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Validated All-in-One™ qPCR Primer for HLA-E(NM_005516.5) Search again
Product ID:
HQP100197
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HLA-6.2, QA1
Gene Description:
major histocompatibility complex, class I, E
Target Gene Accession:
NM_005516.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
HLA-E belongs to the HLA class I heavy chain paralogues.
Gene References into function
- Peptide binding specificity of HLA-E.
- Signal peptide processing by signal peptidase is essential in the generation of HLA-E-binding epitopes derived from the signal sequence of polymorphic MHC class I molecules.
- Soluble HLA-E tetramers refolded with different peptides (specifically stained K14 cells. HLA-E tetramer binding was reduced by pretreatment with anti-CD94 mAb alone, but was completely prevented in combination with anti-clonotypic mAb.
- expresssion modulates cytokine production of monocyte generated dendritic cells
- HLA-E allelic variants: differential expression, peptide affinities, crystal structures, and thermal stabilities.
- HLA-E can present a peptide derived from the signal sequence of human hsp60.
- Recognition of mycobacterium tuberculosis- derived antigen being presented in the context of HLA-E.
- Expression was detected in tumor cell lines showing an imbalance in heavy chain/beta(2)m expression, particularly in tumor cell lines with alterations in the expression of heavy-chain genes.
- HLA-E has been found in all cells of the placenta that express either the membrane-bound or soluble form of HLA-G, consistent with HLA-E being complexed with the HLA-G signal sequence-derived nonamer in these cells.
- Results suggest that the GATA-1 transcription factor represents a cell type-restricted mediator of interferon-gamma induction of the HLA-E gene.
- The presence of the appropriate HLA class I alleles with leader sequence-derived peptides and HLA-E heavy chain may not be sufficient to allow HLA-E surface expression in tumor cell lines as opposed to lymphoid cells.
- the T cell epitope HCV core 35-44 stabilizes HLA-A2 and HLA-E and inhibits cytolysis mediated by natural killer cells
- up-regulation of HLA-E expression and reduced susceptibility to NK cell cytotoxicity in HIV-1 infection
- An HLA-E single chain trimer inhibits human NK cell reactivity towards porcine cells.
- Data show that short tandem repeat of exon 5 of MHC class-I chain related gene A and association with nasopharyngeal carcinoma in a southern Chinese population.
- Potential role of human leukocyte antigen (HLA)-E polymorphism on the incidence of early infections in patients identicslly matched for class I and Ii antigens.
- Generation of an inhibitory HLA-E epitope derived from multidrug resistance-associated protein 7 (MRP-7) provides insight into the immunoregulatory role of HLA-E during cell stress.
- HLA-E-restricted cytotoxic T-lymphocytes (CTL) may represent an additional effector cell type involved in defenses against human cytomegalovirus, a virus which escapes the control exerted by conventional CTL or natural killer (NK) cells.
- The results of this analysis confirmed several previously reported coding sequence variants, identified several new allelic variants, and also defined extensive variation in intron and flanking sequences.
- HLA-E expression and shedding are normal features of melanocytes, which are conserved in melanoma cells of primary tumors, but become dependent on IFN-gamma induction after metastasis
- strong positive directional selection is acting for maintaining the observed low polymorphism on HLA-E, -F and -G loci
- The homozygous state for HLA-E*0103 allele behaves as a protective genetic factor against acute graft versus host disease and transplant-related mortality.
- Variant HLA-E and HLA-G molecules appear to function independently and synergistically, increasing the risk of Behcet's disease.
- indicate that the SNPs of the inhibitory receptor CD94/NKG2A and its haplotypes, as well as its ligand HLA-E, are associated with Behcet's disease immune systems
- summary of the current knowledge on HLA-E and HLA-G expression, regulation and functional relevance in various malignancies [review]
- A bi-allelic polymorphism (Arg107Gly) of human leukocyte antigen-E (HLA-E) locus was investigated in sickle cell anemia patients originating from sub-Saharan Africa. HLA-E*0101/E*0101 genotype was more frequent among the group with severe infections.
- the invariant CD94 chain plays a more dominant role in interacting with HLA-E in comparison to the variable NKG2 chain.
- An extensive substitution analysis throughout the entire region of HLA-E led to the identification of nine amino acid positions that are significantly involved in the cell surface expression of HLA-E molecules.
- Upregulation of HLA-E could be a marker of shorter disease-free survival in Dukes' C patients.
- crystal structure of CD94-NKG2A in complex with HLA-E bound to a peptide derived from the leader sequence of HLA-G
- The evolution of the NKG2x/CD94 family of receptors has likely been shaped both by the need to bind the invariant HLA-E ligand and the need to avoid subversion by pathogen-derived decoys.
- HLA-E overexpression seemed to be associated with invasive cervical cancer and HPV16/18 infection.
- findings show HLA-E is a good, and not a poor, beta(2)microglobulin assembler, and TAP/tapasin-assisted ligand donation is only one, and possibly not even the major, pathway leading to its stabilization and surface expression
- this is the first description of population frequencies of nine different SNPs in HLA-E in three main large ethnic groups