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Validated All-in-One™ qPCR Primer for HDAC2(NM_001527.3) Search again
Product ID:
HQP100084
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HD2, KDAC2, RPD3, YAF1
Gene Description:
histone deacetylase 2
Target Gene Accession:
NM_001527.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq].
Gene References into function
- mSin3A interacts with HDAC2 via the HDAC-interacting domain (HID) of mSin3A.
- Phosphatase inhibition leads to histone deacetylases 1 and 2 phosphorylation and disruption of corepressor interactions
- Regulation by protein kinase CK2
- Data show that Daxx associates with proteins critical for transcriptional repression, such as histone deacetylase 2 and Dek, a chromatin-associated protein reported to change the topology of DNA in chromatin in vitro.
- HDAC2 is phosphorylated by CK2 and recruited by Sp1 or Sp3
- The MLL repression domain specifically interacts with HDAC2.
- IkappaBalpha regulates the transcriptional activity of homeodomain-containing proteins positively through cytoplasmic sequestration of HDAC1 and HDAC3
- HDAC2 binds to HoxA10 and has a role in repressing gene transcription in undifferentiated myeloid cells
- interaction of HPV type 31 E7 with HDACs and the integrity of the zinc finger-like motifs are essential for extending the life span of keratinocytes and for stable maintenance of viral genomes
- Increased HDAC2 expression is associated with colon cancer
- PELP1 recruits HDAC2 and masks histones using two separate domains
- involved in the early events of carcinogenesis, so candidate marker for tumor progression, target for cancer therapy
- GIOT-1-mediated transrepression was recovered by down-regulation of HDAC2 expression with small interfering RNA of HDAC2
- A rationale for targeting HIF-1 alpha with histone dacetylase inhibitors against class II isozymes as anticancer agents in renal carcinoma cells.
- Brg1 and HDAC2 have roles in GR trans-repression of the pituitary POMC gene and misexpression in Cushing disease
- Transgenic HDAC2 regulates expression of many fetal cardiac isoforms, including glycogen synthase kinase 3 beta.
- High expression of HDAC2 is associated with cancer tissues
- unmodified HDAC2 is associated with the coding region of transcribed genes, whereas phosphorylated HDAC2 is primarily recruited to promoters
- CK2 may be a key mediator for HDAC1 and HDAC2 activation under hypoxia in tumor cells
- we showed that the known effects of HDACs on differentiation and proliferation of cancer cells observed in vitro can also be confirmed in vivo. The class I HDAC isoforms 1, 2 and 3 are differentially expressed in prostate cancer.
- HDAC2 mutations have a role in human tumorigenesis through the derepression of key genes from multiple cellular transformation pathways
- depletion of HDAC2 results in simultaneous depletion of ER and PR, and potentiates the effects of antihormonal therapy in ER-positive cells
- Strong prognostic impact of HDAC isoforms in colorectal cancer.
- LPA enhances survival of cancer cells by increasing HDAC1 and HDAC2 activity and reducing histone acetylation
- High expression of HDAC2 is more common in aggressive than indolent cutaneous T-cell lymphoma.
- Recruitment of HDAC-1 and -2 by TNF-alpha, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells.
- Frequent HDAC2 mutations are found in MSI tumors and HDAC2 plays a major role in mediating apoptotic response to HDAC inhibitors through direct regulation of APAF1.
- In HCT116 cells, HDAC2 was shown to localize to the APAF1 promoter, and siRNA-mediated down-regulation of HDAC2 induced APAF1 expression, suggesting HDAC2 directly represses APAF1 expression.
- class I histone deacetylase (HDACs), specifically HDAC2, and the transcriptional repressor Snail play a central role in the suppression of 15-hydroxyprostaglandin dehydrogenase expression.
- P/CAF deacetylation by HDAC3 and in a minor degree by HDAC1, HDAC2, or HDAC4 leads to cytoplasmic accumulation of P/CAF.