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Validated All-in-One™ qPCR Primer for THBS1(NM_003246.3) Search again
Product ID:
HQP088549
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
THBS, THBS-1, TSP, TSP-1, TSP1
Gene Description:
thrombospondin 1
Target Gene Accession:
NM_003246.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq].
Gene References into function
- Increased expression is associated with breast tumor metastasis
- Immobilized TSP-1, as well as its 70-kDa proteolytic fragment containing the type 1 repeats, enhances IL-6 release from monocytic U937 cells stimulated with phorbol myristate acetate and LPS, whereas it inhibits IL-10 release.
- Stromally derived TSP-1 upregulates the production of MMP-9 and regulates matrix remodeling of tumor invasion in pancreatic adenocarcinoma
- T lymphocytes express thrombospondin-1 (TSP-1). Endogenous TSP-1 is part of an adhesion-dependent mechanism controlling cytoplasmic spreading and migration in T lymphocytes.
- Thrombin activation leads to the rapid and specific association of a large amount of secreted alpha-granular TSP-1 with the actin cytoskeleton.
- Interactions of thrombospondins with alpha4beta1 integrin and CD47 differentially modulate T cell behavior
- level spontaneously secreted by patient CLL B-cells quantified; consistently secreted in all patient samples.
- mediates adhesion of osteosarcoma to the core region of thrombospondin 1 (alpha 4 beta 1 integrin)
- structural characterization
- Anti-adhesive activity mediated by the N-terminal domain of cell surface calreticulin
- Interaction of the TSP1 module of complement component C8 beta subunit with the membrane attack/perforin domain determines the binding specificity of C8 beta toward C8 alpha.
- All adrenal tissues (control adrenals, nonfunctional adenomas and ACTH-dependent aldosterone-producing adenomas) expressed both TSP1 and TSP2 mRNAs. Correlated closely with the expression of ACTH receptor.
- a novel, antiparallel, three-stranded fold that consists of alternating stacked layers of tryptophan and arginine, capped by disulfide bonds; the front face contains a right-handed spiral, positively charged groove that might be the "recognition" face.
- thrombospondin-1 plays a role in the up-regulation of MMP-9 expression in gastric cancer.
- The anti-angiogenic type 1 repeat domains of TSP1 have been biophysically characterized and the disulfide bond connectivity of the peptides determined.
- Thrombospondin-1 is differently expressed in subcutaneous and visceral fat of obese subjects. The difference in expression between the depots was greater in women than in men.
- TSP1 is expressed in tumor stroma in colorectal cancer, inhibits tumor angiogenesis and suppresses tumor growth by activating TGF beta-1.
- Macrophage recognition and phagocytosis of apoptotic fibroblasts is critically dependent on this protein.
- Mutation of p53 gene endows gliomas with an angiogenic phenotype by reducing thrombospondin-1 production as well as enhancing the angiogenesis inducers in the early phase of malignant progression.
- the migration of retinal pigment cells in epiretinal membranes is modulated by TSP1 and SPARC and thus that these two proteins ultimately may represent therapeutic targets in the management of the membranes.
- TSP1 is the critical regulator of angiogenesis driven by ras proteins in a genetically defined tumor model.
- Ras modulates myc activity to repress TSP1 expression and increase tumor angiogenesis.
- TSP-1-induced inhibition of megakaryocytopoiesis is probably mediated in part by the binding of TSP-1 to CD36 expressed on the megakaryocytic progenitors
- interactions among the three structural motifs of the C-terminal region of this protein
- Our results suggest that transcriptional inactivation of TSP1 by aberrant DNA methylation of the promoter region may participate partly in stomach carcinogenesis through TSP1 down-regulation.
- thrombospondin-1 (4N1-1) stimulates platelet aggregation by a novel mechanism involving both an activation-independent agglutination and an activation-dependent, glycoprotein (GP) IIb/IIIa-mediated aggregation which involves GPVI signaling
- Thrombospondin-1 mediates platelet adhesion at high shear via glycoprotein Ib.
- Interaction of thrombospondin-1 with its ligand CD47 contributes to the perpetuation of inflammation in rheumatoid synovitis.
- thrombospondin-1 and thrombospondin-2 have N-terminal regions for binding to alpha6beta1 integrin
- TSP1 expression is significantly higher in malignant epithelial sources over normal and benign epithelial sources, but no difference in expression levels is evident between primary tumors with or without metastases.
- TSP-1 acts as a scavenger for matrix-associated angiogenic factors, affecting their location, bioavailability, and function.
