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Validated All-in-One™ qPCR Primer for SLCO1B3(NM_019844.3) Search again
Product ID:
HQP099030
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HBLRR, LST-2, LST-3TM13, LST3, OATP-8, OATP1B3, OATP8, SLC21A8
Gene Description:
solute carrier organic anion transporter family member 1B3
Target Gene Accession:
NM_019844.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Human organic anion transporting polypeptide 8 promoter is transactivated by the farnesoid X receptor/bile acid receptor.
- HNF3beta represses transcription of the OATP8 but not the OATP-C gene, providing a mechanism for reduced expression of OATP8 in hepatocellular carcinoma
- The functional consequences of three polymorphisms and one artificial mutation include differences in the localization and in hepatocellular transport of several OATP1B3 proteins.
- OATP1B3 play important roles in CDCA uptake into the liver.
- the transcription of the LST-2 gene is regulated by three transcription factors, FXR, HNF1alpha, and HNF3beta.
- although both OATP-C/1B1 and OATP8/1B3 are highly expressed, and able to transport bile acids, their mechanisms of action are different
- these results indicate that OATP1B3/OATP-8 and OATP1B1/OATP-C most likely function as bidirectional facilitated diffusion transporters and that GSH is not a substrate or activator of their transport activity.
- Bosentan uptake into Chinese hamster ovary cells expressing human OATP1B1 or OATP1B3, and was efficiently inhibited by cyclosporin A, rifampicin, and to a lesser extent by sildenafil.
- LST-2 overexpression is associated with a hormone-dependent growth mechanism of the breast cancer
- Performed transport assays in OATP1B3-expressing cells as a method of indentifying novel antineoplastic agents.
- A set of double transfectants expressing OATP1B3 combined with OAT1B1 can be used as a tool for rapid identification of hepatic uptake and efflux transporters of many organic anions that are substrates of OATP1B1 or OATP1B3.
- SNPs in and SLCO1B3 may predict the risk of leukopenia/neutropenia induced by docetaxel chemotherapy.
- Alterations of OATP1BB3 function by oral antidiabetic drugs have to be considered as potential mechanisms underlying drug-drug interactions.
- Pregnane X receptor ligands, by inhibiting or stimulating OATP1B3-mediated uptake, can lead to drug-drug interactions at the transporter level.
- The common SLCO1B3 GG/AA haplotype is associated with impaired testosterone transport and improved survival in patients with prostatic cancer.
- A polymorphism in a transporter, in SLCO1B3, that increases testosterone import is associated with a shorter time to androgen independence in patients with prostate cancer who are treated with ADT.
- identificaction of three amino acid residues (Y537, S545, and T550) in TM10 of organic anion transporting polypeptide 1B3 that are important for cholecystokinin octapeptide transport.
- OATP1B3 overexpression in colorectal cancer cells may provide a survival advantage by altering p53-dependent pathways.