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Validated All-in-One™ qPCR Primer for IL4(NM_000589.3) Search again
Product ID:
HQP101030
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BCGF-1, BCGF1, BSF-1, BSF1, IL-4
Gene Description:
interleukin 4
Target Gene Accession:
NM_000589.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq].
Gene References into function
- Increased IL-4 expression in Mycobacterium tuberculosis-activated lymphocytes promotes CD30 expression, which sensitizes the lymphocytes to TNF-alpha-mediated apoptosis via TRAF2 depletion.
- expression in T cells up-regulated by CD30L
- Incubation of cultured bronchial epithelium with IL-4 causes marked inhibition of the amiloride-sensitive Na+ channel, as well as up-regulation of the cyclic AMP-dependent current demonstrated by an increase in chloride secretion and CFTR mRNA level.
- high-affinity interaction of human IL-4 and the receptor alpha chain is constituted by two independent binding clusters
- Characterization of IL-4 receptor components expressed on monocytes and monocyte-derived macrophages: variation associated with differential signaling by IL-4
- role in regulation of Toll-like receptor surface expression in human peripheral blood monocytes and B cells
- The acetylation state of histones associated with the IL-4 promoter is a key regulator of IL4 gene expression in T cells.
- Oligonucleotide fishing for STAT6: cross-talk between IL-4 and chemokines.
- Interleukin (IL)-4 inhibits phorbol-ester induced HIV-1 expression in chronically infected U1 cells independently from the autocrine effect of endogenous tumour
- The germinal center (GC) B cells in tonsils and colonic mucosa are a predominant source of IL-4 and demonstrate the reliance of IL-4-dependent Th2 differentiation in vivo on strong B cell participation and the development of GCs.
- regulates CCR5 amd CD4 expression and HIV-1 replication in human macrophages and microglia
- IL-4 interfered with the TGF-beta-induced transcriptional activation of the CTGF gene.
- Polymorphisms for interleukin-4 (IL-4) -590 promoter, IL-4 intron3, and tumor necrosis factor alpha -308 promoter: non-association with endometriosis
- CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells
- Acute cardiac transplant rejection is associated with low frequencies of interleukin-4 producing helper T-lymphocytes rather than with interleukin-4 promoter or splice variants.
- Cysteinyl leukotrienes acting on human eosinophils can act as autocrine or paracrine mediators to stimulate the rapid, nonexocytotic release of preformed IL-4.
- increased HIV replication in the thymus by inducing differentiation and expansion of mature CD27(+) thymocytes expressing CXCR4 or CCR5
- results indicate opposite effects of IFN-gamma and IL-4 on VEGF expression from normal and activated HF and HK
- IL-4 was found to down-regulate IFN-gamma and tumor necrosis factor alpha, and HIV-1 p17 restored the ability of cells to produce both cytokines
- data are consistent with a reduced capacity to make IL-4 promoted antibody responses to exogenous antigen in early pre-diabetes
- Association of interleukin-4 and interleukin-1B gene variants with Larsen score progression in rheumatoid arthritis.
- There is correlation between the Chinese ulcerative colitis patients and the gene polymorphisms of intron 3 of IL-4.
- B-CLL patients with progressive disease had a significantly increased number of T cells spontaneously producing IL-2, IL-4 and GM-CSF, suggesting a role for T cells in the pathogenesis of B-CLL.
- These results show that p38 MAPK provides a costimulatory signal for IL-4-induced gene responses by directly stimulating the transcriptional activation of STAT6.
- During the latent phase of HSV-1 infection, IL-4 expression was significantly elevated in BALB/C mouse brains when compared with the uninfected controls.
- DNA methylation around the IL-4 and IL-13 genes before and after T cell differentiation
- Vitamin E inhibits IL-4 gene expression in peripheral blood T cells by blocking binding of transcription factors to two important IL-4 promoter binding sites for NF-kappaB and AP-1 and interfering with promoter activity upon T cell activation.
- results suggest that in DG75 cells, TRAF3-induced MEK1 activation may be involved in CD40-mediated upregulation of IL-4-driven germline C epsilon transcription
- expression of mRNA for IL-4, IL-5, TGF-beta1, IFN-gamma in patients with cicatricial pemphigoid
- IL-4 induction requires c-jun N-terminal kinase
- a common haplotype of the gene is associated with severe respiratory syncytial virus disease in Korean children
- mediates STAT6 signaling; regulated by protein phosphatase 2a
- IL-4 intron 3 polymorphism is not a useful marker for prediction of the susceptibility of febrile seizure and epilepsy of children.
- lack of role in modulating thymus and activation-regulated chemokine/CCL17 and interferon-induced protein of 10kDA/CXCL10 release by tumor necrosis factor-alpha and interferon-gamma in HaCaT cell line
- IL4 regulates differentiation and maturation of dendritic cells in cooperation with DC21 and IL-12
- IRF-1 and IRF-2 induced by IFN-gamma bind to three distinct IL-4 promoter sites and function as transcriptional repressors
- Human IL-21 and IL-4 bind to partially overlapping epitopes of common gamma-chain.