- thrombospondin-1 has a role in motility and proteolytic activity of oral squamous cell carcinoma cells
- Hepatocyte growth factor mediates angiogenesis in cultured cancer cells by regulating the expression of VEGF and this protein.
- Expression of TSP-1 along with other genes suppresses neuronblastoma tumor growth.
- DC-derived TSP serves as a previously unappreciated negative regulator contributing to arrest of cytokine production, further supporting its fundamental role in vivo in the active resolution of inflammation and maintenance of steady state.
- ANG II-induced activation of latent TGF-beta1 in human mesangial cells operates via TSP-1.
- TSP-1 might mediate AGE-induced distal renal tubule hypertrophy. There are several putative transcription factor binding sites in the TSP-1 promoter, including those for AP-1, CREB, NF-kappaB, SRF, and HSF.
- results indicate that TSP-1 binding to low density lipoprotein-related protein does not require prior or concomitant interaction with cell surface heparan sulfate proteoglycans but suggest subsequent endocytosis requires high-affinity heparin-binding
- functional and structural differences between the Ser-700 and Asn-700 thrombospondin-1 variants
- crystal structure of a cell-binding TSP-1 fragment, spanning three T3 repeats and the C-terminal globular domain, reveals a compact assembly
- Review: thrombospondin-1 described as a modulator of angiogenesis through its role in regulating endothelial cell apoptosis, protease expression, and vascular endothelial growth factor expression
- This study showed for the first time that in human umbilical vein endothelial cells (HUVECs), 17beta-E2 transiently down-regulates the expression and secretion of a potent negative regulator of angiogenesis, thrombospondin-1 (TSP-1).
- downregulation of TSP-1 up to 18h following ultraviolet b light exposure
- No association of the TSP-1 polymorphism with myocardial infarction was found in an ethnic Chinese Han population in Beijing.
- Increased expression of TSP1 is associated with an aggressive angiogenic phenotype in melanoma.
- THBS 1 and VEGF are proangiogenic factors that stimulate angiogenesis in hepatocellular carcinoma
- beta(1) integrins mediate cell adhesion to the type 1 and type 2 repeats
- Data show that the WSXW motif binds the VLAL sequence on both latency-associated peptide and mature TGF-beta, and these interactions are critical for thrombospondin-1-mediated activation of the TGF-beta latent complex.
- results indicate that transforming growth factor TGFbeta1 treatment may regulate angiogenesis in pituitary cells by initially increasing levels of pro-angiogenic growth factor VEGF-A and then stimulating anti-angiogenic thrombospondin -1 and 2 levels
- TSP-1 from activated platelet releasate acts with vitamin D-binding protein to enhance complement 5a-mediated chemotaxis.
- Ser-700 polymorphism alters a rapidly filled, high affinity Ca2+ -binding site in the first Ca2+ -binding motif in thrombospondin-1 associated with familial premature coronary artery disease
- the circulating TSP-1/vascular endothelial growth factor balance is diminished in cancer patients
- Able to inhibit or stimulate tumor progression and angiogenesis, as well as affect wound healing, platelet aggregation, and arterial remodeling. Review.
- TSP-1 located at the border between tumor and stroma as observed in primary prostate tumors may act as a barrier of tumor growth
- Endogenous calreticulin binding to the N-terminal domain of endogenous thrombospondin-1 (TSP-1) elicits a motogenic signal to the T cells through the C-terminal domain of TSP-1 and its cell surface receptor integrin-associated protein (CD47).
- In human androgen-dependent localized prostate carcinomas, TSP1 expression was inversely correlated with blood vessel density.
- Thrombospondin 1, which plays a key role in initial immune defense, was found to be the most markedly up-regulated gene in blood after exposure to stents in vitro.
- thrombin formed at sites of vascular injury increases THBS1 expression into the extracellular matrix via activation of a PAR-1, G(i/o), G(q), EBOX/EGRF-signaling cascade
- TSP-1 may contribute to the regulation of neovascularization in the endometrium and gestational tissues.
- Thrombospondin-1 up-regulates expression of cell adhesion molecules and promotes monocyte binding to endothelium.
- Constitutive overexpression of TSP-1 may play an important role in autocrine TGF-beta signaling and accumulation of extracellular matrix in scleroderma fibroblasts.
- thrombospondin-1 is not necessary for proliferation but is permissive for vascular smooth muscle cell responses to platelet-derived growth factor
- TSP-1 could enhance conversion of latent TGF-beta1 into active TGF-beta1 in a cell-free system. Ox-LDL interacts with TSP-1 and suppresses subsequent TSP-1-dependent TGF-beta activation.
