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Validated All-in-One™ qPCR Primer for MS4A1(NM_152866.2) Search again
Product ID:
HQP065603
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B1, Bp35, CD20, CVID5, FMC7, LEU-16, S7
Gene Description:
membrane spanning 4-domains A1
Target Gene Accession:
NM_152866.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members.
Gene References into function
- review of structure, function, tissue and cell distribution
- CD20 functionally associates with the B cell receptor (BCR) on the surface of stimulated B lymphocytes, then rapidly dissociates before BCR internalization.
- Differences in complement recruitment correlated with the redistribution of CD20 in the cell membrane following mAb ligation
- presence of this antigen, but not CD3 antigen in T-cell prolymphocytic leukemia
- FMC7 antigen is identified as a CD20 epitope; sensitivity to cholesterol reveals conformational state of CD20 that is regulated by cholesterol
- raft-associated CD20 constitutes a component of a SOC entry pathway activated by the B cell receptor
- CD20 calcium channel is localized to microvilli and constitutively associated with membrane rafts
- a marker of follicular B-cell non-Hodgkin's lymphoma
- Estimating the expression of CD20+ lymphocytes in the antrum mucosa in children, infected with Helicobacter pylori and after bacterial eradication.
- Neither upregulation of CD20 antigen nor a change of the proportion of CD20 positive cells was observed after a dose of 5 microg/kg GM-CSFin B cell chronic leukemia.
- CD20 may have a role in steroid-resistant renal allograft rejection and reduced graft survival
- expression in bacterial cells; isolation and biophysical and structural studies
- PegIFN does not appear to upregulate CD20 expression in peripheral lymph node tumor cells
- analysis of CD5-positive diffuse large B-cell lymphoma with the unusual phenotype of cytoplasmic CD20 (+), surface CD20 (-) [case report]
- CD20, a trans-membrane B-cell-specific antigen, is a potential target for treatment of certain malignant and nonmalignant plasma cell disorders including refractory idiopathic thrombocytopenic purpura.
- Rituximab may be effective in patients with multiple myeloma expressing this antigen.
- human tonsil contains long-lived plasma cells, the majority of which express CD20
- binding of Gb(3)/CD77 by its cognate ligand transmits information within the lipid bilayer of model lymphoma cells to impact the behaviour of selective proteins, most notably CD20, via a mechanism influenced by the level of cholesterol within the membran
- Possibly detected in B lymphocytes in renal allograft biopsy specimens and might be useful as a prognostic marker.
- Monitoring of serum HCV RNA levels and transaminases may be helpful to understand the cause of liver dysfunction in patients receiving chemotherapy
- CD20-targeted and convertase-armed measles virus can synergize with fludarabine
- current study showed that the level of CD20 was differentially expressed among fluorescent in-situ hybridization subtypes of chronic lymphocytic leukaemia (CLL); patients with trisomy 12 CLL showed strong leukemic cell CD20 expression
- Chimeric 2H7 (C2H7) can mediate complement dependent cytotoxicity and antibody-dependent cellular cytotoxicity effects on CD20 positive human Burkitt lymphoma cells.
- CD20 induces cytosolic calcium flux through its ability to associate with and "hijack" the signaling potential of the BCR
- Statistically significant difference in disease free survival has been found between groups with and without CD20 expression in patients with Hodgkins lymphoma
- CD20 expression is frequent in classical Hodgkin's Lymphoma and our results are in consensus with reported literature on this subject.
- Results identify a subpopulation of CD133(+) cells expressing the B-cell marker CD20, which display myogenic properties.