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Validated All-in-One™ qPCR Primer for IL2RA(NM_000417.2) Search again
Product ID:
HQP101019
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD25, IDDM10, IL2R, IMD41, TCGFR, p55
Gene Description:
interleukin 2 receptor subunit alpha
Target Gene Accession:
NM_000417.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis.
Gene References into function
- Here we show, in four cell lines of human adult T cell lymphoma/leukemia origin, that the three IL-2R subunits are compartmented together with HLA glycoproteins and CD48 molecules in the plasma membrane., IL-2Ralpha (CD25) was also found in DRMs
- the mechanics of this complex receptor system and to other receptor complexes in the immune system that send signals by clustering receptor subunits.
- Secretion levels of sIL-2R, IL-6, IL-8, IL-10, and TNF-alpha, but not IL-4 and IL-12, are elevated in activated T-cells in large granular lymphocytic leukemia associated with autoimmune disorders.
- Cells from all infected patients but from none of the controls responded specifically to ST-Ag by expressing surface CD25 on culture
- Elevated IL-2 and IL-2 receptor serum levels correlated with the severity of cutaneous graft-versus-host disease after bone marrow transplantation, and evidence suggested that both act together in the development of cutaneous GVHD.
- Increased CD25 expression, especially in CD4(+) T cells but also in CD8(+) T cells, without increases in expression of the beta and gamma chains of the IL-2 receptor was detected in HIV patients following long-term intermittent interleukin 2 therapy
- investigation of the presence of IL-2alpha, and IL-2beta receptors on fresh and isolated sperm
- Serum soluble interleukin-2 receptor correlates with probable etiology and severity of disease in eosinophilia.
- Anti-apoptotic signaling by the interleukin-2 receptor reveals a function for cytoplasmic tyrosine residues within the common gamma (gamma c) receptor subunit.
- Expression of cd25 on the surface of activated T lymphocytes are, in part, responsible for apoptosis of T lymphocytes and excessive turnover of immune cells in the circulation of patients with breast cancer.
- Total expression level of membrane IL-2R in peripheral blood monocytes before and after being stimulated with PHA was lower than those in normal controls.
- The development of breast tumour is associated with an increased expression of IL-2 receptor alpha and this expression also seems to be associated with the malignancy of the tumour.
- Mucosal induction of tolerance against dietary antigens is associated with the development of CD4(+)CD25(+) T cells.
- IL-2R alpha, IL-15R alpha, IL-2/15R beta and gamma(c)-subunits, as well as MHC class I and II glycoproteins formed supramolecular receptor clusters in lipid rafts of the T lymphoma line.
- Decreased levels of CD25 expression occur in T-cell lymphoma when it involves the lymph nodes.
- interleukin-2 receptor has a role in diverse types of cancer [review]
- Antagonists may minimize need for steroids in kidney transplantation. (review)
- Soluble interleukin 2 receptor alone is not a good candidate marker in the diagnosis of pancreatic cancer; it can, however, be used in association with carbohydrate antigen 19-9 for this purpose
- decreased production of IL-2sRa by peripheral blood mononuclear cells after induction by non-sialylated serotype HS-3 C. jejuni serotypes
- The presence of CD4+CD25+ (regulatory T cells in head and neck cancer patients might be, in part, responsible for downregulation of antitumour immune responses.
- CD4(+)CD25(+) fetal T cells are present in the human fetus from week 14 onwards, which shows that generation of regulatory-suppressor T cells is consubstantial to the generation of a functional and self-tolerant immune system
- We found strong statistical evidence in the case-control collection (P=6.5x10(-8)) for a T1D locus in the CD25 region of chromosome 10p15 and replicated the association in the family collection
- human leukocyte antigen-DR and CD25 may have a role in progression of cutaneous lupus erythematosus
- Lipid rafts are the functional microdomains for IL-2alpha receptors.
- Phenotypic studies demonstrated decreased frequency of CD4+CD25+ T cells in patients with chronic graft-versus-host disease
- model of the ternary complex of interleukin-10 with its soluble receptors
- Analysis of biopsies from cardiac allograft recipients during acute rejection by quantitative (Q)-PCR. FOXP3 mRNA expression was restricted to the CD25population that inhibited the proliferation of allostimulated CD25 cells.
- CD25-expressing memory CD8+ T cells in elderly persons appear to be a prerequisite for intact immune responsiveness in the absence of naive T cells in old age.
- A quantitative relationship is established between receptor alpha subunit binding affinity and mitogenic activity for IL-2 and IL-15.
