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Validated All-in-One™ qPCR Primer for EPOR(NM_000121.3) Search again
Product ID:
HQP095536
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EPO-R
Gene Description:
erythropoietin receptor
Target Gene Accession:
NM_000121.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. [provided by RefSeq].
Gene References into function
- Amino acid determinants of beta-hairpin conformation in erythropoeitin receptor agonist peptides derived from a phage display library
- While both Epo and Epo-R were detected in all samples of isolated epithelial cells analysed throughout the menstrual cycle, neither one was detected in isolated stromal cells. Epo and Epo-R protein expression in glandular epithelial cells was increased
- REVIEW: Role of erythropoietin receptor in myelodysplastic syndrome and leukemia.
- The extracellular binding site for ERP is now characterized. The site is located in the membrane proximal, extracellular part of the receptor. ERP binds to a region on the EPOR that contains the same sequence as ERP
- evidence for pY429pY431 being a new high affinity binding site for SOCS-3 on the EpoR
- functional significance of expression in breast cancer
- JAK2 activation mediates cross-talk between extracellular domain mutants of the beta-subunit of the GM-CSF receptor and EpoR
- Epo and Epo-R localized within glandular epithelial cells in both peritoneal endometriosis and eutopic endometrium. Epo-R expression lower in black peritoneal lesions.
- Expression of erythropoietin receptor splice variants in tumor cell lines.
- Majority of papillary thyroid carcinomas (PTC) from children and adolescents express EPO-R, a finding associated with favorable prognostic indicators and a lower risk of recurrence.
- Increased erythropoietin receptor expression is associated with advanced-stage disease, lymphovascular invasion, lymph node metastasis of endometrial carcinoma
- erythropoietin receptor and nitric oxide production are stimulated by erythropoietin and hypoxia in vascular endothelial cells
- These data demonstrate that EPO can promote differentiation of neuronal stem cells into astrocytes in an EPO receptor dependent manner, and this effect may be associated with the activation of ERK kinase and NF-kappaB pathway.
- activated Epo receptors appear to be quickly degraded after ubiquitination by 2 proteolytic systems that proceed successively
- Epression of erythropoietin receptor in prostate cancer cell lines suggesting that these cells may serve as useful experimental models for further studies of erythropoietin receptor function for growth regulation in prostate cancer.
- the erythropoietin-receptor pathway modulates survival of cancer cells
- The Erythropoietin Receptor mutations have been shown to cause the myeloproliferative disorders disease.
- detailed mapping of interactions of several signalling proteins with phosphorylated tyrosine-containing motifs in the cytosolic domain OF EPOR
- the TM-JM junction of EpoR forms an N-terminal helix cap required for function
- erythropoietin and EPOR are co-expressed in tumor cells, suggesting that erythropoietin may potentially function as an autocrine or paracrine factor in head and neck cancer
- erythropoietin receptor was expressed in almost all samples in non-small cell lung carcinomas.
- Results showed that VHL disease-associated renal clear cell carcinomas and renal cysts coexpress and erythropoietin receptor and erythropoietin.
- Coexpression of Erythropoietin and Erythropoietin receptor was found in all five Hippel Lindau disease-associated pheochromocytomas.
- the erythropoietin functions that promote cell survival and proliferation are affected by aluminum exposure through mechanisms involving erythropoietin receptor
- Y285 and Y344 in the cytoplasmic domain of EPOR-ME may contribute to increased Epo sensitivity that is characteristic of primary familial and congenital polycythemia phenotype.
- CaM binds to the membrane-proximal EpoR cytoplasmic region and plays an essential role in activation of Jak2-mediated EpoR signaling
- data demonstrate that transformed, non-malignant and malignant prostate epithelial cells have functional EpoR
- Erythropoietin and erythropoietin receptor are expressed in head and neck squamous cell carcinoma
- No EPOR exon VIII mutations were found in members of 8 Greek families with primary familial and congenital polycythemia.