- Polymorphisms in the IL-4 gene did not reveal any association with increased risk of developing rheumatoid arthritis in Taiwan Chinese.
- induces human germinal center-associated lymphoma gene
- Lack of antitumor activity and intolerance of this drug in patients with advanced HIV disease and Kaposi's sarcoma.
- A non-secreted Il-4 variant whose expression is tightly associated with deprivation apoptosis was detectd in T-cells. This variant is the major form detected in HIV-infected patients.
- IL-4-stimulated A549 lung adenocarcinoma cells are committed to apoptosis via the caspase-3 pathway involving the binding of 15(S)-HETE to PPAR gamma transcription factor, the down-regulation of Bcl-XL, and the resultant overexpression of Bax.
- Protease allergen Der p1 and the secreted proteases of the hookworm Necator americanus are able to directly induce type 2 cytokines IL-4, IL-5, and IL-13 by basophils, that could promote IgE synthesis, eosinophil recruitment, and Th2 cells.
- IL-4 gene polymorphisms are associated with disease susceptibility and disease progression in IgA nephropathy in Japanese patients.
- IL-4 alone is sufficient to drive activation-induced expression of cytidine deaminase via JAK/STAT pathways in primary B cells.
- association between RPI/RPI and T/T homozygous carriage and patients with discoid rash in systemic lupus erythematosus
- All nine patients expressed all of the TLRs studied, whereas only five out of the nine patients had any granulomas positive for IL-4.The associations between TLRs 1, 5, and 9 were different in IL-4-negative compared with IL-4-positive patients.
- In conjunctiva of keratoconjunctivitis sicca patients.
- Inhibition of a rapid anaphylatoxin-induced mobilization of monocytes and dendritic cells to inflamed tissues represents an important anti-inflammatory activity of the Th2 cytokine IL-4.
- Genetic variants of IL4 could contribute to the development of chronic disseminated candidiasis in adult patients with acute leukemia
- the anti-inflammatory cytokine IL-4 modulates the life span of monocytes by an extracellular signal-regulated kinase-dependent pathway
- IL-4 regulates osteoclast development by regulating gene expression, including RANK
- Constitutive expression of BPI, which increases upon interleukin 4 stimulation, by human dermal fibroblast was demonstrated, suggesting an important role of BPI in gram-negative bacterial clearance and a dampened response to endotoxin in the skin
- IL4 is associated with diabetes mellitus, type 1 in Filipinos.
- In all three groups of patients significant correlation was seen between abundance of IL-2 vs. IL-4, IL-5, IL-10, or TNF-alpha, between IL-4 vs. IL-10, and between TNF-alpha vs. INF-gamma
- The IL-4 gene locus (-590 C-->T polymorphism and 70 bp repeat polymorphism) contributes little to the interindividual susceptibility for adult periodontitis in a Korean population.
- expression of alleles of the genes encoding IL-2, IL-3, IL-4, and IL-13 in CD4(+) T cell clones
- No genotypic effects on total serum IgE levels.
- Blocking of IL-4-induced activation of epithelial Stat6 by Helicobacter pylori in vitro may represent an evolutionarily conserved strategy to disrupt a Th2 response and evade the host immune system, allowing for successful chronic infection.
- IL-4 gene polymorphism are associated with allergic asthma in a Chinese population.
- Single Nucleotide Polymorphism in interleukin 4 gene is associated with AIDS progression
- Number of CD8+/IL-4 cells significantly higher in young than in elderly untrained.
- results show that IL-4 inhibits endothelial cell growth by altering the expression of cell cycle regulatory molecules p53, p21(Waf1), cyclin D1 and cyclin E
- Treatment of airway epithelial cells with IL-4 in combination with TNF-alpha increases the stability of eotaxin mRNA.
- interleukin-4 has emerged as promising means of improving allergic states, and several antagonists have been developed, among which soluble IL-4 receptor is now in human trials--REVIEW
- IL-4 binds with high affinity to its receptor on transiently transfected hepatocellular carcinoma (Hep3B) cells, leading to a suppression of hepatitis B virus gene expression and replication.
- Autocrine production of IL-4 and IL-10 promotes thyroid tumor cell progression and resistance to chemotherapy through the up-regulation of antiapoptotic proteins.
- Possible involvement of single nucleotide polymorphisms in IL4 gene in development of asthma and regulation of total serum IgE.
- skin mast cells precultured in the presence of IL-4 [alone or plus stem cell factor (SCF)] before anti-IgE stimulation, acquired the ability to produce IL-5, and IL-1beta was concomitantly suppressed
- IL-4 up-regulates SOCS-1 expression in A549 human lung adenocarcinoma cells by stimulating interaction of STAT6 with three interferon-gamma-activated sequence (GAS) motifs in the SOCS-1 promoter.