- over-expression of ITF2B protein inhibited the expression of IL-2Ralpha gene in Jurkat cells in an NRE-dependent manner
- The soluble receptor balance (sIL-2R/sIL-4R) in patients with severe hemolytic uremic syndrome may shift, depending on the disease state of the patients
- the crystal structure, at 2.3 A resolution, of the quaternary complex of IL-2 with the extracellular domains of receptors IL-2R alpha, IL-2Rss, and gamma c.
- Increased expression of interleukin-10 and transforming growth factor-beta1 mRNA was also detected in patients with TB but did not correlate with regulatory T-cell markers.
- The coexpression of CD25 and the costimulatory molecule CD134 on memory T-cells provides a novel marker for type 1 diabetes-associated T-cell immunity.
- Increase of functional CD25 in T cells in cancer patients is a response to the process of malignant transformation.
- sIL-2R and IL-6, but not IL-2, IL-4, or IL-10, may have a role in preventing progression of aggressive non-Hodgkin's lymphoma
- euthyroid female relatives of autoimmune thyroid disease patients had a reduced expression of CD25 on CD4+ T cells
- CD4 and CD25 in T cells, which are increased in breast cancer, are decreased after vaccination with a HER2/neu peptide (E75) and GM-CSF vaccine
- during chronic lung allograft rejection there was a significant decline of T(IL-10) cells with concomitant expansion of collagen type V-specific IFN-gammaproducing Th1 cells
- STAT5b propagates an important IL-2-mediated signal for the in vivo accumulation of functional regulatory T cells
- CD4+ CD45RO+ Foxp3+ CD25hi T lymphocytes are highly proliferative, with a doubling time of 8 days, compared with memory CD4+ CD45RO+ Foxp3- CD25- (24 days).
- data suggest that despite normal levels of IL-10R expression, and an apparent lack of abnormalities in intracellular signals induced through this receptor, immune cells from SLE patients exhibit aberrant pattern of gene expression induced through IL-10R
- percentage of activated T cells expressing CD25 is lower in decidua than in peripheral blood but the opposite in regulatory T cells.
- These data support studies using in vitro generated and expanded human CD4+ CD25+ regulatory T cells with indirect allospecificity as therapeutic reagents to promote donor-specific transplantation tolerance.
- IL-2Ralpha solely has a prognostic value and should be considered as a major prognostic factor for adult lymphoblastic leukemia.
- In conclusion, numbers of circulating immunosuppressive T(reg) are reduced in transplant recipients.
- Extracorporeal photopheresis may may modulate peripheral Treg cell number containing cd25 antigens after lung transplantation
- In children with Down's syndrome and periodontitis, serum IL-2 receptor levels were significantly higher than in control subjects
- CD25(bright+) T cells suppressed the proliferation of CD25(-/dim) donor- and third party-stimulated responder T cells in renal allograft patients.
- although CD4+ CD25+ T regulatory cells induced by IL-10 seem to contribute to aspects of sepsis-induced lymphoid immune suppression, the oblation of CD25+ cells does not provide a survival advantage or disadvantage
- The levels of peripheral CD4(+)CD25(high) T cells in recipients and donors could be helpful for predicting of the onset and outcome of acute graft-versus-host disease.
- study found statistical evidence of an association between Graves' disease and the CD25 region (P = 4.5 x 10(-4)), with the pattern of association of the 20 tag SNPs similar to that found in type 1 diabetes
- Hepatocellular carcinoma cell supernatants increase expansion and function of IL2RA of regulatory T-cells.
- implicate Interleukin-10-producing CD25(-)Foxp3(-) T cells in the pathogenesis of human visceral leishmaniasis
- IL2RA is a promising candidate gene for type 1 diabetes, suggesting a potential role of IL2Ralpha in the pathogenesis of type 1 diabetes, likely involving regulatory T-cells.
- No evidence of IL2RA/CD25 polymorphisms contribute to multiple sclerosis.
- These results suggest that Tregs inhibit osteoclast differentiation from PBMCs in a cytokine-dependent manner, not by cell-to-cell contact manner and that TGF-beta and IL-4 may be the key cytokines for this suppressive function of Tregs.
- the expression of the IL-2R is relevant to human plasmacytoid dendritic cells (PDC) activation, and that IL-2 may be an important auto- and/or paracrine factor modulating the activation and survival of PDC
- Human CD4(+)CD25(bright) T cells play a major role in tolerating conceptus antigens and therefore may contribute to the maintenance of pregnancy.