- Coexpression of erythropoietin and its receptor plays a important role in tumorigenesis of sporadic clear cell renal cell carcinoma.
- findings show that three thyroid cancer cell lines expressed Epo and EpoR mRNA
- Collectively, the results suggest that Jak2 is the sole direct signaling molecule downstream of EpoR required for biological activity.
- erythropoietin receptor is not ubiquitinated following erythropoietin stimulation in this cancer cell line, and there is no turnover of the receptor in either unstimulated or stimulated cells
- Doubt concerning the significance of previous immunohistochemical studies of EPOR expression in malignancy and emphasize the need for more specific anti-EPOR antibodies to define the true extent of EPOR expression in neoplastic tissue.
- The EpoR expression correlated significantly with apoptosis and correlated significantly with tumor size and was significantly associated with the presence of lymphovascular space involvement in Cervical cancers.
- The Epo receptor may be important for secretion, endothelial progenitor cell mobilization, and angiogenesis in ischemic tissue as well as Epo in peripheral vasculature of EpoR-deficient-rescued transgenic mice, compared with wild type.
- Recognition of a vascular EpoR system which is endowed with significant pathophysiological functions opens new exploitation horizons in cardiovascular medicine.
- upregulation of EpoR in temporal cortical and hippocampal astrocytes is an early, potentially neuroprotective, event in the pathogenesis of sporadic Alzheimer disease.
- A novel sporadic EPOR 1453G->A (Trp439Stop) mutation was detected. All familial erythrocytosis cases, varied in phenotype, presented the EPOR 1414C->G (Tyr426Stop) mutation.
- Five of the antibodies recognized recombinant rat and human EPOR in HEK293 cells by Western blotting, but the same antibodies yielded different and inconsistent results when using human UT-7 cells or rat brain tissue.
- Statistically analyzed distributions of the proteins reflected functional dependences among STAT3, HIF-1alpha, EPO and EPOR in cellular signal conduction.
- Upregulation of EPOR is not uncommon for PCa and upregulated EPOR in high-grade PIN suggests upregulation of EPOR is an early event for prostate carcinogenesis.
- Epo and EpoR were expressed neuroblastomas but did not modify tumor cell proliferation.
- erythropoietin receptor and erythropoietin have roles in the autocrine/paracrine mechanism of growth enhancement in human ovarian cancer cells
- These results suggest that the erythropoietin-independent EpoR-signaling pathway plays a potential role in cell proliferation and angiogenesis in human pterygium.
- we provide evidence that expression of the erythropoietin receptor is a common feature of malignant mesothelioma cells cultured in vitro
- The EPO-R cytosolic domain is involved in its exit.Sequence motifs that participate in endocytosis can also modulate transport along the secretory pathway.
- HLA-G5 downregulates EPOR constitutive signaling of JAK2 V617F-expressing erythroleukemia cells, inhibiting cell proliferation via G1 cell cycle arrest.
- EpoR juxtamembrane regulatory motif essential for Epo-dependent JAK2 activation is not essential for the activation of JAK2V617F.
- Erythropoietin receptor is present in normal parathyroid, parathyroid adenoma, and hyperplasia.
- EPO and EPO Receptor have roles in apoptosis in pancreatic cells
- Epo overexpression is an early event in the retina of diabetic patients, not associated with any change in EpoR.
- we have found no evidence that EpoR is overexpressed in tumours or gets to the surface of tumour cells.
- activation of the EpoR gene by Wt1 may represent an important mechanism in normal hematopoiesis
- expression in placenta increased in abortive tissue and hydatidiform mole
- new mutations in the erythropoietin receptor gene may have a role in pure erythrocytosis
- ETV6/RUNX1 leads to EPOR up-regulation and that activation by EPO might be of relevance to the biology of ETV6/RUNX1-positive acute lymphoblastic leukemias
- These findings suggest that EpoR over-expression and activation in breast cancer cells has the potential to contribute to tumor progression by promoting the proliferation and invasiveness of the neoplastic cells