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Validated All-in-One™ qPCR Primer for GNAI2(NM_002070.3) Search again
Product ID:
HQP098971
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
GIP, GNAI2B, HG1C, H_LUCA15.1, H_LUCA16.1
Gene Description:
G protein subunit alpha i2
Target Gene Accession:
NM_002070.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- CXCL12 upregulated T-cell activation, and an alpha(i) G-coupled protein mediated signaling pathway was necessary for stimulation of T cells by CXCL12.
- Modulation of G(ialpha(2)) signaling by the axonal guidance molecule UNC5H2
- valproic acid-induced expression of Galphai2 is regulated by Sp1 transcription factor
- Cholesterol lowering by pravastatin might increase the response of the heart to parasympathetic stimulation and changes in Galpha(i2) expression might serve as a molecular marker for this effect
- Galphai2 has a role in calcium-sensing receptor-mediated ERK1/2 activation
- Abnormal regulation of GNAI2 was studied in cultured skin fibroblasts from insulin-resistant hypertensive individuals.
- in elderly subjects of similar ages, those with diabetes have 1.7-fold higher levels of Galpha(i2) and twofold higher levels of Gbeta(1).
- the Galphai2 gene promoter is transcriptionally activated through nuclear factor-kappaB and antioxidant response elements
- a GNAI2 -318 C>G SNP impairs transcriptional activity through specific binding of Sp1 and is associated with high SBP in Caucasians from Italy
- These results suggest that Galphai2 is involved specifically in the activation of TRPC4.
- These results show that G(i2) protein is involved in D(2)R-mediated ERK activation but beta-arrestins 1 and 2 are either not involved or play minor role.