- CD4(+)CD25(+)IL-7Ralpha(+) cell population represents a specific marker to monitor allospecific CD4 T cell responses in blood and in tissues after organ transplantation.
- The expression of CD25-alpha at the cell surface was increased by DHA, SA, and PA but was unaffected by EPA, OA, and LA.
- reveal a novel role for non-Hodgkin lymphoma B cells in the development of intratumoral regulatory T cells
- Impaired function of CD4+/CD25+ T regulatory lymphocytes characterizes the self-limited hepatitis A virus infection.
- Alleles of IL2RA and IL7RA and those in the HLA locus are identified as heritable risk factors for multiple sclerosis.
- CD25 expression on T cells was significantly increased for subjects ingesting Echinacea at 24 h with notable increases in activation from Astragalus and Glycyrrhiza
- Association of IL2RA type 1 diabetes (T1D) susceptibility genotypes with lower circulating levels of the soluble IL-2RA suggest that an inherited lower immune responsiveness predisposes to T1D.
- CD4+CD25+CTLA-4+FoxP3+ T cells have roles in patients with systemic lupus erythematosus and are impaired after conventional treatments
- demonstrate the capacity of a CD25-directed immunotoxin to selectively mediate a transient partial reduction in circulating and tumor-infiltrating T regulatory cells in vivo
- A decrease in CD4+CD25+ T cell numbers in mitral stenosis patients might suggest a role for cellular autoimmunity in a smoldering rheumatic process.
- CD4+CD25highFoxp3+ T cells have a role in squamous cell carcinoma of the head and neck
- Fusion of interleukin 2 (IL2) to the N terminus of R336A-aerolysin results in a hybrid that has little or no activity against cells that do not have an IL2 receptor.
- Results demonstrate variable expression of IL-2 and its receptor in various types of lung tumours, but no simple relationship could be detected between tissue expression of the markers and proliferative activity.
- Cyclosporine therapy after kidney transplant resulted in a reduction of cd25 regulatory T cells.
- Immunoreactivity for CD25 in gastrointestinal mucosal mast cells is specific for systemic mastocytosis and can be used to confirm the diagnosis.
- Suggest that CD4+CD25+bright T cells were likely to play anti-inflammatory and immunosuppressive roles in the pathogenesis of primary Sjogren's syndrome.
- CD25 in cutaneous mast cells may be useful for stratifying adult patients presenting with Urticaria pigmentosa for additional clinical evaluation
- These data suggest that dual recruitment of FcgammaRIIIa and the IL-12R to lipid raft microdomains allows for enhanced activation of downstream signaling events that lead to IFN-gamma production.
- Moderate intensity long-term training may increase the recruitment of active memory CD4+CD25+ in men rather than women.
- Campath-1H (Alemtuzumab) is an effective immunodepletion agent used in renal transplantation which increass CD25 regulatory T cells.
- We here confirm clustering of IL2Ralpha and IL15Ralpha at the submicron scale. Our single-molecule data reveal that a non-negligible percentage of the receptors are organized as monomers.
- IL2RA and IL7RA genes confer susceptibility for multiple sclerosis in two independent European populations.
- Increased numbers of CD4+CD25high Tregs may influence IgG4 production in autoimmune pancreatitis.
- during HIV infection, specific changes in the fraction of CD4(+) T cells expressing CD25 and/or CD127 are associated with disease progression
- Results showed that patients with chronic periodontitis presented increased frequency of T lymphocytes and CD4+CD25+ T cells in the inflammatory infiltrate of gingival tissues.
- IRF-1 is a key negative regulator of CD4(+)CD25(+) Treg cells through direct repression of Foxp3 expression
- acquisition of suppressive behavior by activated CD4(+)CD25(-) T cells requires the expression of CTLA-4, a feature that appears to be facilitated by, but is not dependent on, expression of FoxP3
- Genetic variation in IL-2 and CD25 regions may promote T1D susceptibility by influencing transcription and/or splicing and hence IL-2 and CD25 expression at the protein level in different immune cell subsets.
- Evidence is provided that the increase of CD4+CD25 high T cells and FoxP3 transcripts is associated with operational tolerance in liver transplanted patients during immunosuppression withdrawal.
- results provide strong evidence that the IL2RA/CD25 gene represents a juvenile idiopathic arthritis susceptibility locus
- allelic heterogeneity" at the IL2RA region between Multiple sclerosis and type 1 diabetes.
- Results describe the association of IL-2 receptor alpha with MS and reveal a genetic heterogeneity of the associated polymorphisms and risk alleles between MS and T1D suggesting different immunopathological roles of IL2RA in these two diseases.