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Validated All-in-One™ qPCR Primer for GAPDH(NM_001289745.2) Search again
Product ID:
HQP098077
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
G3PD, GAPD, HEL-S-162eP
Gene Description:
glyceraldehyde-3-phosphate dehydrogenase
Target Gene Accession:
NM_001289745.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
Gene References into function
- We identified a nuclear high molecular weight (HMW) GAPDH species in Huntington's disease cells, suggesting a connection between nuclear GAPDH function and huntingtin localization in this CAG expansion neuronal disease.
- GAPDH is not an ideal internal standard for specific gene expression in testicular tissue specimens
- GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders--REVIEW
- dissociation of the GAPDH protein from the HMW species restores its enzymatic activity.
- HIF-2alpha regulates glyceraldehyde-3-phosphate dehydrogenase gene expression in vascular endothelial cells.
- Transcription factors bound to glutamine-responsive element in GAPDH promoter are C/EBPalpha and -delta.
- The p38/gapdh component of OCA-S binds directly to OCT1.
- Glycoaldehyde inactivates GAPD.
- Glyceraldehyde-3-phosphate dehydrogenase might be a novel target for vaccinia anti-apoptotic modulation.
- nuclear GAPDH has a role in the maintenance and/or protection of telomeres
- Thioredoxin regulates the expression of GAPDH.
- GAPDH mRNA expression was correlated with evidence of tumor progression in thymoma.
- data suggest that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) imparts a unique function necessary for membrane trafficking from vesicular tubular clusters (VTCs) that does not require GAPDH glycolytic activity
- significant association between late-onset Alzheimer's disease and a compound genotype of the three GAPD genes
- Subcellular interactions may mitigate oxidative stress-induced GAPDH modification in human aging.
- results demonstrate that glyceraldehyde-3-phosphate dehydrogenase, a glycolytic and microtubule binding protein, co-localized to neurofibrillary tangles and immunoprecipitated with paired helical filament-tau
- Marked variability of GAPDH expression between tissue types which establish comparative levels of expression and can be used to add value to gene expression data in which GAPDH is used as the internal control.
- GAPDH modified by 4-hydroxy-2-nonenal and 4-hydroxy-2-hexenal is degraded by a giant serine protease, releasing the 23-kDa fragment, not by proteasome or TPP I
- evidence that GAPDH is an epithelial binding receptor for Porphyromonas gingivalis fimbriae
- Conditions associated with elevated intracellular methylglyoxal could modify GAPDH activity in vivo.
- GAPDH is an integral part of the sarcolemmal K(ATP)-channel protein complex, where it couples glycolysis with the K(ATP)-channel activity.
- Data show that the insertion of ferriprotoporphyrin IX (FP) into the red cell membranes exerts two opposite effects on membrane bound G3PD.
- Amyloid-beta induces disulfide bonding and aggregation of GAPDH in Alzheimer's disease
- GAPDH might be involved in cell cycle regulation by modulating cyclin B-cdk1 activity.
- GAPDH is inaccurate to normalize mRNA levels in studies investigating the effect of bisphosphonates on gene expression; this gene could be considered a potential target to observe the effects of bisphosphonates on cancer cells
- OxLDL downregulated GAPDH via a H2O2-dependent decrease in protein stability. GAPDH protein damage resulted in marked depletion of cellular ATP levels.
- The GAPD gene and its pseudogene may play a role in the development of late-onset Alzheimer disease. However, the effect, if any, is likely to be limited.
- TPI and GAPDH may be candidate Ags for an autoimmune response to neurons and axons in multiple sclerosis.
- identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a new interacting partner of TPPP/p25 within the alpha-synuclein positive Lewy body
- GAPDH interacts with transferrin and the GAPDH-transferrin complex is subsequently internalized into the early endosomes.
- A decrease in the dehydrogenase activity of GAPDH was noted in Alzheimer disease.
- alpha-crystallin B, glyceraldehyde phosphate dehydrogenase (GAPDH), and alpha-enolase identified as significantly S-glutathionylated in Alzheimer's disease infeior parietal lobule
- Nitric oxide-mediated S-nitrosylation of GAPDH and subsequent nuclear translocation of GAPDH might function as a mediator of TRAIL-induced cell death in thyroid cancer cells.
- Glyceraldehyde-3-phosphate dehydrogenase enhances transcriptional activity of androgen receptor in prostate cancer cells
- Study observed no hypoxia-induced regulation of GAPDH expression in the 3 glioblastoma cell lines; also, GAPDH expression was similar in patient tumor samples of low-grade astrocytoma and glioblastoma, suggesting a lack of hypoxic regulation in vivo.
- These results suggest that the polyQ domain, but not the polyP domain, plays a role in the sequestration of GAPDH to aggregates by mutant htt
- Overexpression of human GAPDH in Escherichia coli did not enhance mutagenesis by diepoxybutane.
- Succination of GAPDH and other thiol proteins may provide the chemical link between glucotoxicity and the pathogenesis of diabetic complications.
- Results show that nuclear GAPDH is acetylated at Lys 160 by the acetyltransferase p300/CREB binding protein (CBP) through direct protein interaction, which in turn stimulates the acetylation and catalytic activity of p300/CBP.
- A redox-modulated direct p38/GAPDH-Oct-1 interaction nucleates the occupancy of the H2B promoter by the OCA-S complex, in which p36/LDH plays a critical role in the hierarchical organization of the complex.
- GAPDH, a multifunctional protein, now adds regulation of mRNA stability to its repertoire.
- the nuclear translocation of GAPDH during oxidative stress constitutes a protective mechanism to safeguard the genome by preventing structural inactivation of APE1.
- Involvement of GAPDH in the X-ray resistance of HeLa cells is reported.
- U6snRNA, GAPDH mRNA and CPEB1 mRNA levels may be useful as tumor markers for genital cancers.
- Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.