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Validated All-in-One™ qPCR Primer for GAS6(NM_000820.3) Search again
Product ID:
HQP098341
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AXLLG, AXSF
Gene Description:
growth arrest specific 6
Target Gene Accession:
NM_000820.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis.
Gene References into function
- Gas6-deficient mice are protected against thrombosis, indicating that a physiological function of Gas6 is that of a procoagulant. This effect is mediated, at least in part, through platelets, as Gas6-deficient platelets are less prone to aggregation.
- Data suggest that Gas6 and Axl signal transduction is aberrantly stimulated in endometriotic endometria, and is plausibly related to its growth potential.
- GAS6 has a V-shaped arrangement of laminin G-like LG domains strengthened by an interdomain calcium-binding site
- Axl and Gas6 expression might be involved in childhood thyroid tumorigenesis around Chernobyl.
- Gas6 sustains fetal oligodendrocyte viability by receptor activation and downstream signaling via the phosphatidylinositol 3-kinase-dependent/ proto-oncogene protein akt pathway.
- Gas6, acting as a growth factor, is increased in the process of kidney allograft dysfunction and in chronic allograft nephropathy.
- GAS6/Axl signaling is involved in human renal disease.
- GAS6 intron-exon structure, identification of single nucleotide polymorphisms and possible association with stroke.
- New information about the mechanism underlying Gas6 protection from apoptosis in primary endothelial cell cultures.
- Gas6/Axl pathway could play a role in the complex events taking place during the early changes of ovarian cancer progression.
- Axl stimulation by GAS6 results in inhibition of the ligand-dependent activation of vascular endothelial growth factor (VEGF) receptor 2 and the consequent activation of an angiogenic program in vascular endothelial cells
- Axl overexpression and activation by Gas6 could be involved in progression of prostate neoplastic disease
- This report demonstrates that Gas6-induced downregulation of Axl is blocked by inhibitors of endocytosis and lysosomal degradation, but not by inhibitors of proteosomal activity.
- The crystal structure at 3.3 A resolution of a minimal human Gas6/Axl complex reveals an assembly of 2:2 stoichiometry.
- Restoration of Gas6 mRNA by statins was mediated by mRNA stabilization, and not by an increase in transcriptional activity.
- We conclude that gas6 signaling through the Axl receptor and the PI3 kinase/Akt1 survival pathway protects oligodendrocytes from growth factor withdrawal and TNFalpha-mediated cell death.
- Gas6 has a role in aspirin resistance
- the CACA haplotype, is less prevalent in patients with stroke
- GAS6 signals via its receptor tyrosine kinases and plays a key role in the perpetuation of platelet aggregates and clot retraction.
- Axl and Gas6 are frequently overexpressed in both glioma and vascular cells and predict poor prognosis in GBM patients
- adiponectin antagonizes the stimulatory effect of TNFalpha on vascular calcification by restoration of the AMPK-dependent Gas6-mediated survival pathway.
- Gas6-promoted phagocytosis was inhibited by L-type Ca(2+)-channel blockage, which in turn may be activated by integrin receptor stimulation.
- These findings identify a role for Gas6 in plaque composition and stability.
- gas6 protects endothelial cells from apoptosis by a mechanism that involves PI3K-Akt-dependent inactivation of FOXO1a.
- The experiments described in this paper propose a novel functional role of gamma-carboxylation in gas6-mediated endothelial cell survival.
- Found no association between plasma Gas6 levels and coronary artery disease.