- These results indicate that TNF-alpha and IL-4 synergistically up-regulate the expression of IL-13Ralpha2 decoy receptor on human fibroblasts
- there is increased production of IL4 by PHA-activated PB lymphocytes in Hodgkin lymphoma. The CD3(+)CD8(+) T-cell population appears to be responsible for this increased synthesis.
- Interleukin 4 gene polymorphisms is associated with incident stroke
- MC(T) type of mast cell (MC) from lung is sensitive to IL-4-induced apoptosis, whereas MC(TC) cells from both skin and lung are resistant; most in vitro derived mast cells from cord blood and fetal liver progenitors also are IL-4-sensitive.
- Results describe the effect of CD80 and CD86 inactivation on interleukin-4 and interferon-gamma production in nonatopic asthmatics and healthy subjects.
- IL-4 signaling is regulated by TGF-beta through targeting NF-kappaB signaling
- Significantly higher levels of il-18 were noted during the acute phase of infection among children with an increased incidence of respiratory infections.
- In maternal peripheral blood, the expression of IL-4 mRNA was up-regulated from the second gestational month (GM) to delivery
- IL-4 could account for proinflammatory AM phenotype changes and B7 surface-marker shifts, as seen in subjects with systemic sclerosis
- Results indicate that interleukin-4, together with recombinant human stem cell factor, can induce T cell maturation from cord blood progenitor cells, and that IL-4 increased the expression of FcepsilonRI on fetal liver mast cells.
- When parasite antigen-specific IgE is present in a filaria-infected individual, basophils function to amplify the ongoing Th2 immune cell response by releasing IL-4 in greater amounts and more frequently than CD4+ T cells in response to filarial antigen.
- IL-4 -589-C/T polymorphism is not an important determinant of HIV-1 disease progression in children.
- Th1/Th2 differentiation and in a transgenic mouse line containing the human IL-4/IL-13 genes for intergenic transcripts
- polymorphisms were not related to periodontal disease susceptibility in African-American Brazilian population
- IL-4 induces the specific goblet cell product trefoil factor-3 (TTF3) expression via a direct effect on intestinal epithelial cells, through a STAT6- and de novo protein synthesis-dependent pathway.
- variants in the IL4, IL13, and IL4RA genes play an important role in controlling specific IgE response in atopy
- IL-4 induced expression of fractalkine in fibroblasts, and subsequent monocyte chemotactic activity in the culture supernatant
- IL-4 mRNA expression was identified in only two of the eight very mild lesions, one of the ten subacute lesions, and none of the ten lichenified lesions in atopic dermatitis
- Alexithymia and difficulty in identifying feelings could be associated with increased levels of interleukin-4
- Maternal and fetal carriage of the interleukin-4 T allele is associated with an increased risk of spontaneous preterm birth in multifetal gestations.
- Activation of T cells and IL-4 production may be involved not only in the basic pathogenesis of childhood asthma but also in its acute exacerbation.
- Patients homozygous for GG at single nucleotide polymorphism -1082IL10 (high IL-10 producers) and HLA class I mismatched (but matched for class II) are protected from chronic rejection.
- IL-4 strongly inhibits chemotherapy- and CD95-induced apoptosis through up-regulation of anti-apoptotic proteins Bcl-xL and cellular FLIP, suggesting that IL-4 production may contribute to apoptosis resistance of human solid tumors during Th2 responses.
- the observed polymorphisms, resulting in low IL-10 and high IL-4 gene transcription, could favor a T helper (Th)-2 mediated antibody response to neoantigenic stimulation associated with hepatotoxicity susceptibility.
- IRS-2-dependent IL-4 signaling in macrophages is impaired in models of type 2 diabetes mellitus through a mechanism that relies on insulin/glucose-dependent Ser/Thr-Pro motif serine phosphorylation mediated by the mTOR pathway
- In a transgenic mouse model, an IL-4 signal is required at the early stage of T-cell receptor-mediated T cell activation for lineage commitment to Th2 cells, along with structural changes in chromatin.
- Th2 cytokines IL-4 and IL-13 downregulate paxillin production by BAEC, thereby disrupting the cell-matrix interactions
- There were no differences in IL-4 -590C/T genotypes and allele frequencies between control women and all patients with endometriosis or between control women and each subgroup of patients with endometriosis.
- demonstrated induction of IgE synthesis by the interaction between adenosine-stimulated mast cells and B lymphocytes involves up-regulation of IL-4 and IL-13 mediated by A(2B) receptors
- Interferon gamma showed a striking increase in the proportion of T cells that secreted IL-4 upon challenge.
