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Validated All-in-One™ qPCR Primer for GATA2(NM_001145661.1) Search again
Product ID:
HQP057186
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
DCML, IMD21, MONOMAC, NFE1B
Gene Description:
GATA binding protein 2
Target Gene Accession:
NM_001145661.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages.
Gene References into function
- The GATA-2 transcription factor interacts with the human eNOS promoter at a region which includes a putative GATA site [-230 to -226].
- Interactions of GATA-2 with the promyelocytic leukemia zinc finger (PLZF) protein, its homologue FAZF, and the t(11;17)-generated PLZF-retinoic acid receptor alpha oncoprotein.
- Essential and instructive roles of GATA factors in eosinophil development.
- regulates hematopoiesis
- GATA-1 and GATA-2 gene expression is related to the severity of dysplasia in myelodysplastic syndrome.
- REVIEW: Roles of hematopoietic transcription factors GATA-1 and GATA-2 in the development of red blood cell lineage
- Suppression of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy.
- GATA-2 exists as an acetylated protein in immature precursor cells; GATA-2 was acetylated in vitro by p300 and GCN5 at multiple acetylation sites, increasing its DNA-binding activity.
- there is functional cross talk between RA and GATA-2-dependent pathways
- Erythropoietin (Epo) gene expression is under the control of hypoxia-inducible factor 1 (HIF-1), and is negatively regulated by GATA2.
- Results show that in addition to its previously recognized function in suppressing PPARgamma transcriptional activity, interaction of GATA-2 and -3 with C/EBP is necessary for their ability to negatively regulate adipogenesis.
- insulin induces GATA2 phosphorylation on serine 401 in a PI-3K/Akt-dependent manner, impairing GATA2 translocation to the nucleus and its DNA binding activity
- GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene.
- Gata2 can operate independently of neuronal differentiation
- identification of 5 single nucleotide polymorphisms significantly associated with early-onset coronary artery disease; observations identify GATA2 as a novel susceptibility gene for coronary artery disease
- a lack of integrin engagement leads to the induction of cellular markers associated with myeloid differentiation
- GATA-2 expression in bone marrow stromal cells from chronic aplastic anemia was significantly lower than controls. Expression levels of GATA genes may influence hematopoiesis in BM microenvironment & relate to the pathogenesis/development of AA.
- data strongly suggest that GATA-2 mutations may play a role in acute myeloid transformation in a subset of CML patients
- Introducing GATA2 into microvascular endothelial cells resulted in dedifferentiation-like transcriptome reprogramming, with hematopoietic stem cell genes (such as ANGPT1) being up and endothelial genes (such as EPHB2) being down.
- Evi-1 promotes hematopoietic stem/progenitor expansion at the embryonic stage through up-regulation of GATA-2 and repression of TGF-beta signaling.
- Hypoxic regulation of Ang-2 is HIF-dependent and demonstrate that HIF-1alpha binds in human microvascular endothelial cells (HMVEC) to an evolutionary conserved Hypoxia-Responsive Element (HRE) located in the first intron of the Ang-2 gene.
- Gata-2 overexpression in AML was associated with a low percentage of blasts in bone marrow and males.