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Validated All-in-One™ qPCR Primer for ITGAV(NM_002210.4) Search again
Product ID:
HQP101186
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD51, MSK8, VNRA, VTNR
Gene Description:
integrin subunit alpha V
Target Gene Accession:
NM_002210.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Gene References into function
- Processing of integrin alpha(v) subunit by membrane type 1 matrix metalloproteinase stimulates migration of breast carcinoma cells on vitronectin
- Acute hyperglycaemia induces an up-regulation of seven major genes, four of which were not previously reported in the literature. Northern blot analyses, performed on these 4 genes, confirm macroarrays results for alphav, beta4, c-myc, and MUC18.
- Review. Alpha(v)beta3 integrins are involved in vascular repair processes. The challenge is to develop a therapeutic agent that will prove effective in reducing restenosis in humans following percutaneous coronary intervention (PCI).
- They analyzed the expression of these cell surface receptors in nine ovarian cancer cell lines and also in the primary human ovarian surface epithelial cell line (HOSE).
- Unique ability of integrin alpha(v)beta 3 to support tumor cell arrest under dynamic flow conditions (Integrin alpha-v beta-3)
- Del1 mediates vascular smooth muscle cell adhesion, migration, and proliferation through interaction with integrin alpha(v)beta(3).
- Factor XIII mediates adhesion of platelets to endothelial cells through alpha(v)beta(3) and glycoprotein IIb/IIIa integrins.
- existence of an alpha V integrin/vitronectin connection in hepatocellular carcinoma and suggest that this connection may be an adverse prognostic factor.
- Alpha(v)beta(3) integrin engagement enhances cell invasiveness in human multiple myeloma.
- integrin alphaVbeta3 expression is reduced when ROR alpha is activated in prostate cancer cells
- Integrin alphavbeta3. Expression of integrin alpha v beta 3 promotes the metastatic phenotype in human melanoma by supporting specific adhesive, invasive and migratory properties of the tumor cells
- alpha v beta 3 integrin signaling through Shc recruitment in response to mechanical stimulation in human osteoblasts
- phosphoinositide 3-kinase C2alpha was found to be differentially regulated by alpha(v)beta(3) engagement
- promotion of integrin alpha Vbeta 3-dependent endothelial cell adhesion by PGE2
- identification of a new alphavbeta5 integrin-interacting motif that is highly conserved in the fas-1 domains of many proteins suggesting that fas-1 domain-containing proteins may perform their biological functions by interacting with integrins
- induced alpha(v)beta(8) integrin expression mediated Fas-stimulated migration
- identification of functional segments within the beta2I-domain
- Data show clearly that integrin alpha(v)beta(3) interacts with the latency-associated peptide (LAPbeta1 and 3) RGD motif.
- role in mediating pro-angiogenic activity of CYR61
- An autocrine loop of the integrin alpha(V)beta(3)/CD47 receptor complex and thrombospondin-1 is identified as the molecular coupling device between mechanical loading and apoptosis.
- in patients with endometriosis a significant negative correlation was observed between luminal expression of eNOS and alpha(v)beta(3) integrin and between glandular expression of eNOS and luminal expression of alpha(v)beta(3) integrin
- thrombin interacts with alphavbeta3 as demonstrated by direct binding of alphavbeta3 protein to immobilized thrombin.
- Overexpression of integrin alphaV promotes human osteosarcoma cell populated collagen lattice contraction and cell movement.
- activation state of alphaVbeta3 integrin is an important regulator of the duration of insulin-like growth factor I receptor phosphorylation and this regulation is mediated through changes in the subcellular localization of SHP-2
- Data show a novel mechanism by which ECM regulates membrane-type 1 matrix metalloproteinase (MT1-MMP) association with beta1 or alphavbeta3 integrins, thus modulating its internalization, activity, and function on human endothelial cells.
