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Validated All-in-One™ qPCR Primer for S1PR1(NM_001400.4) Search again
Product ID:
HQP095014
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD363, CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, S1P1
Gene Description:
sphingosine-1-phosphate receptor 1
Target Gene Accession:
NM_001400.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion.
Gene References into function
- in mouse, this protein is essential for vascular maturation
- phosphorylation of the sphingosine 1-phosphate (SSP) receptor "endothelial differentiation gene 1" (EDG1 or S1P(1)) receptor is increased in response to either SSP or phorbol 12-myristate 13-acetate (PMA) exposure but not lysophosphatidic acid
- functions as an intracellular signaling molecule and angiogenesis factor
- activates Rho family G proteins and cell motility
- S1P(1) inhibits Ca(2+) signalling most likely via G(i) proteins and classical PKC isoforms. Co-expression of S1P(1) with S1P(3), but not S1P(2), reversed the inhibitory effect of S1P(1), furthermore suggesting a specific interplay of S1P receptor subtype
- S1P1 receptors expressed in mouse Th1 cells mediate suppression of T cell proliferation and cytokine production.
- Immunosuppressant FTY720 activates sphingosine 1-phosphate receptors that affect responsiveness of lymphocytes to chemokines such as stromal cell-derived factor 1.
- cross-communication between the prototypical barrier-protective S1P and barrier-disruptive PAR1 pathway
- Persistent expression of S1P1 receptor by lymphocytes of S1P1 receptor-transgenic mice suppresses delayed-type hypersensitivity and results in production of significantly more IgE antibody (Ab) and less IgG2 Ab than in wild-type mice.
- endothelial protein C receptor ligation and sphingosine 1-phosphate receptor transactivation results in endothelial cell cytoskeletal rearrangement and barrier protection through activated protein C
- Signaling by S1P1 receptor-transgenic mice affects systemic trafficking of peripheral T cells and primary immune responses; levels of S1P1 receptor expression represent an important mechanism of immune regulation.
- Tyrosine sulfation of S1P1 may be a major controller of S1P effects on T cell traffic
- S1P-induced recruitment of S1P1 to CEM fractions promotes PI3 kinase-mediated Tiam1/Rac1 activation required for alpha-actinin-1/4-regulated cortical actin rearrangement and EC barrier enhancement
- The constitutively active endogenous S1P1 receptor enhances PDGF-induced cell migration.
- heterotrimeric G protein-dependent ERK1/2 activation is mediated by IGF-1 and IGF-2 by transactivating sphingosine 1-phosphate receptors
- Transactivation of sphingosine 1-phosphate receptors is essential for vascular barrier regulation
- FTY720-P targets the S1P1 receptor to the ubiquitinylation and proteasomal degradation pathway
- Sphingosine 1-phosphate-induced inflammatory response genes expression is mediated through S1P(1) and S1P(3)
- FTY720 induces time-dependent modulation of S1P receptors on human OPCs with consequent functional responses that are directly relevant for the remyelination process.
- S1P(1) receptor, without stimulating [Ca(2+)](i) increases, mediates chemotaxis of keratinocytes
- S1P-mediated signaling via the S1P1/Gi/Rho GTPases pathway activates integrin alpha v beta 3, which is indispensable for S1P-stimulated chemotactic response of ECs.
- S1P promotes lymphangiogenesis by stimulating S1P1/G(i)/phospholipase C/Ca(2+) signaling pathways.
- HDL has endothelial barrier promoting activities, which are attributable to its S1P component and signaling through the S1P1/Akt pathway.
- Datas suggest that filamin A links sphingosine kinase 1 and sphingosine-1-phosphate receptor 1 to locally influence the dynamics of actin cytoskeletal structures at lamellipodia to promote cell movement.
- Plays essential roles in the pathogenesis of rheumatoid arthritis by modulating fibroblast-like synoviocyte migration, cytokine/chemokine production, and cell survival.
- FOXO1 controls the expression of L-selectin and EDG1 and EDG6, receptors that regulate lymphocyte trafficking
- subconfluent pulmonary artery smooth muscle cells express predominantly S1P2 and S1P3 receptors; S1P1 receptor mRNA levels are significantly up-regulated following bFGF treatment
- the crucial role of activation of the SPHK1S1PS1P1 signaling pathway in response to inflammatory mediators in endothelial cells in regulating endothelial barrier homeostasis.