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Validated All-in-One™ qPCR Primer for MMP13(NM_002427.3) Search again
Product ID:
HQP088639
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CLG3, MANDP1, MDST, MMP-13
Gene Description:
matrix metallopeptidase 13
Target Gene Accession:
NM_002427.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Gene References into function
- AGRE site plays a rate-limiting role in human collagenase-3 production.
- Investigation of the role of Endo180/urokinase-type plasminogen activator receptor-associated protein as a collagenase 3 (matrix metalloproteinase 13) receptor
- involvement of MAPKs, AP-1 and NF-kappa B transcription factors in the IL-1 induction of MMPs in chondrocytes
- Reduction of cytokine-induced expression and activity of MMP-1 and MMP-13 by mechanical strain in MH7A rheumatoid synovial cells
- REVIEW: The structure, regulation, and function of human matrix metalloproteinase-13
- Data show that keratinocytes use an alternative plasminogen and matrix metalloproteinase-13-dependent pathway for dissolution of collagen fibrils.
- induction by transforming growth factor-beta via activation of p38 mitogen-activated protein kinase pathway
- Two new MMP13 promoter polymorphisms. Genotype for one MMP13 polymorphism associated with fibrous plaque in black males. MMP13 expressed in all layers of aorta. Polymorphism a functional variant. Genetic risk factor for extent of fibrous plaque.
- translational repression by an alternatively spliced form of T-cell-restricted intracellular antigen-related protein
- MMP13 expression is regulated by IGF-1 and OP-1
- induction of the MMP-13 gene by TNF-alpha is mediated by ERK, p38, and JNK MAP kinases as well as AP-1 and NF-kappaB transcription factors
- Together with other prognostic markers, determination of MMP-13 in ascitic fluid may help to identify patients at risk for early death and help to individualize adjuvant therapy
- catabolic role of ET-1 in osteoarthritis cartilage via MMP-1 and MMP-13 up-regulation.
- MMP-13, uPA, and PAI-1 antigen levels were determined in the synovium of patients with osteoarthritis
- MMP13 is upregulated in breast cancer
- MMP-1 and MMP-3 secretion by gastric epithelial cells are differentially regulated by E prostaglandins and MAPKs
- gene expression regulation in Lyme disease
- regulation of the PLC pathway through the PTH1R is increased by elevating expression of G(11)alpha in osteoblastic cells leading to increased PTH stimulation of MMP-13 expression by increased stimulation of AP-1 factors c-jun and c-fos.
- study showed a marked expression of MMP-13 in dental pulp tissue of both sound and carious teeth
- MMP-9 and MMP-13 are involved in stroke progression and the neuroinflammatory response
- Down-regulated by integrin alphavbeta6, not essential for the degradation of type I collagen by oral squamous cell carcinoma cells.
- GADD45beta plays an essential role during chondrocyte terminal differentiation and mediates MMP-13 gene expression
- activation of MMP-13 as well as MMP-9 induced by H-Ras is involved in angiogenesis and with farnesyl transferase inhibitors, in part, exerts their anticancer effects
- MMP-13 appears to be a factor associated with tumor aggressiveness in cutaneous malignant melanoma
- MMP-13 can degrade members from two classes of small leucine-rich repeat proteoglycans. Site at which biglycan is cleaved by MMP-13. MMP-13 induced SLRP degradation may represent early event affecting collagen network by exposing MMP-13 cleavage site.
- the involvement of p38 MAP kinase in the hyaluronan oligosaccharide induction of MMP-13
- The expression of matrix-modeling genes in chronic idiopathic myelofibrosis (cIMF) is not influenced by the JAK2 mutation status but is predominantly related to the stage of disease.
- a novel role for human MMP-13 in regulating dermal fibroblast survival, proliferation, and interaction in 3D collagen.
- Since both S100A4 and RAGE are up-regulated in osteoarthritis cartilage, this signaling pathway could contribute to cartilage degradation in this disease.
- TUNEL-positive cells and MMP-3- and -13-expressing cells were distributed in the degenerative articular cartilage and reparative fibrocartilage tissue in osteochondritis dissecans (OCD) of the elbow.
- Premature induction of hypertrophy-related molecules (type X collagen and matrix metalloproteinase 13) occurred before production of type II collagen and was followed by up-regulation of alkaline phosphatase activity.
- No convincing evidence was found to support the association of MMP13 SNPs with increased breast cancer risk or survival.
- activity in gingival crevicular fluid samples was significantly increased in active sites from progressive periodontal disease, supporting its role in the alveolar bone loss
- These results identify an unexpectedly broad involvement for p8 in key cellular events linked to cardiomyocyte hypertrophy and cardiac fibroblast matrix metalloproteases production, both of which occur in heart failure.