- IL-4 alone, in the absence of detectable endogenous GM-CSF, transforms human peripheral blood monocytes to a CD1a(dim) dendritic cell (DC) subset that could be matured to CD83(+) DCs
- IL-4 VNTR allele is not a major genetic regulator in recurrent pregnancy loss
- FN-gamma regulates IL-4- and STAT6-dependent signaling and gene expression in airway epithelial cells by multiple mechanisms
- at a lower maturation stage, IL-4 acts as a negative regulator of the mast cell lineage, but that this property disappears or is even reversed upon terminal differentiation of the cell.
- exposure of human mast cells to IL-4, IL-5, and IFN-gamma during growth and differentiation generally down-regulated mast cell number and function, whereas IL-4 increased mature mast cell division and degranulation.
- IL-4 and IL-13 secreted from embryo during implantation may up-regulate TARC by endometrial epithelial cells.
- IL-4-producing cells are decreased in Behcet's disease
- In monocytes IL-4 has a dual function: to inhibit pro-inflammatory cytokine production and to suppress spontaneous apoptosis.
- IL-4 and IL-13 mediate the hypercontractility of intestinal muscle via a STAT6 pathway at the level of the smooth muscle cell. The STAT6 pathway may contribute to the hypercontractility of intestinal muscle in Crohn's disease.
- IL-4 gene polymorphism is dependent on HLA-DRB1 genotype in patients with giant cell arteritis.
- distinct activity of the IL-4 signaling pathway in diffuse large B-cell lymphoma subtypes
- No significant association of the genotype or allelic frequency of IL-4 polymorphism with duration of intermittent mandatory ventilation.
- Although IL-4 has been reported to influence natural killer (NK) cell differentiation, mice deficient in IL-4 have normal NK cell numbers, phenotype, and effector functions.
- IL-4-producing cells inhibit the development of acute graft vs host disesase.
- IL-4 > PI3K/Akt > NF-(kappa)B signaling pathways, which activate androgen receptor signaling, play an important role during the development of androgen independent prostate cancer cells.
- p100 has an important role in the assembly of STAT6 transcriptosome, and that p100 stimulates IL-4-dependent transcription by mediating interaction between STAT6 and CBP and recruiting chromatin modifying activities to STAT6-responsive promoters
- IL-4 may act as an autocrine growth factor in pancreatic cancer cells.
- NK cells exposed to IL-4 do not exert DC selection, may impair efficient Th1 priming, and favor either tolerogenic or Th2-type responses
- transepidermal water loss was suppressed by TNF-alpha and IFN-gamma, and these effects were also inhibited by IL-4
- Reduced vaginal levels of anticandidal factors in IL-4*T homozygotes may increase susceptibility to recurrent vulvovaginal candidiasis.
- TH2-cytokines interleukin-4 and -13 have roles in regulating the gene expression pattern of human peripheral blood monocytes
- Retinoic acid inhibits CD40 plus IL-4 mediated IgE production through alterations of sCD23, sCD54 and IL-6 production
- Interleukin-4 level has no correlation with the course of subacute sclerosing panencephalitis or its treatment, when the age, disease stage, and clinical condition of the patient are considered.
- The IL-4 gene -590 C/T polymorphism is associated with oral cancer.
- IL-21 reduced IL-4-driven IgE synthesis by mitogen-stimulated human PBMC
- African-Americans with the IL4 GCC haplotype had greater risk of spontaneous preterm birth. White mothers with the IL4 CC haplotype had reduced risk of SGA. Carriers of the IL4(-589)T variant had increased risk of SGA for both groups.
- These data suggest that IL-4, IL-13 and IFN-gamma play an important roles in penicillins allergy.
- These data suggest that during a respiratory virus infection activated CD8+ T cells from asthmatic subjects may produce excess type 2 cytokines and may contribute to asthma exacerbation by augmenting allergic inflammation.
- IL-4 acts directly on mature osteoclasts and inhibits bone resorption by inhibiting NF-kappaB and calcium signaling.
- IL-4 regulates TNFalpha-induced IL-8 expression at a transcriptional level and this mechanism involves STAT6 and NF-kappaB transcription factors
- Human IL-4 protects porcine endothelial cells from the cytotoxic effects of human complement via activation of the phosphatidylinositide 3-kinase/Akt signaling pathway
- IL-4 is expressed on CD8(+) T cells and may have a role in chronic graft-versus-host disease
- a significant association of the IL-2 -330 TT genotype and of the IL-4 -590 CC genotype with schizophrenia could be identified
- intracellular vesicular system mediates the rapid mobilization and secretion of preformed IL-4 by activated eosinophils
- In females the TLR4 and IL4 genes show an epistatic effect on the risk of asthma.
- pro-inflammatory and pro-atherogenic effect of IL-4 in vascular tissues
- Certain IL-13/l4 haplotypes associated with severe Respiratory Syncytial virus infection.
- HIV p17 was able to counteract the anti-inflammatory effects of IL-4 on the TNF-alpha and IFN-gamma production in IL-15 stimulated monocytes
- The interaction between SNPs in IL-4/IL-13 pathway significantly influence genetic control of serum IgE levels and development of childhood asthma.
