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Validated All-in-One™ qPCR Primer for TAGLN(NM_003186.4) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Gene References into function
- loss of transgelin gene expression may be an important early event in tumor progression and a diagnostic marker for breast and colon cancer development.
- Transgelin functions as a suppressor to inhibit prostate cancer cell growth
- crystallization and X-ray diffraction of transgelin.
- SM22 expression in SMCs was dramatically higher than in GC cells, which indicates that SM22 is unlikely to be a proper biomarker for GC.
- tagln is a novel target of TGF-beta/Smad3-dependent gene expression in alveolar epithelial type II cells
- 2.3 A resolution crystal structure of full length human transgelin, whose main structural feature is confirmed to be a CH domain.
- Identificaton of transgelin a novel biomarker for gastric adenocarcinoma.
- Selective overexpression of airway smooth muscle genes in asthmatic airways leads to increased Vmax, thus contributing to the airway hyperresponsiveness observed in asthma.