- nicotine stimulates bone matrix turnover by increasing production of tPA and MMP-1, 2, 3, and 13, thereby tipping the balance between bone matrix formation and resorption toward the latter process
- We have solved the 2.0 A crystal structure of the complex between the catalytic domain of human MMP-13 (cdMMP-13) and bovine TIMP-2.
- oncostatin M-stimulated ADAMTS-4 and MMP-13 expression is mediated by extracellular signal-regulated kinases, Janus kinase 3/STAT1/3 and phosphatidylinositol 3-kinase/Akt and by cross talk between these pathways
- deregulation of cross-talk between mitogen-activated protein kinases and protein kinase Cdelta signaling may contribute to the etiology of osteoarthritis in human patients
- CpG-ODN-treatment also increased MMP-13 activity and neutralizing anti-MMP-13 antibody prevented CpG-ODN-induced invasion in TLR9(+) CaP cells.
- Increased production of reactive oxygen species but not nitric oxide as secondary messengers in the chondrocyte fibronectin fragment signaling pathway leads to increased production of MMPs, including MMP-13.
- MMP-13 is involved in osteogenic differentiation.
- a matrix metalloprotease-13 inhibitor reduces cartilage damage in vivo without joint fibroplasia side effects
- Nucleolin interacts with the AP-1 site within the promoter sequence of the metalloproteinase-13 gene.
- TauCl differentially inhibits the expression of MMP-1 and MMP-13 in human synoviocytes
- BFGF activates the MAPK and NFkappaB pathways that converge on ELK1 to control production of MMP13 by articular chondrocytes.
- MMP-13 may serve as a marker for progression of malignant peripheral nerve sheath tumors.
- Y-box binding protein-1 (YB-1) as a new repressor of MMP-13 transactivation.
- Fibroblasts of the interface from aseptically loosened endoprostheses selectively express MMP-13
- ROCK-II is a critical mediator of colon cancer cell invasion through its modulation of MMP-2 and -13 at the site of invadopodia but regulates proliferation in non-malignant intestinal cells.
- MyD88, IRAK1 and TRAF6 proteins are crucial early mediators for the IL-1-induced MMP-13 regulation through MAPK pathways and AP-1 activity.
- the MMP-13 gene, at least in part, contributes to the development of coroonary artery lesions in Kawasaki disease
- MMP-13 gene expression-related polymorphism is associated with risk for the highly aggressive form of oral cancer; the high expression A allele of the -77A/G polymorphism seems to be a prognostic factor for tumor progression
- MMP-13 is involved in disc histopathology, and that disc cells actively participate in the synthesis of extracellular matrix-degrading proteinases
- This review highlights MMP-13, expressed by chondrocytes and synovial cells in osteoarthritis and rheumatoid arthritis and thought to play a critical role in cartilage destruction.
- Chondrocytes were found to express IL-7 receptors and to respond to IL-7 stimulation with increased production of matrix metalloproteinase-13.
- potential role for MMP12 -82 A/G and MMP13 -77 A/G combined polymorphisms in superficial endometriosis. As no association was found with deep infiltrating endometriosis, these polymorphisms might protect from a more in-depth penetration of tissues.
- MMP8, but not MMP1 or MMP13, may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis
- MMP-13 may be particularly useful as a prognostic marker when evaluated along with Her-2/neu and lymph node status.
- CXCL12 enhances Laryngeal and hypopharyngeal squamous cell carcinoma cell invasiont through paracrine-activated CXCR4, triggering MMP-13 upregulation.
- increases in IL-6, MMP-13, and RANKL (receptor activator of nuclear factor kappa B ligand)expression in osteoarthritis (OA) subchondral bone osteocytes suggest that in subchondral bone OA progression involves abnormal osseous tissue remodeling
- SRC-3/AIB1 directly regulates transcription of matrix metalloproteinase (MMP)-2 and MMP-13 through its coactivation of AP-1 and PEA3.
- MMP-13 is over-expressed in renal cell carcinoma bone metastasis and is induced by TGF-beta1.
- The structural changes in the venous wall in addition to the increased expression of MMP-2, MMP-9 and MMP-13 in venous aneurysms suggests a possible causal role for these MMPs in their pathogenesis.
- Nuclear extracts from cells confirmed upregulation of active MMP-13 after oxygen and glucose deprivation
- Active MMP13 is suppressed in vivo and in vitro by estradiol and progesterone, suggesting a protective effect against vaginal supportive tissue deterioration.
- BMP-2 enhanced the invasiveness of chondrosarcoma cells by increasing MMP-13 expression through the PI3K, Akt, c-Fos/c-Jun and AP-1 signal transduction pathway.