- IL-4 is identified as a pro-migratory agent for myogenic cells by a mechanism which partly explains the demonstrated activity of IL-4 during differentiation.
- a cytokine receptor, the IL-4 receptor alpha chain, mediates eotaxin-stimulated mobilization of preformed IL-4 from eosinophil granules into secretory vesicles
- Data show strong expression of interleukins (IL)-4, IL-10, and IL-13 in different non-leukocytic cell types, especially in endothelial and epithelial cells in the rat, with strong similarity of expresion in humans.
- This is the first demonstration of sex-specific association of the two foremost genes of the IL-4 signalling cascade with chronic inflammatory arthropathies.
- IL-4 has the capacity to inhibit COX-2 mRNA transcription in NSCLC cells and the inhibition of PGE(2) appears to be predominately COX-2 dependent.
- ROC analysis demonstrated that the ratio of IL-5/IL-4 was the most sensitive parameter associated with acute graft-versus-host disease.
- IL-4 has differential effects in coronary artery smooth muscle cells: short-term exposure enhances OPG production through a STAT6-dependent mechanism, but long-term exposure causes Cbfa1-dependent osteogenic transformation and decreased production of OPG
- A structure/function analysis of the IL-4 ligand-receptor interaction, using various mutations of both the ligand and the receptor, is reported.
- sIL-2R and IL-6, but not IL-2, IL-4, or IL-10, may have a role in preventing progression of aggressive non-Hodgkin's lymphoma
- A(2B) receptors up-regulate IL-4 through G(q) signaling that is potentiated via cross-talk with G(s)-coupled pathways.
- Mast cells from the human mast cell line HMC-1 express IL-4 delta 2 isoform, an alternative splice variant of human IL-4.
- Induces production of CCL17/thymus- and activation-regulated chemokine (TARC) in human T-cells.
- M. bovis BCG-infected human epithelial cells can have an active role in a local inflammatory immune response via the secretion of IL-4, CXCL-8 and CXCL-10, which can be selectively regulated by Th2-derived cytokines
- Activation of STAT6 appears to be a key factor in P-selectin expression induced by substance P and IL-4 because treatment with STAT6 decoy oligodeoxynucleotides significantly inhibited P-selectin expression.
- IL-4 up-regulates mitochondrial adenine nucleotide translocase (ANT)3 in human T cells.
- The possible role of the polymorphisms in IL-4 gene promoters (C-33T, C-590T and G-1098T) in modulating allergic response and asthma in the Russian population, was studied.
- A significant increase in circulating Vdelta1 T lymphocytes producing interleukin-4 (IL-4) was found in patients with FL, MT, and MZ NHL, at variance with DLCL and HR B-CLL
- Both IL-4 and IL-13 inhibited the expression of CXCR4, in contrast to CCR5, which was inhibited by IL-13 alone.
- The IL4 C-590T polymorphism was not associated with risk of any stage of premaligant lesions, except for a slight, statistically insignificant inverse association of the C allele with dysplasia.
- expression of CXCR5 identifies a unique subset of Vgamma9Vdelta2 T cells which express the costimulatory molecules ICOS and CD40L, secrete IL-2, IL-4, and IL-10 and help B cells for Ab production
- study of link between SWA-specific Th2 cytokines IL4 and IL5 responsiveness and subsequent increases in worm-IgE, and age factors
- IL4 polymorphism is associated with protection against nasal polyps in a Korean population.
- IL-4 induced up-regulation of HIF-1alpha gene transcription and the PI3K signaling pathway
- There is an association between expression of Th1/Th2 transcription factors and cytokines (T-bet, GATA-3, IFN-gamma, IL-4,IL-18) in systemic lupus erythematosus.
- It's suggested that the IL-4 promoter polymorphism -590 C/C is associated with the expression of 5-LO in the patients with nasal polyp.
- Our findings of higher frequency of IL4 and IL4RA genotypes and alleles with rheumatoid arthritis.
- findings demonstrate for the first time an association between IL-4 590 promoter polymorphism and contracting brucellosis in the Iranian population
- higher risk in ESRD patients with low IL-4 and high IL-6 producing genotypes
- Polymorphism in the promoter of the IL-4 gene is associated with asthma and is a disease modifier in terms of the severity of AHR.
- TLR-activated DCs inhibit early IL-4 production by CD4 T cells and thus inhibit their ability to subsequently increase GATA-3 expression and commit to the Th2 lineage
- Tuberculosis BCG vaccine did not affect mRNA expression.
- an influence of genetic variability at the promoter of IL-4 gene (-590C>T) and a coding region of IL-13 gene (R130Q) on the occurrence of allergic asthma and no relationship between IL-16 promoter polymorphism (-295T>C) and this disease.