- an extracellular matrix glycoprotein implicated in the regulation of angiogenesis and tumour development
- Preincubation of erythroid cells with thrombospondin 1 eliminated the inhibitory activity of insulin-like growth factor binding protein 3
- Increased TSP1 levels in blood is asociated with non-small cell lung cancer
- Results suggest a physiological function of thrombospondin-1 binding to TSG-6 in regulation of hyaluronan metabolism at sites of inflammation.
- specific amino acids of the C-terminal cytoplasmic tail of CD36 are crucial for the in vitro angiostatic activity of TSP-1
- Transcripts for THBS1, an activator of TGF-Beta, increased during acute lung injury in mice
- positive- and negative-feedback loops exist between NO and TSP1; limiting TSP1 expression by positive feedback through the ERK mitogen-activated protein kinase pathway may facilitate switching to a proangiogenic state at low doses of NO
- Here we reported that the genomic lotus of TSP1 also transcribes a natural antisense transcript (NAT) with an overlapping and reverse complement region against TSP1.
- A387P in thrombospondin-4 and N700S in thrombospondin-1 polymorphisms perturb calcium binding sites
- results demonstrate a potent antagonism between TSP1 and proangiogenic signaling downstream of NO
- Thrombospondin 1 is a negatively prognostic factor for survival in resected colorectal liver metastases.
- Increased glomerular expression of thrombospondin-1 (THBS1), transforming growth factor beta 1 (TGFB1), and connective tissue growth factor (CTGF), occurs from the earliest stage of diabetic nephropathy.
- Data show that transcriptional control is not involved in the induction of thrombospondin-1 by heat shock.
- TSP-1 expression was associated with a significantly lower level of microvessel density.
- acute UV exposure downregulates TSP1 expression via PI3K-Akt activation in human keratinocytes
- Elevated glucose concentrations increased TSP-1 synthesis, which was associated with reduced cell proliferation, increased TGF-beta1 bioactivity, and stimulation of FN synthesis
- TSP-1 mRNA is a possible marker for contamination of thyroid-derived fibroblasts.
- IL-18 plays a key role in thrombospondin-1 expression involving JNK.
- Correlation of stromal TSP-1 expression with extracellular matrix components fibronectin and tenascin indicate its possible implication in tumour stroma remodeling.
- Edogenous TSP-1 connects separate cell-surface receptors functionally and regulates T-cell adhesion.
- apoptotic cell TSP-1, and notably its heparin-binding domain, creates a signalosome in iDCs to improve engulfment and to tolerate engulfed material prior to the interaction with apoptotic cells
- Anti-inflammatory extracellular matrix protein thrombospondin-1 promotes the generation of human peripheral regulatory T cells through the ligation of its receptor, CD47.
- These findings suggest that induction of apoptosis by camptothecin or doxorubicin in FTC-133 cells is greatly dependent by a down-regulation of TSP-1 expression and shed new light on a possible role for TSP-1 in drug resistance.
- cyclin D1 may have a central role in mediating invasion and metastasis of cancer cells by controlling Rho/ROCK signaling and matrix deposition of TSP-1 [review]
- By comparing the expression of p53, cyclin D1, p16, hTERT, and TSP-1 in spontaneously regressing keratoacanthoma and squamous cell carcinoma, the changes in the expression of these proteins to specific stages of skin carcinogenesis, is defined.
- thrombospondin-1 signaling has a role in tumor angiogenesis [review]
- In this review the authors provide mechanistic insight into the signaling pathways used by thrombospondin-1 and CD47 at the cellular and molecular level to prevent nitric oxide signaling through cyclic GMP, thereby exacerbating tissue ischemia.
- The prognosis was worst in patients with cancers that were VEGFA-positive and THBS1 methylation-positive.
- alpha9beta1 integrin expressed on microvascular endothelial cells interacts with thrombospondin-1, and this interaction is involved in modulation of angiogenesis.
- identified combined decrease of NFkappaB and increased TSP1 as molecular events underlying the AR anti-tumor activity in vivo
- Data show that thrombospondin-1 promotes cellular adherence of gram-positive pathogens via recognition of peptidoglycan.
- Upregulation of thrombospondin-1(TSP-1)in sporadic inclusion body myositis.
- Thrombospondin 1 induces a unique antiangiogenic response in certain types of vascular cells, blocks the cell cycle, and suppresses angiogenesis in vivo.
- two mAbs that recognize epitopes that are optimally present when three elements of signature domains of TSP-1 and TSP-2, the EGF-like modules, wire, and the lectin-like module, are in the same protein.
- EWS/FLI1 has a role in regulating tumor angiogenesis in Ewing's sarcoma via suppression of thrombospondins TSP1 and TSP2
- TSP-1 expression was detected in 17.5% of the cases with NHL, and is associated with aggressive histology and shorter overall survival.
