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Validated All-in-One™ qPCR Primer for IRF7(NM_004031.3) Search again
Product ID:
HQP101169
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
IMD39, IRF-7, IRF-7H, IRF7A, IRF7B, IRF7C, IRF7H
Gene Description:
interferon regulatory factor 7
Target Gene Accession:
NM_004031.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq].
Gene References into function
- The structure and function of IRF7 are reviewed
- Stimulation of IRF-7 gene expression by tumor necrosis factor alpha: requirement for NFkappa B transcription factor and gene accessibility
- Preferential binding sites for interferon regulatory factors 3 and 7 involved in interferon-A gene transcription
- acetylation of lysine 92 negatively modulates IRF7 DNA binding
- Interferon regulatory factor-7 synergizes with other transcription factors through multiple interactions with p300/CBP coactivators.
- Interferon regulatory factor 7 regulates expression of Epstein-Barr virus latent membrane protein 1: a regulatory circuit.
- LPS-TLR4 signaling to IRF-3/7 and NF-kappaB involves the toll adapters TRAM and TRIF.
- herpes simplex virus ICP0 blocks interferon regulatory factor IRF7-mediated activation of interferon-stimulated genes; the RING finger domain of ICP0 is essential for this activity
- LPS & HSV upregulate IRF-7 in plasmacytoid dendritic cells. This depends on NF-kappa B activation. Nuclear translocation of IRF-7 occurs rapidly in response to HSV. Activation of IRF-7 contributes to IFN-alpha production in virus-stimulated PDC.
- IRF-5-and IRF-7-induced innate antiviral response results in a broad alteration of the transcriptional profile of cellular genes
- TLR-mediated IFN-alpha induction requires the formation of a complex consisting of MyD88, TRAF6 and IRF7 as well as TRAF6-dependent ubiquitination.
- there are two distinct mechanisms for the activation of the IRF7 promoter, by IFN and by virus infection
- IRF5 and IRF7 are critical mediators of TLR7 signaling
- LPS-induced B7.1 transcription in human monocytic cells may be regulated by JNK-mediated activation of the IRF-7 transcription factor.
- IRF7 and LMP1 interact with each other, and this may relate to the mechanism whereby LMP1 exerts functional effects in B-lymphocytes
- Transduction of active forms of IRF-3 or IRF-7 differentially modulate the apoptotic and antitumor properties of primary macrophages.
- We show that BRCA1, signal transducer and activator of transcription (STAT)-1, and STAT2 are all required for the induction of IRF-7 following stimulation with IFN-gamma.
- expression patterns of IRF3 & IRF7 in normal lymph nodes, reactive hyperplastic lymph nodes & pediatric lymphomas; the number of IRF7-positive cells was found to be elevated in the reactive hyperplastic lymph nodes and pediatric lymphoma
- In addition to its defined role in type I interferon stimulation, IRF-7 plays a key role in modulating adaptive immune responses by inducing low molecular mass polypeptide-2 (LMP-2) expression, either directly or through induction of IRF-1.
- Results report a novel immune evasion mechanism of KSHV vIRF3 to block cellular IRF7-mediated innate immunity in response to viral infection.
- PI3K selectively controls type I IFN production by regulating IRF-7 nuclear translocation in human pDCs
- Our analyses showed a prominent effect of the IRF-7 nonsynonymous SNPs on the risk of developing cirrhosis.
- epigenetic inactivation of the IFN pathway plays a critical role in cellular immortalization, and the reactivation of IFN-regulated genes by transcription factors IRF5 and/or IRF7 is sufficient to induce cellular senescence
- SUMO modification and RIG-I activation are an integral part of IRF3 and IRF7 activity that contributes to postactivation attenuation of IFN production
- TRAF6 and its E3 ligase activity are required for LMP1-stimulated IRF7 ubiquitination.
- This screening demonstrates that LF2 specifically interacts with the central inhibitory association domain of IRF7, and this interaction leads to inhibition of the dimerization of IRF7, which suppresses IFN-alpha production and IFN-mediated immunity.
- efficient IRF7 activation required association with LMP1 CTAR2 in proximity to LMP1 CTAR2 mediated kinase activation sites
- Altogether, these data further highlight the respective functions of IRF-3 and IRF-7 to program apoptotic, immune and anti-tumor responses.