- the gene polymorphisms of the promotor region of IL-4 at position (-1098) and (-33) are likely to play a pathogenic role in idiopathic pulmonary fibrosis and in modification of its clinical presentation and severity
- IL4-induced FBGC are characterized by the expression of alphaMbeta2, alphaXbeta2, alpha5beta1, alphaVbeta1, alpha2beta1, and alpha3beta1, which indicates potential interactions with fragments of C3, fibrin(ogen), fibronectin, Factor X, and vitronectin
- Data showed that the frequencies of IL-4 (T-1098) alleles are more than 0.95.
- Collectively, these data suggest a link between the inducible phenotype of CCL23 expression in monocytes by the prototype Th2 molecule pair IL-4/STAT6 and the increased number of CCL23-expressing cells in skin of atopic dermatitis patients.
- IL4-590 C/T polymorphism may play a role in the balance between IL4 and IFN-gamma, as well as in the control of parasitemia, which in turn may alter the severity of malaria
- the duplicitous effects of IL-4 on tumour immunity depend on the type of effector cell (adaptive/innate) mediating tumour clearance and whether tumour growth depends on stromal infrastructure [review]
- study highlights protective role of IL-4 during S. aureus corneal infection
- The Single Nucleotide Polymorphisms tested in this study within the region coding for IL-4 fail to show significant associations with susceptibility to IIM disease.
- IL4+33 and IL4R*Q551 polymorphisms may have a promoting role of TH2 mediators in African MS descendants. IL4 and IL4R genes are susceptibility factors for Brazilian MS but may be able to modify ethnicity-dependent disease risk and penetrance.
- the IL4 T/T genotype was associated with an increased risk for the development of oral squamous cell carcinoma (P = .018; OR 1.47, 95% CI 0.66-3.28), especially for early stages of this malignancy
- IL-4 induces dose-dependent early activation of extracellular signal-regulated kinase (Erk)/Akt kinase as well as STAT6 in Jurkat T cells.
- study demonstrated an association between the IL-4 -590 T/T and IL-4 -34 T/T genotypes and aggressive periodontitis
- IL-4 expression is elevated in severe recurrent hepatitis C and may play a role in the progression of hepatic lesions after liver transplantation.
- Some cytokine gene alleles reflecte stable graft function among kidney transplant recipients.
- Neither IL-4 genotype nor the serum levels seem to play any role in recovery from coronary artery bypass surgery.
- The inducible mRNA expression and the promoter activity of IL-4 were significantly diminished by ectopic expression of T-bet.
- IL4 and IL4R polymorphisms and haplotypes were neither significantly associated with IgE levels in controls nor associated with glioma status
- Levels of serum IL-4 was considerably higher in asthmatics than non-asthmatic controls
- Data suggest that individuals who have IFN-gammaAA genotype are more susceptible, and those who carry IL-4CC genotype are more resistant to brucellosis.
- There was no evidence of a statistically significant association in the allelic distribution of IL4, IL10, IL16 and TNF polymorphism that were tested,nor in the haplotype frequencies in patietns with CD.
- ST2 reduced production of IL-4, IL-5, and IL-13 from IL-33-stimulated splenocytes. These results indicate that soluble ST2 acts as a negative regulator of Th2 cytokine production by the IL-33 signaling.
- Polymorphisms of the IL-4 (promoter-590 and intron 3) and TNF-alpha promoter-308 genes are not related to gout in Chinese patients in Taiwan.
- IL-4 and interferon gamma have opposite effects on the expression of claudin-2 and the physiology of the tight junction
- S100A8 is regulated by IL-17, dexamethasone, IL-4 and IL-13 in HaCat cells (human keratinocyte cell line)
- the interferon-gamma +874(A/T) and IL-4 VNTR in intron3 polymorphisms are not responsible for genetic susceptibility to ITP
- Results report that interleukin-4 (IL-4) induced the expression of 15-LO-1 in human cord blood derived mast cells (CBMC).
- -590*C allele significantly increased in Korean psoriasis patients, especially late-onset group
- polymorphisms were not related to severe generalized chronic periodontitis in a Turkish population
- Interleukin 4 promoter polymorphism may be contribute to the genetic predisposition and to the pathogenesis in MS.
- Interleukin-4 may modulate the expression of chloride channel protein CLC-2 and CLC-3 in chronic rhinosinusitis without nasal polyps.
- The results indicated that IL-4-treated LS174T cells are able to produce mucins with a higher degree of O-glycosylation than untreated counterparts.
- The ratio of IL-4delta2 to IL-4 mRNA expression may play a role in disease severity for pulmonary tuberculosis patients. From our observations IL-4 mRNA expression was attenuated in patients with pulmonary infection, either tuberculosis or pneumonia.
- IL-4 suppressed the activation of caspases 9 and 3, and, in contrast to previous reports, induced the phosphorylation (deactivation) of BAD.
