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Validated All-in-One™ qPCR Primer for ALDH1A1(NM_000689.4) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq].
Gene References into function
- Effect of gene on proliferation arrest in a non-small cell bronchopulmonary cancer line
- The promoter region of a gene can influence gene expression, the ALDH1A1 promoter regions were studied to identify polymorphism, to assess their functional significance, and to determine whether they were associated with a risk for developing alcoholism.
- ALDH1 is strongly and specifically expressed in human mesencephalic dopamine neurons. Low levels of ALDH1 expression correlate with dopamine neuron dysfunction in the two investigated human conditions.
- ALDH1A1*2 may be associated with protection from the development of alcohol and other substance use disorders.
- Results demonstrate that aldehyde dehydrogenase is a key regulator of hematopoietic stem cell differentiation.
- Random mutagenesis followed by a filter-based screening assay has been used to identify a mutant of human class 1 aldehyde dehydrogenase (ALDH1) that was no longer inhibited by Mg(2+) ions but was activated in their presence.
- ALDH1A1 appears to be the major if not the only enzyme responsible for the oxidation of 3-deoxyglucosone to 2-keto-3-deoxygluconate.
- ALDH1 can be a dermal biomarker for atopic dermatitis disease.
- promoter polymorphism ALDH1A1*2 may be associated with increased risk for the development of alcohol dependence in Indo-Trinidadians
- Retinoic-acid-induced RAR-CAK signaling events appear to proceed intrinsically during granulocytic development of normal primitive hematopoietic cells. ALDH-governed RA availability may mediate this process by initiating RAR-CAK signaling.
- Overexpression of ALDH1A1 inhibits lymphopoiesis and promotes myelopoiesis in murine hematopoietic progenitors to alter cell fate and induce tumor growth
- findings show differential expression of aldehyde dehydrogenase 1 between fibroids & myometrium is maintained in cell culture (without endothelial cells), & that this gene is differentially regulated by retinoids in myometrial compared with fibroid cells
- ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome.
- analysis of bioactivation of nitroglycerin by purified mitochondrial and cytosolic aldehyde dehydrogenases ALDH1 and ALDH2
- ALDH, expressed along CD133, can more specifically characterize the tumorigenic liver cancer stem cells population.
- Down-regulation of aldehyde dehydrogenase 1A1 by siRNA transfection reveals its involvement in promoting and inhibiting pancreatic cancer cell lines.
- In summary, we have identified a molecular signature that distinguishes NF1-PA from S-PA and found that ALDH1L1 underexpression is associated with aggressive histology and/or biologic behavior