- integrin alpha5beta1-mediated control of the levels of integrin alphaVbeta3 is important for the distribution of focal contacts
- has a role in the early phase of liver metastasis
- thymidine phosphorylase and 2-deoxyribose-induced focal adhesion kinase phosphorylation was blocked by the antibodies to integrins alpha 5 beta 1 and alpha v beta 3, directly linking their migration and signaling components
- role in regulating cell proliferation through integrin-linked kinase (ILK) in ovarian cancer cells
- decreased expression of endometrial integrin alphavbeta3 suggests that functional, but not morphological, endometrial defect may be one of the causes for the patients with unexplained infertility and recurrent IVF-ET failures
- Alphavbeta3 integrin expression and pinopode formation in human endometrium are processes closely related to endometrial maturation; unlikely as targets for contraceptive approaches or fertility-promoting strategies
- tumstatin binds to alpha v beta 3 integrin in a vitronectin/fibronectin/RGD cyclic peptide independent manner
- Data suggest that this alphavbeta3 binding to alpha5-laminins is involved in the regulation of cellular responses to growth factors known to be involved in epithelial and endothelial development.
- signaling through JAM-1 and alphavbeta3 is necessary for bFGF-induced angiogenesis.
- Data provide the first evidence that alphavbeta3 integrin can generate apoptosis-stimulating signals.
- alpha 5 beta 1 and alpha v beta 3 are both important but cell-specific fibrillin-1 receptors
- integrin alphavbeta3 cooperates with metalloproteinase MMP-9 in regulating migration of metastatic breast cancer cells
- These data provide evidence for a functional role for integrin alphavbeta8-mediated activation of transforming growth factor-beta in control of human airway epithelial proliferation in vivo.
- AlphaV- and beta1-integrin subunits are commonly expressed in malignant effusions from ovarian carcinoma patients.
- integrin alpha(v)beta3 has an important role in bone metabolism and angiogenesis, and in controlling growth of metastatic prostate cancer cells in the bone
- Disulfide bonding patterns of the integrin alpha V chain are described; the alpha chain displays differences in the disulfide patterns of those bonds near their C-terminal regions linking the heavy and light chains.
- alpha3beta1, alpha4beta1 and alphaVbeta1 integrins may play an important role in the implantation process
- alphav subunit cleavage is essential for integrin function and has a considerable impact on integrin-dependent events, especially those leading to cell migration
- alpha(v)beta(1) supports fetal beta-cell migration and could play an important role in early motile processes required for islet neogenesis
- CD47-alpha(v)beta(3) interactions that lead to integrin clustering are detergent resistant and do not require cholesterol or the transmembrane region of CD47
- alphavbeta3 integrin has a role in regulating proliferation and apoptosis of hepatic stellate cells
- Role of alpha4, alpha5, and alphav integrin receptors--which are central to mediating interactions with these domains of FN--in regulating ssquamous cell carcinoma migration.
- integrin alpha(v)beta(6) is an RGD-dependent receptor for Coxsackievirus A9 and may be important in natural Coxsackievirus A9 infections
- alpha-v integrin signals through JAB1 (Jun activation binding protein 1) to prolong MMP1 production
- secondary structure analysis of integrin alphaVbeta3
- CEACAM1 and alpha(v)beta(3) integrin are functionally interconnected with respect to the invasive growth of melanomas
- Integrin alphav controls melanoma cell survival in 3D-collagen through a pathway involving p53 regulation of MEK1 signaling.
- alphav integrins and MMP2 have roles in migration of human ovarian adenocarcinoma cells through endothelial extracellular matrix
- The Integrin alphaV receptor is members of the group of the cellular adhesion molecules (CAM), are heterodimeric transmembrane glycoprotein receptors involved in processes such as cell-cell and cell-matrix adhesion, cell migration and signaling.
- BMP-2 upregulates the expression of alphavbeta integrins, and these integrins, in turn, play a critical role in BMP-2 function in osteoblasts
- Pyk2 is a phosphorylated beta(3) binding partner, providing a potential structural and signaling platform to achieve alpha(V)beta(3) -mediated remodeling of the actin cytoskele
- We conclude that the ectodomain of alphaVbeta3 manifests a bent conformation that is capable of stably binding a physiological ligand in solution.
- We conclude that integrin alphavbeta6-dependent MMP-2 and -9 up-regulation is an important feature of increased migration in hypoxic human keratinocytes.