- IL-4 plays an important role in promoting androgen-independent prostate cancer growth
- we studied the association of variants in IL-4 (C-589T, G3017T) and IL-4R alpha (Gln576Arg) with allergies and with risk of pancreatic cancer
- production of human interleukin-10 (IL-10) and IL-4 partially contributed to HSV-1-induced KSHV replication.
- IL-4 gene polymorphisms that arise at either intron 3 or promoter -590 positions are not suitable genetic markers for autoimmune diseases of the thyroid gland among Taiwanese Chinese.
- Location of IL4 in the 5q31-33 complex genetic region requires cautious interpretation of previous IL4-association studies, since 5q31 contains many genes involved in immunological responses, and prior linkage disequilibrium data may be misleading.
- role of TNF-alpha-308 G/A and IL-4-589 C/T polymorphisms in susceptibility to and severity of asthma.
- gastric epithelial cells might regulate the balance between tolerance and immune response by the fine tuning of IL-4 and IL-4delta2 expression
- IL-4 and IL-4RA gene polymorphisms concur in selecting the H. pylori infecting strain, probably influencing the IL-4 signalling pathway
- IL-4 acts as an autocrine survival factor in epithelial cells. Results indicate that inhibition of IL-4/IL-4R signaling may serve as a novel treatment for epithelial cancers.
- IL-4 and IL-13 potently induce fibroblast growth factor 2-primed bronchial smooth muscle cell (BSMC)proliferation; could have a potential role in BSMC hyperplasia.
- Downregulation of E6 and E7 levels by IL-4 in CaSki cells is weaker than that exerted by TGF-beta1, a known LCR inhibitor.
- IL-4 gene expression-related polymorphism is associated with the development of initial stages of colorectal cancer, while in advanced stages IL-4 levels appear to be less important
- Specific cytokines (IL-1alpha, IL-4, and IL-6) are acutely elevated during hyperglycemia in children with type 1 diabetes, and these elevations persist for hours after hyperglycemia has been corrected.
- The results suggest that the interleukin-4 (C-590T) and interferon-gamma (G5644A) gene polymorphisms may not be associated with the susceptibility of Iranian individuals to periodontitis.
- The risk of progression and chronic course of viral hepatitides in Caucasian population was associated with T/T genotype of the polymorphic region C590T in the IL-4 gene.
- Molecular and structural basis of cytokine receptor pleiotropy in the interleukin-4/13 system are reported.
- IL-4 and IL-13 induced osteoprotegerin(OPG) expression through activation of STAT6 in endothelial cells, and HUVEC-derived OPG is an IL-4/IL-13-induced inhibitor of osteoclastic resorption
- IL-4 polymorphism was not a primary predictor of oral squamous cell carcinoma development
- Data show that depressed and anxious mood were both associated with significantly lower ratios of polyclonally-stimulated CD4 and CD8 (+) cells producing IFN gamma (TH(1) cells) vs. IL-4 (TH(2) cells)in ovarian cancer patients.
- TNF-alpha-positive cells are more frequent, while IL-4-positive cells are less frequent in tissues from lung neoplasms
- These findings, together with the observation of constitutive Stat6 activation in many human malignancies, suggest that Stat6 activities could be a biomarker for cancer cell's invasive/metastatic capability.
- Results suggest that IL-4 -590 C/T and 8375 A/G SNPs do not confer a relevant role in the susceptibility of Kawasaki disease or coronary artery lesions development in Taiwanese children.
- The -590CC genotype in the IL4 gene is a strong predictive factor for the development of hypothyroidism in Hashimoto disease.
- A model is proposed that involves cooperative binding of IL-4 to monocyte cell surface dermatan sulfate (CSB), facilitating an important role of glycosaminoglycans in the IL-4-driven differentiation of monocytes into dendritic cells.
- Data show that IL-4-induced Stat6 activities affect apoptosis and gene expression in breast cancer cells.
- The stepwise analyses showed that IL-4 was the biomarker in exhaled breath condensate which contributed most to the discrimination between controls and systemic sclerosis patients.
- These data suggest that colon tumor growth is dictated by stem-like cells that are treatment resistant due to the autocrine production of IL-4.
- S100/A11 is significantly downregulated in the presence of IL-4 and IL-13.
- Association with clinical course of the disease (typical form) was obtained for 150V polymorphism of IL4RA gene and for F/f polymorphism of VDR gene (p = 0.001 and p = 0.009, respectively).
- This meta-analysis suggests there may be an important effect of single nucleotide polymorphisms (SNPs) in the promoter region of IL-4 gene on the pathogenesis of atopic asthma.
- Human bronchial smooth muscle cells contain functional BK(Ca) channels whose activity is rapidly potentiated by the cytokine, IL-4, but not by IL-13.
- African-American infants exposed to environmental tobacco smoke having interleukin 4 C-589T polymorphism were at increased risk of wheezing.
- patients with multivessel coronary artery disease had a higher periprocedure(percutaneous coronary angioplasty) ratio of IL-4(interleukin 4) than those with single-vessel coronary artery disease
- IL-4 intron 3 VNTR and IL-6 (-174) G/C variants reflect ethnic variation.
