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Validated All-in-One™ qPCR Primer for IL12A(NM_000882.3) Search again
Product ID:
HQP101060
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CLMF, IL-12A, NFSK, NKSF1, P35
Gene Description:
interleukin 12A
Target Gene Accession:
NM_000882.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq].
Gene References into function
- STATE OF ART REVIEW. Malignant B-cells from follicular and marginal zone lymphomas expressed IL-12 p35 and p40 transcripts, whereas only p35 mRNA was detected in mantle cell lymphoma.
- Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p35 secretion is strain and species dependent
- possible role of IL-12 and tissue antioxidants in development and progression of chronic sinusitis
- an alternate mRNA isoform of the human interleukin-12 p35 gene differing from normal p35 transcripts by the deletion of exon 3 is identified
- IL-12 induces expression of the perforin gene in NK cells which is directly regulated by STAT4
- that p35 gene was intact in SLE patients. IL-12 expression in PMN of SLE patients was less prominent than that of the normal controls.
- results demonstrate that interleukin-12(p35) gene activation in the course of dendritic cell maturation involves selective remodeling of a single positioned nucleosome within the promoter containing critical Sp1-binding sites
- A key mechanism in the pathogenesis of MS is the increased expression of CD86 and CD40L and the increased production of IL12 during disease progression.
- Renal immune complex deposition can occur in absence of IL-12p35, but structural renal damage requires presence of IL-12p35 or mediators induced by this molecule, such as IFN-gamma.
- IL-12 can enhance the expression of ICAM-1 in the presence of IFN-gamma and, with IL-18, enhances natural killer anti-tumor activity
- IL-12(p35) transcriptional repression in neonatal dendritic cells takes place at the chromatin level.
- IL12 does not activate STAT4 signaling in human vascular endothelial cells
- Decreased LPS-induced IL-12(p70) and IL-10 responses were associated with the TLR4 (Asp299Gly) polymorphism and independently with asthma, especially atopic asthma in school-age children
- interleukin 12 and interferon gamma are involved in inhibition of cytotrophoblastic cell invasion
- interleukin-12 p35 gene transcription is activated by interferon regulatory factor-1 and interferon consensus sequence-binding protein
- TSLP is a major regulatory cytokine for CD40 ligand-induced IL-12 production by DCs, and TSLP-activated DCs could promote the persistence of Th2 inflammation even in the presence of IL-12-inducing signals
- iC3b interferes with monocyte-derived dendritic cell differentiation and IL-12 and IL-10 production is mediated via an ERK MAPK-dependent mechanism
- IL12A gene polymorphisms may affect the final steps of gastric carcinogenesis in H pylori infected subjects.
- novel anti-inflammatory property of recombinant HBsAg which involves inhibition of interleukin 12.
- This review summarizes information on the expression and role of IL-12 both in patients with Crohn's diease and experimental models of colitis, thus emphasizing differences between IL-12 and IL-23 activity on the development of intestinal inflammation.
- Our results suggest that IL-12 and IL-18 contribute to the establishment of Th1 polarization seen in FMF and play a part in its pathogenesis.
- Interferon-gamma and bacterial lipopolysaccharide act synergistically on human neutrophils enhancing interleukin-8, interleukin-1beta, tumor necrosis factor-alpha, and interleukin-12 p70 secretion and phagocytosis via upregulation of toll-like receptor 4
- The positive expressions of IL-12 and IL-18 can play an important role in progression and metastasis of gastric cancer, and IL-12 might be an independent factor of poor prognosis in gastric carcinoma.
- mRNA levels of IL12 subunit p35 in active SLE patients was significantly higher compared with those in the inactive SLE patients
- Our data suggest that the intestinal microvasculature is responsive to ligands of TLR3 expressed on intestinal endothelial cells, thereby adding to the regulation of adaptive immunity and leukocyte recruitment.
- In primary Sjogren's syndrome, infiltration of the salivary gland by macrophages and dendritic cells and expression of IL-18 and IL-12 play active roles in infiltrative injuries and have a correlation with predictors of lymphoma development.
- Single nucleotide polymorphisms and haplotype of interleukin-12A are not associated with hepatitis B virus persistence and development of hepatocellular carcinoma.
- probiotic S. thermophilus and Leuconostoc strains are more potent inducers of Th1 type cytokines IL-12 and IFN-gamma than the probiotic Lactobacillus strains
- TAP1 and TAP2 expression restores both antigen presentation and immunogenicity in A375 melanoma cells and concomitantly increases IL-12 and IFN-gamma production in tumor antigen-specific cytotoxic T lymphocytes.
- These observations suggest that lower measles-specific T-cell immune responses elicited by measles vaccine in infants may be due to diminished levels of IL-12 and IL-15.
- anti-CD3/CD28 enhances vaccine efficacy by limiting the regulatory T cell response and promoting expansion of activated effector cells
- The gene expression of IL-12 p35 was significantly higher in periodontitis compared with gingivitis.
- study demonstrates that oxidative stress induced by soluble cigarette smoke components potently inhibits the production of IL-12 and IL-23 by maturing dendritic cells
- IL-12 promotes disease development in acute inflammatory demyelinating polyradiculoneuropathy.
- Results suggest that IL-12, a principal TH1 cytokine with potent anti-angiogenic activity, is the mediator of angiostatin's activity.
- The production of IL-12, IL-18, and TNF-alpha by alveolar macrophages was increased in patients with both acute and chronic forms of Hypersensitivity pneumonitis in either the absence or presence of lipopolysaccharide compared with controls