- Epidermal growth factor receptor and alphavbeta3 work together as coreceptors for Human cytomegalovirus entry and signaling.
- urokinase receptor-derived SRSRY peptide regulates cross-talk between fMLP and vitronectin receptors
- new pair of adhesion molecules, Thy-1 and alphavbeta3, inditified that mediate the interaction of melanoma cells and activated endothelial cells
- IGROV1 cells were shown to adhere rapidly on the HUVECs monolayer; adhesion was inhibited by anti-alphav integrin and anti-Vn blocking antibodies, but not by anti-beta1 integrin antibodies
- Gal3ST-2 is involved in tumor metastasis process by regulation of adhesion ability to selectins and expression of integrin alphaV but not beta3
- melanoma cells cross-present, in an alpha v beta3-dependent manner, an antigen derived from secreted matrix metalloproteinase-2 (MMP-2) to human leukocyte antigen A*0201-restricted T cells
- integrin alphavbeta6, alphavbeta3, alphavbeta5, alpha5beta1 and alpha9beta1 binding to osteopontin is controlled by specific structural motifs that are recognized by extracellular proteases
- Alphavbeta6 integrin down-regulates MMP-13 expression in oral squamous cell carcinoma cells.
- Increased transcripts of activators of TGFB1 (Itgav and Thbs1), and genes that are activated by TGFB1 (Spp1, Timp1, and Tnc) occurs during acute lung injury
- Data show that alpha(v)beta3/Tat interaction triggers the activation of NF-kappaB in endothelial cells in a focal adhesion kinase-, RhoA- and pp60src-dependent manner, and this activation is required for motogenic activity of Tat in endothelial cells.
- alphavbeta3 integrin is transiently up-regulated (and activated) in graft arteriopathy
- the RGD-dependent interaction between the P2Y(2)R and alpha(v) integrins is necessary for the P2Y(2)R to activate G(o) and to initiate G(o)-mediated signaling events leading to chemotaxis
- Expression of integrins alphavbeta1, alphavbeta3, and alphavbeta5 in cerebral arteriovenous malformations and cavernous malformations. (Integrins alphavbeta1, alphavbeta3, and alphavbeta5)
- the signal interaction between IGF-1R and alpha(v)beta3 integrin plays an important role in promoting the development and progression of cervical cancer
- cardiotoxin A5 binds to alpha(v)beta3 integrin and inhibits bone resorption
- These results indicate that the alpha2beta1 and alphaVbeta1 integrin signaling pathway may be critical components of neurodegeneration in Alzheimer's disease.
- thymosin beta10, but not thymosin beta4, have direct inhibitive effects on endothelial migration and tube formation that might be mediated via downregulation of VEGF, VEGFR-1 and integrin alphaV in HCAECs.
- While tolerogenic DCs are not induced via alphavbeta5, coligation of CR3 and alphavbeta5 maintains the DC's tolerogenic profile.
- Findings suggest that in melanoma cells the increase in MMP-2 activity, CD44 surface expression, and cell migration is integrin alpha V-dependent.
- The effects of the EBV nuclear antigen 3C on the activities of Nm23-H1 via GATA-1 and Sp1 are reported.
- Alphavbeta5 integrin may play a role in the adhesion and migration of VSMCs during the pathogenesis of atherosclerosis.
- upregulated expression of alphavbeta5 contributes to autocrine TGF-beta signaling in localized scleroderma fibroblasts
- periostin has an active role in the epithelial-mesenchymal transformation and metastasis that requires cross-talk between integrin and EGFR signaling pathways
- integrin alpha3beta1 is a receptor for the alpha3NC1 domain and transdominantly inhibits integrin alphavbeta3 activation
- binding of resveratrol to integrin alphaVbeta3, principally to the beta3 monomer, is essential for transduction of the stilbene signal into p53-dependent apoptosis of breast cancer cells.
- PET Molecular imaging of alpha(v)beta3 expression with [18F]Galacto-RGD in humans correlates with alpha(v)beta3 expression as determined by immunohistochemistry.
