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Validated All-in-One™ qPCR Primer for SLC22A12(NM_144585.3) Search again
Product ID:
HQP092039
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
OAT4L, RST, UAT, URAT1, hURAT1
Gene Description:
solute carrier family 22 member 12
Target Gene Accession:
NM_144585.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a urate transporter and urate-anion exchanger which regulates the level of urate in the blood. This protein is an integral membrane protein primarily found in kidney. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- Molecular identification of a renal urate anion exchanger that regulates blood urate levels
- PDZK1 plays a role in regulating the functional activity of URAT1-mediated urate transport in the apical membrane of renal proximal tubules.
- SLC22A12 is a major gene for hypouricemia but not hyperuricemia in Japanese.
- heterozygous mutations of the URAT1 gene (Q297X and IVS2+1G>A) may be recurrent mutations of the URAT1 gene in a Japanese population
- G774A mutation in SCL22A12 gene serves as a suppressing factor for development of gout. Mutation decreased uric acid levels.
- A single nucleotide polymorphism (SNP) in the urate transporter gene SLC22CA12 was found to be associated with elevated serum uric acid levels
- Report of patients with heterozygous and homozygous mutations in the hURAT1 gene in a family with renal hypouricemia associated with exercise-induced acute renal failure.
- Functioning as an antiporter, hURAT1 mediates the uptake of urate from the lumen into proximal tubule cells in exchange for organic and inorganic anions.
- This study was undertaken to elucidate whether SLC22A12 gene mutations are responsible for low serum uric acid levels in Greek people. No previously reported mutation of URAT1 was associated with primary renal hypouricaemia in Greek subjects.
- is a urate anion exchanger regulating blood urate levels and proposed to be involved in the multimolecular complex "transportsome" that allows the cooperation of multiple transporters.
- The G774A mutation in the SLC22A12 gene encoding URAT1 ( urate anion exchanger 1 ) predominates in Japanese renal hypouricemia.
- losartan inhibited URAT1 and thereby it lowered Sur levels in hypertensive patients.
- The in vivo role of GLUT9 is supported by the fact that a renal hypouricemia patient without any mutations in SLC22A12 was found to have a missense mutation in SLC2A9, which reduced urate transport activity in vitro.
- polymorphism of the SLC22A12 gene may be involved in renal urate handling and the concentration of serum uric acid
- Prevalence of hypouricaemia and SLC22A12 mutations in healthy Korean subjects.