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Validated All-in-One™ qPCR Primer for CX3CR1(NM_001171174.1) Search again
Product ID:
HQP064965
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28
Gene Description:
C-X3-C motif chemokine receptor 1
Target Gene Accession:
NM_001171174.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- CX3CR1 tyrosine sulfation enhances fractalkine-induced cell adhesion
- Fractalkine receptor CX3CR1 is selectively expressed on various lineages of lymphocytes with high contents of intracellular perforin and granzyme B.
- CX3CR1 was up-regulated during chronic injury in areas of portal and lobular inflammation.
- CX3CR1 has a role in the accumulation of intrarenal inflammatory cells in kidney diseases
- CX3CR1 gene is controlled by three distinct promoter regions, which are regulated by their respective untranslated exons and that lead to the transcription of three mature messengers
- a difference in genotype frequencies of the V249I and T280M polymorphisms in CX(3)CR1 between peripheral arterial disease patients and controls could not be detected
- a subset of mononuclear cells expresses high levels of fractalkine in human coronary atherosclerotic plaques and smooth muscle cells within the neointima express the fractalkine receptor CX3CR1
- Two novel CX3CR1 isforms are described that have increased sensitivity to their CX3CL1 and HIV gp120 ligands in binding and functional assays
- upregulated in circulating CD4(+) T lymphocytes in symptomatic allergic rhinitis and asthmatic patients
- existence of CX(3)CR1 (fractalkine receptor) in ejaculated sperm and present findings suggest that fractalkine in the Fallopian tube contributes to immunodefence mechanism during fertilization and to sperm motion in the oviduct
- the naturally occurring Ile249-Met280 variant of the chemokine receptor CX3CR1 has enhanced adhesive capacities
- Polymorphisms of CX3CR1 are a genetic risk factor for internal carotid artery occlusive disease.
- fractalkine (FKN) and CX(3)CR1 expression in the skin, serum sFKN levels, and CX(3)CR1 expression on blood leukocytes in patients with atopic dermatitis
- A decrease, caused by sequence variation (V249I, T280M) and/or lower CX3CR1 expression, in CX3CR1-induced cellular activities could contribute to age-related macular degeneration development.
- CX3CR1-fractalkine expression regulates cellular mechanisms involved in adhesion, migration, and survival of human prostate cancer cells.
- Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms
- Replacement of the fractalkine N terminus with the cognate domain of vMIP-II disrupted the ability of FKN to bind CX3CR1.
- distribution assessed in endometrium across the menstrual cycle, in early pregnancy & in women using progestin-only contraceptives; colocalized with CX3CL1 to glandular epithelium and decidualized stromal cells, with highest expression in secretory phase
- We propose that the extra adhesion of monocytes observed in individuals carrying rare alleles of CX3CR1 may favor mechanisms leading to stroke.
- Fractalkine and CX3CR1 are involved in the recruitment of intraepithelial lymphocytes of intrahepatic bile ducts.
- V249I polymorphism is not associated wiwth hepatocellular carcinoma.
- The impact of CX3CR1 polymorphisms on HIV-1 pathogenesis and infection progression in children is reported.
- CX3CR1 receptor polymorphisms V249I and T280M have opposite effects in the development of acute coronary syndrome
- CX3CR1 is downregulated in patients with multiple sclerosis
- CX3CL1/CX3CR1 dyad may contribute to atherogenesis and plaque destabilization in human coronary artery disease
- CX3CR1 249I variant allele is more frequent in Spanish HIV-1-infected long-term nonprogressors of more than 15 years
- In heart transplantation, outcomes of early and late rejection episodes may be influenced by genetic variant interactions such as "CX3CR1 249I*CCR5 No-E" and "CCR5 E*RANTES -403A."
- CX3CR1 is critically dependent on the two negatively charged residues Asp25 and Glu254 located on the N-terminal domain and third extracellular loop, respectively.
- Genetic variation of the fractalkine CXC3R1 system could be involved in systemic sclerosis-associated pulmonary arterial hypertension.
- Presence of M280 polymorphism of fractalkine receptor CX3CR1 is associated with decreased common carotid artery intima-media thickness. Presence of I249 polymorphism does not play major role on progression of carotid atherosclerosis.