- activation of the TRPM8 variant in human lung epithelial cells leads to increased expression of IL-1alpha, -1beta, -4, -6, -8, and -13, granulocyte-macrophage colony-stimulating factor (GM-CSF), and TNF-alpha.
- the SNPs IL4-590 and IL6-174 may be useful markers for colorectal cancer prognosis
- IL-4 gene polymorphism is associated with the risk of BPH
- IL-10, IL-4 and TNF-alpha genetic polymorphisms were not related to the risk of developing drug induced liver injury
- IL-13 attenuates EC viability by inducing apoptosis, and STAT6 plays pivotal roles on IL-13- and IL-4-induced apoptosis in ECs.
- There was no significant difference in IL-4/beta-actin between the patients with AIH and the healthy controls. In contrast there was a significant difference of IFN-gamma/beta-actin between those two groups.
- There was no difference in relation to IL-2 and IL-4 promoter polymorphism influencing renal allograft survival.
- studied the expression of IL-1beta, IL-4, and IL-8 in the gingival crevicular fluid (GCF) of children, adolescents, and young adults with and without fixed orthodontic appliances
- Data show that there is no significant association of IL-4 gene with schizophrenia in Japanese population using either single marker or haplotype analyses.
- mechanisms by which IL-4 mediated cell cycle progression in T-ALL cells
- Serum IL-4 appears to be an early marker of immunological response to sublingual immunotherapy.
- proliferation of endometriotic stromal cells induced by locally produced IL-4 is involved in the development of endometriosis.
- concentration decreased from periodontal health to disease
- There is a lack of association or interactions between the IL-4, IL-4Ralpha and IL-13 genes, and rheumatoid arthritis.
- IL-4 exerts profibrotic effects by activating hIHFs and inducing collagen production and secretion
- HBD-2 and HD-5 may be involved in defending against invasion by BV-related microorganisms and the decrease in IL-4 concentration may increase susceptibility to bacterial vaginosis
- dynamic interplay between IL-4 and IL-21 in regulating the production of IgG subclasses and IgA
- there was a marked trend for an association between the IL-4 -590 (C>T) SNP and pre-eclampsia (chi(2)= 5.87, P = 0.055), with a prevalence of TT homozygous women in this group (OR 4.455, 95% CI 1.286-15.350).
- that the IL-4 haplotype tagged by the -589T allele reduces the risk of MI in young individuals
- The follicular lymphoma microenvironment contains increased levels of IL-4, with prominent tumor basal phosphorylation of Erk.
- There was no significant association of IL-4 and IL-6 gene polymorphisms with the risk of prostate cancer, however, an IL-6 gene variant may be associated with prostate progression and bone metastasis.
- the RANTES G-403A allele increased the relative risk for cerebral infarction in the subjects without the IL-4 VNTR allele 3
- IL-4 induces PTP1B mRNA expression in a phosphatidylinositol 3-kinase-dependent manner and enhances PTP1B protein stability to suppress IL-4-induced STAT6 signaling.
- combination IL-2/IL-4 inhibits GCRalpha nuclear translocation in human T cells, and this effect is reversed by IFN-gamma via inhibition of p38 MAPK activation.
- Haplotype T(-590)/T(-33)/allele 2 VNTR of IL-4 gene was significantly more frequent in CP patients. The 3 polymorphisms in IL-4 gene act in a cooperative fashion and suggest the high-production IL-4 haplotype was associated with an increased risk for CP.
- Th2 cell cytokine IL-4 decisively determines the phenotype of ongoing immune responses by orchestrating the functional phenotype of newly immigrating DC precursors.
- Study of pathways engaged in transgenic model of thymic stromal lymphopoietin-driven airway inflammation demonstrates that simultaneous blockade of interleukin (IL)-4 and IL-13 can reverse established asthma-like chronic airway disease.
- interleukin-4 and amphiregulin have roles in the proliferation of human airway smooth muscle cells and cytokine release
- CCL11 induces SOCS1 and SOCS3 expression in murine macrophages, human monocytes, and dendritic cells and inhibits GM-CSF-mediated STAT5 activation and IL-4-induced STAT6 activation in a range of hematopoietic cells.
- findings were helpful to understand the long clinical observation and revealed that endogenous IL-4, the product of host immune response, can be used by tumor cells to facilitate their growth
- House dust mite-sensitive DCs exposed to Der p 1 downregulated IDO activity and tipped the T(H)1/T(H)2 cytokine balance toward IL-4, resulting in sustainable IDO suppression.
- Pre-treatment with IL-4 and IL-13, but not IFN-gamma, induced desensitization of the human airway smooth muscle cells to IL-13 as measured by eotaxin secretion and calcium transients to histamine.
- Expanded catalog of genetic loci implicated in psoriasis susceptibility.