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Validated All-in-One™ qPCR Primer for GCLM(NM_002061.3) Search again
Product ID:
HQP098867
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
GLCLR
Gene Description:
glutamate-cysteine ligase modifier subunit
Target Gene Accession:
NM_002061.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit.
Gene References into function
- Polymorphism in the 5'-flanking region is associated with myocardial infarction
- A -588T polymorphism of the GCLM gene causes a decrease in endothelial NO bioactivity, causing impairment of endothelium-dependent vasomotor function in large and resistance coronary arteries. The GCL-GSH-NO axis may defend against coronary artery diseas
- GCLM has a role in cisplatin (CDDP)-resistance in lung cancer
- Review. The most important element in both Gclc and Gclm expression is the electrophile response element in their promoters.
- Reduction-oxidation regulation of gamma-GCSh expression is mediated by p38 mitogen-activated protein kinase signaling.
- in most tissues, glutanmate-cysteine ligase modifier submit (GCLM) is limiting, suggesting an increase in GCLM alone would increase gamma-GC synthesis; in kidney, gamma-GC synthesis may be controlled post-translationally
- The gene of the key GSH-synthesizing enzyme, glutamate cysteine ligase modifier (GCLM) subunit, is strongly associated with schizophrenia in two case-control studies and in one family study.
- upregulation of gamma-glutamate-cysteine ligase as part of the long-term adaptation process to iron accumulation in neurons
- the present data strongly indicates that, in spite of its functional impact, the GCLM C-588T polymorphism is - at least in a German cohort - not an independent risk indicator of coronary artery disease and myocardial infarction
- Results suggest that a common genetic variation in the GCLM gene might not contribute to the risk of methamphetamine-use disorder and schizophrenia in the Japanese population.