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Validated All-in-One™ qPCR Primer for XRCC5(NM_021141.3) Search again
Product ID:
HQP088690
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
KARP-1, KARP1, KU80, KUB2, Ku86, NFIV
Gene Description:
X-ray repair cross complementing 5
Target Gene Accession:
NM_021141.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Gene References into function
- A functional interaction of Ku with Werner exonuclease facilitates digestion of DNA containing 8-oxoadenine and 8-oxoguanine modifications.
- transcription initiates from a CpG island and is induced by p53 through a nearby p53 response element
- Ku80 alteration is unlikely to be involved in multiple myeloma
- Expression of Ku70/Ku80 proteins is decreased in malignant melanomas of the oral cavity.
- Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair.
- Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus
- role of expression in NF-kappaB activation and COX-2 expression
- Ku associates with hTERT, and this interaction may function to regulate the access of telomerase to telomeric DNA ends
- These results suggest that association of Ku with transcription sites is important for maintenance of global transcription levels.
- The expression of Ku is upregulated in human neuroectodermal tumor cells by retroviral DNA integration
- Transcripts of Ku70 and Ku86 genes were detected by RT-PCR and Ku protein was localized in the nucleus of neutrophils as a heterodimer
- The mechanism that regulates for nuclear localization of Ku70 and Ku80 appears to play, at least in part, a key role in regulating the physiological function of Ku in vivo.
- Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment
- DNA repair proteins Ku70 and Ku80 expression is lost in cell nucleus after oxidative stress
- frequency of flat or raised moles is associated with polymorphism at or near this DNA repair gene and certain alleles are associated with less efficient DNA repair and greater nevus density
- binding of the Ku complex at chromosomal breaks may be necessary to maintain the sliding clamps (PCNA) on chromatin
- Results show that Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) modulate RAG-mediated cleavage during V(D)J recombination.
- DNA-binding component of human OF-1 (which binds Herpes simplex virus type 1 origin of replication) contains Ku70 and Ku80 proteins
- DNA degradation diminished the amount of Ku70 associated with gamma-H2AX but not that of nuclear DNA helicase II.
- Ku is involved in transcriptional recycling with androgen dependent promoters
- Ku70-binding site of Ku80 is required for the stabilization of Ku70 in the cytoplasm and for the nuclear translocation of Ku80 through its heterodimerization with Ku70.
- Ku70/80 interacts directly with the RNA component of human telomerase, independent of the human telomerase reverse transcriptase protein.
- Study using HCT116 haplo-insufficient cells and Orc2 hypomorphic cells demonstrates that human Ku80 and Orc2 bind to replication origins independently of each other, while Ku binding precedes that of Orc-3, -4, and -6.
- findings suggest Ku80 as a cellular factor targeted by Tax in engendering clastogenic DNA damage.
- The present study suggests that Ku binds IRES -(internal ribosomal entry site)elements within RNA molecules, and that Ku plays a role in the modulation of IRES mediated mRNA translation.
- The pre-steady state binding of Ku with various DNA duplex substrates was studied and a redox-sensitive Ku-DNA interaction was identified.
- Dimeric particles are observed in which two DNA-PKcs/Ku70/Ku80 holoenzymes interact through the N-terminal HEAT repeats.
- Functions in nonhomologous DNA end joining.
- The three-dimensional structure of the human full-length Ku70-Ku80 dimer at 25 A resolution, alone and in complex with DNA, by using single-particle electron microscopy was reconstructed.
- poly(ADP-ribose)polymerase-1 and Ku70/Ku80 are transcriptional regulators of S100A9 gene expression
- the gene encoding Ku86 (XRCC5) is an essential gene in human somatic cells, and its absence cannot be suppressed by the loss of p53 function
- low mRNA and protein expression in the BRCA1/BRCA2 and XRCC5 genes occur in lung adenocarcinoma and squamous cell carcinoma, respectively, and promoter hypermethylation is the predominant mechanism in deregulation of these genes
- These data support a new role for Ku in the migration of monocytes into tissues, which depends on a tightly regulated pathway of intracellular redistribution.
- Results describe the effect of Ku80 on both provirus integration and the resulting transgene expression in cells transduced with retroviral vectors.
- The expression and subcellular localization of Ku80 in the nucleus demonstrated the involvement of this protein in the photo-oxidative cell death pathway.
- potential relevance of Bin1-Ku interaction to cancer are discussed in light of these findings
- Variation in DNA repair genes XRCC3, XRCC4, XRCC5 and susceptibility to myeloma.
- Although the different genotypes were not associated with the risk of bladder cancer, individuals not carrying the two tandem repeats allele had an increased risk of bladder cancer compared with those carrying the allele with two repeats.
- HCT116 cells heterozygous for deficiency have high rates of chromosomal instability.
- Expression of Ku86 in breast cancer tissue and normal breast tissue with immunohistochemistry are largely equal.
- These data suggest that Ku80 functions as a novel receptor for Tbeta4 and mediates its intracellular activity.
- Ku80 is essential for Ku70 accumulation at DSBs. Three domains of Ku80, i.e., the N-terminal alpha/beta, the DNA-binding, and Ku70-binding domains, seem to necessary for the accumulation at or recognition of DSBs in the early stage after irradiation
- The data suggest the interplay between the DNA-replication and cell-cycle machineries and shed light on a new role of Ku in G1-S transition.
- Our data demonstrate that in a bladder tumour cell line up-regulation of Ku end-binding activity without any marked change in Ku expression underlie radiation resistance.
- Hence Ku80 may serve as a promising therapeutic target, particularly for recurrent esophageal tumors.
- Pretreatment determination of DNA-PK/Ku86 activities might be helpful in identifying patients who will actually benefit from platinum-based treatment.
- Hypoxia downregulates Ku70/80 expression in cervical carcinoma tumors.
- ApoC-IV overexpression may perturb lipid metabolism leading to lipid accumulation. HCV core protein may modulate ApoC-IV expression through Ku antigen and PPARgamma/RXRalpha complex.
- Results describe the response of BRCA1 at DNA double-strand breaks produced by laser microirradiation, and show that accumulation of the BRCA1 N terminus, but not the C terminus, at DSBs depended on Ku80.