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Validated All-in-One™ qPCR Primer for ABCC2(NM_000392.4) Search again
Product ID:
HQP067276
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ABC30, CMOAT, DJS, MRP2, cMRP
Gene Description:
ATP binding cassette subfamily C member 2
Target Gene Accession:
NM_000392.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport.
Gene References into function
- Two novel mutations in the multidrug resistance protein 2 gene in Israeli patients with Dubin-Johnson syndrome.
- No change in cMOAT mRNA expression was detected in non-HTLV-1 Tax producing ATL cell lines following transfection with HTLV-1 Tax.
- structural requirements for apical sorting
- Impaired protein maturation followed by proteasomal degradation of inactive MRP2I1173F explain the deficient hepatobiliary elimination observed in this group of Dubin-Johnson syndrome patients.
- mutation in MRP2 causes deficient maturation and impaired sorting in cells in Dubin-Johnson syndrome
- RNA expression of this protein in breast cancer correlates with response to chemotherapy.
- The role of multidrug resistance proteins MRP1, MRP2 and MRP3 in cellular folate homeostasis.
- study of the interactions of drugs, organic anions, and bile acids with MRP2 substrates shows MRP2 contains two similar but nonidentical ligand binding sites: a substrate transport site and a site that regulates the affinity of the transport site
- MRP2 active transport of estradiol-17-beta-d-glucuronide and MRP2 ATPase characteristics in isolated, inside-out membrane vesicles
- MRP1 and MRP2 were expressed in peripheral blood cells, with more than sevenfold higher MRP1 expression. The MRP2 mRNA expression was highest in CD4+ cells, followed by CD8+ > CD56+ > CD19+ cells.
- Homozygous mutation Arg768Trp in the ABC-transporter encoding gene multidrug-resistance-associated-protein 2 causes Dubin-Johnson syndrome
- Disrupted localization of radixin and MRP2 supports the concept that radixin contributes to the canalicular localization of MRP2.
- Cholestasis promotes down-regulation of MRP2 expression in the duodenum of humans.
- ABCC2 promoter polymorphism is not a determinant of the risk of spinal dysraphism.
- "MRP2(multidrug resistance-associated protein 2) is associated with active drug efflux and may influence oral bioavailability of common classes of drugs.
- Dysfunction or loss of the multidrug resistance protein 2 (MRP2) is the molecular basis of Dubin-Johnson syndrome (DJS).
- The major canalicular transporter genes are expressed at mid-gestational stage during human fetal development.
- ABCB1, ABCC1, ABCC2, and ABCG2 haplotypes influence response to nelfinavir
- nuclear factor, erythroid derived 2, like 2 appears to regulate Mrp2 gene expression via an antioxidant-response element element located at the proximal region of its promoter in response to exposure to xenobiotics
- enhanced expression of MRP2 and lower expression of AQP3 are responsible for lower arsenic accumulation in arsenic-resistant cells
- ABCC2 genotype modulates the expression in the unaffected renal cortex of renal cell cancer patients
- The mRNA induction of MDR1, MRP1, MRP2 and MRP3 by rifampicin (Rif), dexamethasone (Dex) and omeprazole (Ome) was investigated in primary cultures of cryopreserved human and rat hepatocytes.
- The ABCC2 c.1446C>G SNP is associated with reduced systemic exposure to pravastatin as a consequence of increased MRP2 expression.
- the C-24T SNP of MRP2 is associated with a lower oral clearance of mycophenolic acid in steady-state conditions
- ABCC2 polymorphism contributes to variability of methotrexate kinetics.
- The expression of ABCC2 in nuclear membranes in human tissues is specific for poorly differentiated cells including stem cells.
- Human corneal epithelium expresses the multidrug resistance associated protein ABCC2. ABCC2 contributes to drug efflux from the eye.
- Allelic variants of UGT2B7, CYP2C8, and ABCC2, which may predispose to the formation and accumulation of reactive diclofenac metabolites are associated with diclofenac hepatotoxicity
- I1173F causing DJS in Iranian Jews occurred after the separation of Iranian Jews from Moroccan Jews 2000-2600 years ago, while A244V causing FVII deficiency in Iranian and Moroccan Jews occurred prior to the divergence of these two populations.
- Rotor-type hyperbilirubinaemia is not an allelic variant of ABCC2 deficiency.
- Results show that the molecular model of ABCC2 agreed well with experimentally determined ABCC2-ligand interactions and the interaction of ABCC2 with quercetin glucuronides is dependent on the position and nature of substitution.