- S 36578-2 is highly selective for alpha v beta3 and alpha v beta5 integrins and induces detachment, caspase-8 activation, and apoptosis in human umbilical endothelial cells
- These findings elucidate the function and complex regulation of integrin-mediated activation of TGF-beta within the epithelial-mesenchymal trophic unit (EMTU).[integrin alphavbt=eta6, integrin alphavbeta8]
- integrin binding loop of Foot-and-mouth disease virus has most likely evolved for binding to alphavbeta6 with a higher affinity than to alphavbeta3 and alphavbeta8
- The transition from a locally invasive phenotype to a metastatic phenotype may be primed by the elevated expression of alpha(v)beta(3) receptors in prostate cancer cells.
- identifies integrin alphaVbeta6 as a novel cellular receptor for fibrillin-1 with a K(d) of approximately 0.45 mum
- type I collagen induction of MT1-MMP-mediated MMP-2 activation is repressed by alphaVbeta3 integrin in human breast cancer cells
- Mn(2+) promoted convergent changes in integrin alphavbeta6 conformation and Fb structure through activation of ERK/MAPK and MMP-9
- integrins alpha5beta1 and alphaV are inhibited by lebectin and lebecetin snake venom lectins
- Adhesion of breast cancer cells through the VLA integrins alpha2beta1 and alpha5beta1 was significantly reduced by an apoptosis-inducing natural triterpenoid, dehydrothyrsiferol (DT), when studied on low amounts of extracellular matrix.
- Prostate cancer cells expressing fully functional but not dysregulated alphavbeta3 integrin are able to control their own adherence and migration to bone matrix, functions that facilitate tumor growth and control bone lesion development.
- Necl-5 has a critical role in integrin alphavbeta3 clustering and focal complex formation
- Endothelial cell integrin alphaV beta3 is involved in sickle cell red blood cell adhesion.
- Results suggest that CTGF plays a crucial role in migratory/invasive processes in human breast cancer by a mechanism involving activation of the integrin-alphavbeta3-ERK1/2-S100A4 pathway.
- The rs3738919-C allele of the ITGAV gene is associated with rheumatoid arthritis in the European Caucasian population, suggesting ITGAV as a new minor susceptibility gene for rheumatoid arthritis.
- establishes alphavbeta3 integrin as one key component of the transendothelial migration process of tumor cells, and as a potential target for anti-metastatic therapy
- c-Src controls functional association between integrin alphav-beta3 and VEGFR-2 via integrin beta3 phosphorylation.
- macrophage migration inhibitory factor directly up-regulates alphavbeta3 integrin and VEGF expression in human endometrial Ishikawa cells
- These findings provide evidence that mutations in fibronectin, induce anoikis in human squamous cell carcinoma cells by modulating integrin alpha v-mediated phosphorylation of FAK and ERK.
- the studies demonstrate that Vbeta3 is a cellular receptor mediating both the cell adhesion and entry of KSHV into target cells through binding the virion-associated gB(RGD).
- Report observed that alphav-beta3 integrins and Focal Adhesion Protein-Tyrosine Kinases co-localize and that fibrillin filament attachment sites to endothelial cells merge with these molecules.
- anti-tumor and anti-metastatic activities of CNTO 95 in breast cancer models and provide insight into the cellular and molecular mechanisms mediating its inhibitory effects on metastasis.
- Signal transduction pathways underlying the enhanced cell migration reveal that the IGF-I-IGFBP-VN complex stimulates a transient activation of the ERK/MAPK signaling pathway and a sustained activation of the phosphatidylinositide 3-kinase/AKT pathway.
- uPAR is required to activate alphavbeta3 integrin in podocytes, promoting cell motility and activation of the small GTPases Cdc42 and Rac1
- Alpha v beta 5, alpha v beta 5 and their ligands Del-1 and L1 play an important role in the process of tumor cells moving from the original place.
- TGF-beta1 acts through PI3K/Akt, which in turn activates IKKalpha/beta and NF-kappaB, resulting in the activations of alphavbeta3 integrins and contributing the migration of chondrosarcoma cells
- We used suppression subtractive hybridization (SSH) to study molecular signature of melanoma progression, by showing characteristics of early neoplastic lesions including expression of KISS1, lack of alphavbeta3-integrin and low levels of RHOC.