- Polymorphisms related to a functional decrease in ligand binding activity of CX3CR1 are associated with disease in patients with retinal vasculitis.
- Results may explain at the molecular and cell biology levels the genetic link between CX3CR1 and atherosclerosis.
- CX3CR1 may play an important role in the development of interstitial fibrosis via mononuclear cell-induced cytokine production chronic kidney graft rejection.
- role for the CX3CL1-CX3CR1 system in the pathogenesis of psoriasis
- Five CX3CR1 single nucleotide polymorphisms have significant associations between their common alleles and asthma.
- increased production of fractalkine by mucosal microvascular cells and increased numbers of circulating and mucosal CX3CR1+ cells in IBD point to a significant role of FKN in disease pathogenesis
- CX3CR1 and CX3CL1 mediate heterotypic anchorage of foam cells to coronary artery smooth muscle cells in the context of atherosclerosis
- Interactions with FKN via chemotactic and adhesive processes might contribute to accumulation of CD16+ monocytes expressing CX3CR1 at synovial tissue of rheumatoid arthritis and continuation of inflammation at site.
- Amino acid changes in CX3CR1 may influence the development of atopy but not asthma in German children.
- renal fibrosis is associated with the expression of CX(3)C receptors on human renal fibroblasts; 0expression is induced by reactive oxygen species
- A study evaluating 2 polymorphisms of CX3CR1 in colorectal cancer (CRC) in CRC to find out whether they occur more often in patients with CRC than in non-CRC individuals.
- results revealed improved immunologic response to HAART in HIV-1 infected patients with the CX(3)CR1-249I or CX(3)CR1-280M allele.
- CX3CL1/CX3CR1 axis might play a role in the induction and development of the endothelial dysfunction during rheumatoid arthritis
- we could not observe any association between CX3CR1 polymorphism and the risk of HTLV-1 associated myelopathy
- s-FKN is shed from endothelial cells following hypoxia/reoxygenation and acts through CX3CR1 on them to increase ICAM-1 expression and promote neutrophil adhesion through activation of the Jak-Stat5 pathway
- fractalkine-CX(3)CR1 interaction resulting in recruitment of natural killer (NK) cells and monocyte-macrophages plays an important role in mediating endothelial damage in cytomegalovirus infection
- Upregulation of CCL18 expression and downregulation of CX3CR1 expression play a role in immune responses against the adult t-cell leukemia cells.
- Fractalkine-induced activation of CX3CR1 by endothelial cells leads to in vivo angiogenesis.
- CX3CL1 may be involved in dissemination of lymphoma.
- The fractalkine receptor CX3CR1 is involved in liver fibrosis due to chronic hepatitis C infection.
- CX3CL1+ and CX3CR1+ cells are present in abdominal aortic aneurysms and may contribute to the recruitment of inflammatory cells.
- Decreased expression of the fractalkine receptor CX3CR1 on circulating monocytes as new feature of sepsis-induced immunosuppression.
- VLA-4, CX3CR1, and CXCR4, involved in T-cell homing, had increased expression in ITP patients
- I249 mutation was associated with multiple coronary vessel disease in older patients, while V249 homozygosity was associated with the early-onset coronary artery disease.
- In Crohn's disease, V249I CX3CR1 polymorphism is associated with intestinal strictures, particularly in smokers. This association is independent of CARD15 mutations.
- One function of the CX3CR1-CX3CL1 pathway is to recruit and sustain divergent immune cell populations implicated in the pathogenesis of cigarette smoke-induced emphysema.
- Increased CX3CL1/CX3CR1 interaction could contribute to the inflammatory phenotype seen in subgroups of CVID patients.
- study shows that the CX3CR1 receptor may be involved in pancreatic ductal adenocarcinoma tumor neurotropism and is a relevant and independent risk factor to predict an early local tumor relapse in resected patients
- The common CX3CR1 allelic variant was associated with increased GBM survival and with reduced tumor infiltration by microglia. The CX3CR1 polymorphism does not seem to be a risk factor for GBM but may prove useful in predicting survival.
- Prostate cancer cell chemotaxis seems, at least partially, dependent on membrane-bound CX3CL1/fractalkine ligand for chemokine receptor CX3CR1.