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Validated All-in-One™ qPCR Primer for C4B(NM_001002029.3) Search again
Product ID:
HQP057444
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
C4B1, C4B12, C4B2, C4B3, C4B5, C4BD, C4B_2, C4F, CH, CO4, CPAMD3
Gene Description:
complement C4B (Chido blood group)
Target Gene Accession:
NM_001002029.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Allelic distribution of complement components BF, C4A, C4B, and C3 in Psoriasis vulgaris.
- C4A and C4B gene-dosage variations play in infectious and autoimmune diseases.
- Genetic sophistication of human complement components C4A and C4B and RP-C4-CYP21-TNX (RCCX) modules in the major histocompatibility complex.
- C4b and C3b do not undergo the same conformational changes upon binding to the C4BP mutants as during the interaction with the wild type C4BP, which then results in an observed loss of the cofactor activity
- complex of C4b and protein S could act as a bridge between coagulation and inflammation due to the involvement of C4BP in regulating complement activation.
- negative effect of C4B(*)Q0 on health or survival
- C4b-binding protein-protein S complex inhibits the phagocytosis of apoptotic cells
- Plasma-derived PROS-C4BP complex has direct anticoagulant activity; enhanced direct activity of PROS-Heerlen-C4BP may compensate for low free protein S levels and low cofactor activity in individuals with protein S-Heerlen.
- Acute rejection of kidney transpl with C4d expression was diffuse and showed a higher proportionate elevation of serum creatinine at biopsy and 4 weeks after diagnosis.
- C4 null alleles were significantly more common in Henoch-Schonlein purpura patients than in controls
- multicenter analysis of C4d staining in protocol biopsies from renal allografts
- The tertiary structures of C4A and C4B were compared using near and far-UV circular dichroism, ANS fluorescence, site-specific monoclonal antibodies and isoelectric focusing.
- C4d is a possibe marker for the identification of humoral rejection in any clinical setting after kidney transplantation.
- C4d peritubular capillary expression did not differentiate patients after kidney transplantation immunosuppression , but it predisposes to progression of chronic morphological findings during 1-year observation.
- C3d was somewhat more predictive of margination than C4d in ABO-incompatible renal allografts
- Results describe three distinct profiles of serum complement C4 proteins in pediatric systemic lupus erythematosus (SLE) patients, and show tight associations of complement C4 and C3 protein levels in SLE but not in healthy subjects.
- 47% of the C4 genes adjacent to the RP2 gene were the short gene and 53% were the long gene
- C4d could be used as a marker for rejection following hepatic transplantation.
- Both galactose-specific and mannose-specific mannose-binding lectins isolated from common carp were found to associate with a serine protease that cleaves native human C4 into C4b but not C4i
- suggest a direct role of lgtC expression in the inhibition of C4b deposition and consequent serum resistance of R2866
- C4 gene deficiencies are associated with predisposition to chronic periodontitis.
- demonstrated a significant association between diffuse C4d staining, production of donor-specific antibodies, and graft failure
- findings indicate that the C4B*Q0 genotype can be considered as a major covariate of smoking in precipitating the risk for acute myocardial infarction and associated deaths
- Gene dosage variation and associated polymorphisms of C4B in systemic lupus erythematosus were studied.
- C4B*Q0 was present in two out of the 130 SLE patients; overall, our results do not demonstrate a significant association to these known C4 mutations in the Malaysian scenario
- the binding of Factor H and C4bp to Aspergillus spp. appears to be even stronger than to Candida spp. and different, albeit possibly nearby, binding moieties mediate this surface attachment.
- partial or complete C4b deficiencies were found in oligoarthritis and polyarthritis patients
- The binding of complement c4b and complement C3b to the proteins of Neisseria gonorrhoeae and N. meningitidis is reported.
- This observation indicates that low C4B copy number is a strong risk factor for short-term mortality after acute myocardial infarction (AMI) in smoking Icelandic patients.
- C4d immunostaining for the diagnosis of acute antibody-mediated rejection in renal transplant recipients
- Establish reproducible procedure for C4d detection with a polyclonal antibody.
- In 2,250 genetic typings of autistic subjects, only one individual carried a chromosome containing both C4B null allele and CYP21A2 mutations.
- Confirmed the independent prognostic value of peritubular capillary C4d staining on renal allograft survival in Chinese.
- omplement C4d region staining in peritubular capillaries in kidney allograft biopsies is a hallmark of antibody-mediated rejection.
- The finding of diffuse C4d on follow-up biopsy is significantly associated with kidney graft loss at 1 year, regardless of index biopsy C4d results. [C4d, complement 4d]
- Complement 4d in renal transplant biopsy is indicative of chronic graft rejection.
- HLA-specific antibodies are associated with vascular C4d deposition and soluble C4d in broncho-alveolar lavage of lung allografts
- C4BP binds to jeopardized cardiomyocytes early after acute myocardial infarct and co-localizes to other well known markers such as complemenb 3b.
- platelet C4d is associated with severe acute ischemic stroke; platelet C4d may be a biomarker as well as pathogenic clue that links cerebrovascular inflammation and thrombosis
- Measurement of the E-C4d/erythrocyte complement receptor 1 ratio may be a noninvasive method for detecting acute rejection after cardiac transplantation.