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Validated All-in-One™ qPCR Primer for ATG5(NM_001286106.1) Search again
Product ID:
HQP065365
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5
Gene Description:
autophagy related 5
Target Gene Accession:
NM_001286106.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p
- the Apg12p.Apg5p conjugate does not facilitate LC3 processing
- Apg5 and the apoptosis-specific protein p45ASP are distinct proteins although they do share some common characteristics.
- results suggest that autophagy 5-like Atg5 plays a crucial role in interferon-gamma-induced autophagic cell death by interacting with Fas-associated protein with death domain (FADD)
- Our data show that ATG5 protein is required to execute Denge Virus 2-induced autophagy.
- The UBQLN protective effect requires the autophagy-related genes ATG5 and ATG7, two essential components of autophagy.