- Integrin alpha v beta 3 plays a critical role in the S1P-stimulated chemotactic response of ECs
- The shear-induced G(2)/M arrest and corresponding changes in G(2)/M regulatory protein expression and activity were mediated by alpha(v)beta(3) and beta(1) integrins through bone morphogenetic protein receptor type IA-specific Smad1 and Smad5.
- Mn(2+) simultaneously modulates the thiol isomerase activity of protein disulfide isomerase that is bound to alpha(V)beta(3) and induces its transition to the ligand-competent state, suggesting an alternative mechanism of integrin regulation
- Data show that in preeclamptic pregnancies, histamine does not stimulate expression of integrin alphaVbeta3, as it does in uncomplicated pergnancies.
- These results suggest that activation of TGF-beta1 by integrin alphavbeta6 contributes to pathological changes and may impair endothelial cell functions in tissues that are chronically infected with cytomegalovirus.
- Infertile women with isolated polycystic ovaries are deficient in endometrial expression of osteopontin but not alphavbeta3 integrin during the implantation window.
- results demonstrate that TGFbeta1 induces alphavbeta3 integrin expression via a beta3 integrin-, c-Src-, and p38 MAPK-dependent pathway
- ROCK-dependent actomyosin-driven contractility is a critical determinant for the regulation of the interaction between Necl-5 and integrin alpha(V)beta(3).
- significant difference in the expression of the beta(3) integrin subunit between primary glioblastomas compared with low-grade gliomas
- integrin alphavbeta3 binding to FGF1 plays a role in FGF1 signaling
- data identify PAI1 as a novel regulator of fibronectin matrix assembly, and indicate that this regulation occurs through a previously undescribed crosstalk between the alphavbeta5 and alpha5beta1 integrins
- Alpha vbeta 6 Integrin promotes the invasion of morphoeic basal cell carcinoma through stromal modulation.
- osteopontin:alpha(v)beta(3)integrin complex untenable as marker of endometrial receptivity and implantation potential
- integrins alphavbeta3 and alpha4beta1 may serve as receptors for sPLA2-IIA and mediate pro-inflammatory action of sPLA2-IIA
- human umbilical vein endothelial cells adhere to immobilized CXCL4 through alphavbeta3 integrin, and also through other integrins, such as alphavbeta5 and alpha5beta1
- Integrin alphavbeta3 is not significantly implicated in the anti-migratory effect of anti-angiogenic urokinase kringle domain
- ability of ovarian carcinoma cells to bind to peritoneal mesothelium in vitro and strongly suggest that vitronectin and its receptors contribute to this crucial event.
- SDF-1alpha enhances the invasiveness of chondrosarcoma cells by increasing alphavbeta3 integrin expression through the CXCR4/ERK/NF-kappaB signal transduction pathway.
- The apo(a) component of Lp(a) signals through integrin alphaVbeta3 to activate endothelial cells.
- urokinase-derived antagonists of alphavbeta5 integrin activation inhibit migration and invasion of carcinoma cells
- These results demonstrate that integrin alphavbeta3-mediated transcriptional regulation of MMP-9 and TIMP-1 is critical for suppressing the ovarian cancer cell invasion.
- VWF strings bind specifically to integrin alpha(v)beta(3) on human endothelial cells
- Analogous to EAE, in subjects with MS, we found increased expression of Opn in DCs and increased expression of the Opn receptors CD44, beta(3), and alpha(v) on T cells.
- analysis of how the different antiangiogenic potential of resveratrol stereoisomers is related to their different capacity to affect alphavbeta3 integrin function
- These data suggest that Coxsackievirus A9 binding to alphaVbeta6 is a high-affinity interaction, which may indicate its importance in clinical infections
- pneumococci exploit the vitronectin-alphavbeta3-integrin complex as a cellular receptor for invasion and this integrin-mediated internalization requires the cooperation between the host signalling molecules ILK, PI3K and Akt.
- data suggest that alphavbeta1 integrin is a functional receptor for hMPV
- Data show that cilengitide activated cell surface alphaVbeta3, stimulated phosphorylation of FAK, Src and VE-cadherin, increased cell permeability and decreased cell adhesion in endothelial cells.