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Validated All-in-One™ qPCR Primer for POSTN(NM_006475.2) Search again
Product ID:
HQP090837
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
OSF-2, OSF2, PDLPOSTN, PN
Gene Description:
periostin
Target Gene Accession:
NM_006475.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility.
- Data suggest that periostin-mediated angiogenesis derives in part from the up-regulation of the vascular endothelial growth factor receptor Flk-1/KDR by endothelial cells through an integrin alpha(v)beta(3)-focal adhesion kinase signaling pathway.
- Over expression of Periostin promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway
- Downregulation of periostin is associated with higher grade bladder cancer
- Expression of OSF-2 is likely to play a role in the pathogenesis of nasopharyngeal carcinoma.
- periostin has an active role in the epithelial-mesenchymal transformation and metastasis that requires cross-talk between integrin and EGFR signaling pathways
- Although a major effect of the SNPs in the POSTN geneson breast cancer susceptibility and prognosis was excluded, the effect of the POSTN C-33G SNP on prognosis needs further characterization.
- periostin is a gene highly overexpressed in breast cancer cells and is correlated with poor outcome in a cohort of poor prognosis estrogen receptor-positive tumours.
- These findings suggest an important role of periostin in pancreatic cancer.
- Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer
- Periostin was overexpressed in gastric cancer and lymph node metastasis, which suggests that periostin plays an important role in the progression and metastasis of gastric cancer.
- identifies both stromal and melanoma cells as sources of periostin production and correlates POSTN expression levels with increased primary tumor thickness and metastatic process development
- loss of WT periostin by down-regulation and/or alternative splicing, which produces Variant I, is closely correlated with the development of bladder cancer.
- Periostin protein expression was abundant in the infarct border of human hearts with acute myocardial infarction (AMI), and it is essential for cardiac healing after AMI.
- The expression of periostin/OSF-2 is regulated by ovarian steroid hormones in rat uterus and human endometrium.
- induced expression of periostin (to 150 ng/ml) altered the morphology of cancer cells, changing them from mesenchymal to epithelial phenotypes with the induction of epithelial markers and a reduction of mesenchymal markers
- Periostin is secreted by pericryptal and cancer-associated fibroblasts in the colon, both of which support the growth of epithelial components
- periostin is a member of a vitamin K-dependent gamma-carboxylated protein family characterized by the presence of fasciclin domains; carboxylated periostin is produced by bone-derived mesenchymal cells and is found in mineralized bone nodules in vitro
- Periostin deposits in the stroma of invasive and intraductal neoplasms of the pancreas.
- Periostin is a novel autocrine mitogen secreted by mural epithelial cells with the potential to accelerate cyst growth and promote interstitial remodeling in autosomal dominant polycystic kidney disease.
- periostin has a role in tumor progression and a worse prognosis in NSCLC, as well as with angiogenesis and lymphangiogenesis
- Overexpression of recombinant human periostin in rat cardiomyocytes and fibroblasts did not affect mitosis, DNA synthesis, or cell cycle.