymorphism may be associated with obesity in women with a psychotic disorder treated with atypical antipsychotic drugs.
- oncogenic transformation of nonmalignant mammary cell lines increased expression of leptin receptor
- Polymorphisms of the LEPR and GHRL seem unlikely to be associated with asthma or BMI in our sample. The results of haplotype analysis for the LEPR gene may suggest a protective role of the GG haplotype against asthma.