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Validated All-in-One™ qPCR Primer for GATA6(NM_005257.5) Search again
Product ID:
HQP088468
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
-
Gene Description:
GATA binding protein 6
Target Gene Accession:
NM_005257.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- GATA-4 and GATA-6 mRNAs are readily detectable from gestational week 19 in human adrenal cortex.
- role in trans-activation of bone morphogenetic protein 4 and regulation of mammalian organogenesis
- The activation of PPARgamma-dependent pathways induces the differentiated phenotype in proliferative VSMCs, and this induction is mediated, in part, through a GATA-6-dependent transcriptional mechanism.
- GATA-6 did not correlate with apoptosis or cell proliferation and is therefore unlikely to be directly involved in these processes in the human fetal testis
- GATA-6 was expressed in most yolk sac tumors examined and also in nonmalignant tissues including gut/respiratory epithelium, sebocytes, and neuroepithelium in mature and immature teratomas
- Normal postnatal morphogenesis of the lung depends upon precise temporal-spatial regulation of GATA-6.
- Adrenal uses GATA-6 to enhance transcription of steroid-metabolizing enzymes needed to produce dehydroepiandrosterone sulfate. Additionally, GATA-6 works in synergy with SF1 to maximally increase expression of enzymes needed to produce adrenal androgens.
- Both steroidogenic factor 1 and GATA-6 were positive regulators of Steroid sulfotransferase 2A1 promoter constructs
- Steroidogenically active human adrenocortical cells were weakly positive for GATA-4, whereas steroidogenically inactive cells were totally GATA-4 negative. In contrast, both cell lines expressed GATA-6.
- cytochrome b5 gene transcription is regulated by Sp3, GATA-6, and steroidogenic factor 1 in human adrenal NCI-H295A cells
- analysis of cAMP-dependent proteolysis of GATA-6
- GATA-6 detected in ovarian GCTs has retained normal function; exclusive expression of GATA-6 and HNF-4 in endodermal tissues indicates these transcription factors play role in differentiation of germ cells towards endodermal phenotype
- expression of GATA-4 and GATA-6 is up-regulated prior to the transcriptional activation of Nkx 2.5 during cardiogenesis
- GATA6 is expressed in adult mouse brain and several regions of adult human brain. GATA6 is found in neurons, astrocytes, choroids plexus epithelium, and endothelial cells.
- In polycystic ovary syndrome(PCOS) theca cells, GATA6 gene transcription and stability of GATA6 mRNA are increased. Increased gene transcription and mRNA stability are likely due to intrinsic differences in PCOS theca cells.
- Altered histone modification of the promoter loci is one mechanism responsible for the silencing of GATA transcription factors.
- Results identify a conserved function for endodermal GATA transcription factors GATA6 and ELT-2 in regulating local epithelial innate immune responses.
- WNT8B expression in hepatocyte progenitors derived from human ES cells is due to POU5F1 (OCT3/OCT4) and GATA3, and WNT8B expression in diffuse-type gastric cancer is due to POU5F1 and GATA6
- Cooperative interaction between HNFA4 and GATA4 and GATA6 regulates ABCG5 and ABCG8.
- functional role for GATA-6 in regulation of bladder smooth muscle differentiation
- GATA-6 expression is involved in the regulation of corticosteroid production in both the human fetal and adult adrenals, as well as in adrenocortex development.
- Results describe the cyclic AMP-dependent proteolysis of GATA-6 expressed on the intracellular membrane.
- We demonstrated that overexpression of GATA-6 in fibroblasts inhibited basal levels, as well as markedly decreased IL-4- and TGF-beta-induced TN-C mRNA and protein levels.
- the majority of the ovarian surface epithelial carcinomas retained GATA-4 expression, whereas approximately two-thirds of the carcinomas had mislocalization or loss of GATA-6 expression
- The results suggest differential but overlapping functions for GATA-4 and GATA-6 in the normal gastrointestinal mucosa. Furthermore, GATA-4, GATA-6 and Ihh expression is altered in premalignant dysplastic lesions and reduced in overt cancer.
- MEK-ERK-dependent phosphorylation of GATA-6 plays a role in upregulation of transcription of Nox1
- GATA6 amplification and overexpression contribute to the oncogenic phenotypes of pancreatic cancer cells.