- Mrp2 and Mrp3 provide alternative routes for the excretion of a glucuronidated substrate from the liver in vivo.
- MRP1-Pro(1150), MRP2-Pro(1158), and MRP3-Pro(1147) in the cytoplasmic loop 7 differ in their influence on substrate specificity but share a common role in the nucleotide interactions at nucleotide binding domain 2 of these transporters.
- 12 polymorphisms and mutations were found in the MRP2 gene in a Korean population.
- Inhibition of renal uptake (via OAT3) and efflux processes (via MRP2 and MRP4) explains the possible sites of drug-drug interaction for methotrexate with probenecid and some NSAIDs, including their glucuronides.
- neither MRP1 nor MRP2 appears to have a role in response to cisplatin-based chemotherapy in resected non-small cell lung cancer
- Report disturbed colocalization of multidrug resistance protein 2 and radixin in human cholestatic liver diseases.
- Radixin and ezrin play similar roles in the apical membrane localization of ABCC2 (MRP2) and their expression level and subcellular distribution are important factors in the regulation of ABCC2 (MRP2) at the post-transcriptional level.
- This study showed that manipulation of drug efflux transporters may be a useful strategy for increasing the intracellular concentration and thereby enhancing the clinical efficacy of lopinavir.
- we observed that haplotype frequency of the ABCC2 gene in intrahepatic cholestasis of pregnancy patients significantly differed from controls
- induction of ABCC2 and ABCG2 by tBHQ is mediated by the Nrf2/Keap1 system, whereas the induction of ABCC1 may involve a Keap1-dependent but Nrf2-independent mechanism.
- There are important species differences in the transport efficiency of MRP2 substrates and in the modulation of transport by other compounds that should be taken into account, when results obtained in mice are extrapolated to humans.
- SNPs in ABCC2 and may predict the risk of leukopenia/neutropenia induced by docetaxel chemotherapy.
- ABCC2 1249G>A (V417I) variant was associated with lower oral bioavailability and increased residual clearance of intravenous talinolol
- The regions required for apical membrane localization of MRP2 are identified.
- No association between the ABCC2 genotypes or haplotypes, and the responsiveness of anti-epileptic drugs in Japanese epileptic patients was observed.
- Expression and localization MRP2 were significantly higher in hepatoid than in control adenocarcinoma
- The effects of single nucleotide polymorphisms (SNPs) of the uridine glucuronosyltransferase 1A9 (UGT1A9) and MRP2 genes (ie, coding for the UGT1A9 and the multidrug resistance protein transporter MRP2)-both involved in MPA metabolism.
- Celecoxib upregulates MRP2 in colon cancer and results in lack of synergy with standard chemotherapy.
- Dubin-Lohnson syndrome is due to the absence of an cMOAT from the apical membrane of hepatocytes, because of mutation in the MRP2 gene - ABCC2.
- the expression of MRP(multiple drug resistance-associated protein)1, MRP2, and MRP3 molecules in systemic lupus erythematosus mononuclear cells was not different from normal
- ABCC2 polymorphism on the pharmacokinetics of mycophenolic acid (MPA) and its metabolites phenolic glucuronide (MPAG) and acyl glucuronide (AcMPAG) in Chinese renal transplant recipients.
- Three novel nonsynonymous variations: 2686G > A, 4240C > T and 4568A > C were detected in single heterozygous Malay, Chinese, & Indian subjects. Among the novel nonsynonymous variations, 4240C > T & 4568A > C were predicted to be functionally significant
- ABCC2 genotype is one of the predictors of the variability of irinotecan pharmacokinetics in Japanese patients with metastatic colorectal cancer receiving FOLFIRI.
- Interaction of hepatocyte nuclear factors in transcriptional regulation of tissue specific hormonal expression of human multidrug resistance-associated protein 2 (abcc2).
- Multidrug resistance-associated transporter 2 regulates mucosal inflammation by facilitating the synthesis of hepoxilin A3.
- Expression of multidrug resistance-associated protein 2 is involved in chemotherapy resistance in human pancreatic cancer.
- The effect of cholesterol depletion on the transport function of MRP2, and whether detergent-resistant bile canalicular membrane domains association is necessary for MRP2 transport function were studied.
- Posttranslational regulation of Abcc2 expression by SUMOylation system.
- Single nucleotide polymorphism analysis of the ABCC2 protein may become an index of the treatment for continuation