- Patients with a truly negative sentinel node (pN0[i-][sn]) frequently have higher expression of TSP-1 that may have inhibited metastatic progression.
- These results identify syndecan-4 as a novel receptor for the N-terminus of TSP-1 and suggest that TSP-1 N-terminal pro-angiogenic activity is linked to its capacity of interfering with syndecan-4 functions in the course of cell adhesion.
- Study demonstrates that the fibroblast growth factor-2 binding site of thrombospondin-1 is located in the type III repeats.
- Sudy indicates that TRPC4 loss in renal cell carcinoma (RCC) leads to impaired Ca(2+) intake, misfolding, retrograde transport and diminished secretion of antiangiogenic TSP1, thus enabling angiogenic switch during RCC progression.
- TSP1 is an important link between adipocytes and macrophage-driven adipose tissue inflammation.
- Thrombospondin-1 was consistently underexpressed in both the edge and center of uterine fibroids.
- The cell-specific regulation of TSP-1 suggests a potential mechanism for the aberrant angiogenesis in diabetics and TSP-1 involvement in development of various vascular diabetic complications
- The meta-analysis included 6388 (TSP-1), 4930 (TSP-2), and 6978 (TSP-4) cases; none of the polymorphisms was found to be linked with the risk of myocardial infarction.
- TSP-1 A2210G variant has a dramatically lower prevalence in the Chinese Han population and fail to associate with myocardial infarction.
- The data document the importance of TSP-1 in promoting MMP-2-mediated cancer cell invasion.
- indicate connectivity between calcium binding sites in the C-terminal signature domain and the N-modules of thrombospondin-1 that regulates ligand binding and functional activities of the N-modules
- trichostatin A activates the transcription of TSP1 gene through the binding of transcription factor CBF to CCAAT box and the enhanced histone acetylation.
- Results suggest focal adhesion kinase is involved in thrombospondin-1-induced vascular smooth muscle cell migration.
- TSP-1 promotes human follicular thyroid carcinoma cell invasion mainly through up-regulation of the urokinase-dependent activity.
- the epigenetic inactivation of TSP-1 and RASSF1-A genes is associated with pathological variables and seems to be of prognostic significance in penile cancer.
- These results suggest that the hypoxia induces the migration of coronary artery smooth muscle cells, and that the migration is elicited by TSP-1 of which induction is fully dependent on the stabilization of HIF-1alpha, in autocrine regulation.
- a novel role of TSP-1 to inhibit tumor-stroma reaction that could be attributed to the blockage of activated fibroblasts from invading cancer cells.
- The aberrant methylation of TSP1 is a novel epigenetic mechanism for suppressing TGF-beta signaling in colorectal cancer.
- although Thrombospondins harboring the Ser allele binds a full complement of calcium ions, repeat 1C is altered, leading to destabilization of surrounding structures
- The constitutively active form of AhR induced activation of the thrombospondin-1 gene promoter.
- The effects of TSP-1 on endothelial cell migration are mediated by TGF-beta1, whereas the effects on fibroblast and tumour cell migration are TGF-beta1-independent.
- HuR plays an important role in translation of the TSP1 mRNA.
- a novel role for TSP1 signaling through the calreticulin/LRP1 co-complex in tissue remodeling and fibrotic responses through stimulation of anoikis resistance.
- recent results dealing with regulation of TSP-1 expression by epidermal growth factor & hepatocyte growth factor; results show TSP-1 can have opposite effects on cell invasion depending upon type of differentiated thyroid carcinoma studied [review]
- This study demonstrates increased plasma levels of TSP1, which correlated with increased levels of proinflammatory cytokines in plasma of rheumatoid arthritis patients.
- TSP-1's C-terminal domain opens to interact with the CD-47 receptor
- The positive correlation of glomerular p-smad2/3 positivity with proteinuria further supports the importance of the TSP-1/TGF-beta system as a relevant mechanism for progression of type-2 diabetic nephropathy
- Decreased TSP-I expression may explain reduced synaptogenesis and altered hippocampal ultrastucture, both of which may contribute to the pathogenesis of diabetic dementia.
- This observation highlights the role of TSP-1 in hemorrhagic fever with renal syndrome which might have further prognostic and therapeutic implications.
- TSP1 may play an important role in antitumor immunity by enhancing recruitment and activation of M1 tumor associated macrophages, which provides an additional selective pressure for loss of TSP1 and TSP2 expression during tumor progression
- a profound increase in TSP-1 levels and a corresponding increase in PAI-1 activity by insulin in vivo