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Validated All-in-One™ qPCR Primer for AFP(NM_001134.2) Search again
Product ID:
HQP094782
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AFPD, FETA, HPAFP
Gene Description:
alpha fetoprotein
Target Gene Accession:
NM_001134.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life.
Gene References into function
- In this unified theory of cancer, it is suggested that AFP is commonly secreted by cancers and by binding to 67 kD AFP receptors, confers a malignant phenotype to tumors and immunosuppresses the host.
- AFP by binding to a 67 kD AFP receptor can block apoptosis in normal cells and cancer cells. It may be a rare example of a soluble inhibitor of apoptosis.
- p53 and HNF-3 bind the promoter of AFP in a mutually exclusive manner, repressing and activating the gene respectively.
- blood levels of the neoplasm protein can be used to monitor hepatoblastoma response to therapy and modify treatment
- both the ratio of AFP-L3 (AFP-L3%) and the absolute value of AFP-L3 (AFP-L3-AV) were examined in 80 patients with HCC, and evaluated with respect to characteristics of HCC such as grade of differentiation, size of tumor and morphological findings
- Repression of alpha-fetoprotein gene expression under hypoxic conditions in human hepatoma cells: characterization of a negative hypoxia response element that mediates opposite effects of hypoxia inducible factor-1 and c-Myc.
- Variant forms of transcripts expressed in human hematopoietic progenitors; Implications for their developmental potential towards endoderm
- A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor.
- Nkx2.8 bound to the active AFP promoter, and antisense inhibition of Nkx2.8 mRNA translation selectively reduced expression of both the endogenous human AFP gene and transfected reporters containing the rat AFP promoter.
- alpha-fetoprotein directly binds to CCR5 in primary macrophages
- The alpha-fetoprotein gene is expressed in cytotrophoblasts from early human placentas.
- APF is fucosylated in hepatoma cells after treatment with acyclic retinoid
- alpha-fetoprotein regulates neoplastic growth through the presence of an alpha-fetoprotein cell surface receptor (review)
- Degree of alterations in maternal serum hCG and alpha-fetoprotein levels varied between fresh and frozen-thawed embryos and also between mode of fertilization.
- positively regulates cytochrome c/dATP-mediated apoptosome complex formation
- AFP is a diagnostic but rather insensitive tissue marker for hepatocellular carcinoma
- AFP may act as a specific proangiogenic factor of endothelial cells within the fetomaternal unit during advanced stages in pregnancy
- mouse and human alpha-fetoprotein enhancers are strikingly different
- AFP can stimulate the expression of some oncogenes to enhance the proliferation of human hepatocellular carcinoma Bel 7402 cells.
- AFP induces dysfunction and apoptosis of APCs, offering a mechanism by which hepatocellular carcinoma escapes immunological control.
- This first identification of a mutation in the AFP gene demonstrates for the first time that deficiency of AFP is compatible with human normal fetal development and further reproduction in males.
- AFP elevation appears to contribute to vascular invasion and hepatocellular carcinoma progression
- AFP is able to promote the expression of FasL and TRAIL in hepatoma cells and enhance the expression of Fas and TRAILR in lymphocytes
- alpha-FP and SCCA yielded a correct serologic diagnosis in 90.83% of the hepatocarcinoma patients.
- AFP mRNA is a more reliable marker of metastasis compared to serum AFP
- Transient co-transfection of p53 family members showed that p53 and transactivating (TA)-p73, but not TA-p63, repress endogenous AFP transcription additively or independently.
- The gene expression profile of hepatoblasts throughout life consists of high levels of expression of alpha-fetoprotein(AFP).
- We show here that alpha-fetoprotein cancels XIAP-mediated inhibition of endogenous active caspases in cytosolic lysates of tumor cells, as well as XIAP-induced blockage of active recombinant caspase 3 in a reconstituted cell-free system.
- We demonstrated that foldability of the AFP molecule from its denatured-reduced state is independent of its starting source, the presence or absence of glycosylation and fatty acids, and the refolding method used (dialysis or dilution).
- mRNA may be a predictive value for recurrence of hepatocellular carcinoma in living donor liver transplantation.
- AFP mRNA-expressing cells may be a useful predictor of hepatocellular carcinoma recurrence in liver transplant patients.
- These results indicate that C/EBPalpha regulates AFP gene expression through direct binding to multiple sites in the human AFP gene in cultured human cells.
- describe the case of a 44-year-old woman who presented with vaginal bleeding, pelvic mass, and preoperative elevated AFP serum level
- effects of a necrosis-inducing treatment (embolization) on anti-alpha-fetoprotein (AFP)-specific CD4(+) T cell responses in hepatocellular carcinoma (HCC) patients and controls using an array of AFP-derived peptides.
- The positive rate of AFP mRNA in liver transplantation patients is higher than that at preoperation, a difference that is statistically significant
- The novel polymorphisms identified in the promoter region of the AFP gene may be pathologically significant in HCC.
- AFP increase in patients with liver cancer was positively correlated with the infection of HBV and HCV. The serum AFP elevation by the infection of HBV and HCV is one of mechanisms which lead to hepatocarcinogenesis
- Mesenchymal hamartoma of the liver reported with features of Beckwith-Wiedemann syndrome and high AFP levels.
- Results show that measurements of AFP, CEA and CA125 are more readily affected by long-term frozen storage compared with frequent freezing-thawing.
- These data support the idea that ZHX2 contributes to AFP repression in the liver after birth and may also be involved in AFP reactivation in liver cancer.
- EpCAM and alpha-fetoprotein are expressed in hepatocellular carcinoma
- frequency of AFP(46-55) CD4(+) T cells is significantly higher (p = 001) in patients with hepatocellular carcinoma than in healthy donors
- serum a-fetoprotein may have a similar role in intrahepatic cholangiocarcinoma and hepatocellular carcinoma
- Results indicate that the poor prognosis associated with high alpha-fetoprotein in hepatocellular carcinoma is due to high cell proliferation, high angiogenesis, and low apoptosis.
- The N-glycan structures of the Lens culinaris agglutinin (LCA)-reactive fraction of alpha-fetoprotein (AFP-L3), a tumor marker of hepatocellular carcinomas (HCC), were analyzed.
- targeting of AFP with siRNA could be a potential therapeutic approach for hepatoma
- substitution of aa 70 of HCV-1b core region is an important predictor of elevated AFP in non-HCC (hepatocellular carcinoma) patients.
- In hepatocellular carcinoma, pre-transplant AFP levels >400 ng/ml were associated with higher tumor recurrence.
- Alpha-fetoprotein and human chorionic gonadotropin beta are elevated in high-grade neuroendocrine tumors with a rapidly progressive course and poorer survival.
- Serum samples were investigated for carcinoembryonic antigen (CEA), CA125 and MUC1, alpha-foetoprotein, neuron-specific enolase and CA19.9.
- results suggest that AFP may positively regulate cell proliferation by enhancing the apoptosis resistance via dysfunction of the p53/Bax/cytochrome c/caspase-3 signaling pathway in AFP
- Elevated alpha-fetoprotein is associated with primary central nervous system germ cell tumors.
- In patients with hepatocellular carcinoma, a high frequency of AFP-specific CD8(+) T cells directed against different epitopes suggest that AFP has a strong and broad immunogenicity.
- Molecular background of AFP in liver cancer cells as revealed by global RNA expression analysis is reported.