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Validated All-in-One™ qPCR Primer for RRM2(NM_001165931.1) Search again
Product ID:
HQP055675
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
C2orf48, R2, RR2, RR2M
Gene Description:
ribonucleotide reductase regulatory subunit M2
Target Gene Accession:
NM_001165931.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion.
Gene References into function
- Human ribonucleotide reductase M2 subunit gene amplification and transcriptional regulation in a homogeneous staining chromosome region responsible for the mechanism of drug resistance
- Characterization of the human ribonucleotide reductase M2 subunit gene; genomic structure and promoter analyses
- Wild-type p53 regulates human ribonucleotide reductase by protein-protein interaction with p53R2 as well as hRRM2 subunits.
- Sequences downstream of the transcription initiation site are important for proper initiation and regulation of this gene's transcription.
- Expression of antisense hRRM2 in PC3 cells led to decreased hRRM2 expression and resulted in greater sensitivity to UV than observed in wild-type PC3 cells.
- RRM2 overexpression is associated with gemcitabine chemoresistance in pancreatic adenocarcinoma cells. Suppression of RRM2 expression using RNA interference mediated by siRNA enhances the drug-induced cytotoxicity.
- Susceptibility differences to RR inhibitors between hRRM1 and hRRM2 may lead to a new direction in drug design for human cancer treatment.
- R2 subunitof RNR can substuitute for the function of p53R2 in providing dNTPs for DNA repair in cells lacking functional p53R2.
- RRM2 and p53R2 subunits share the same binding site on RRM1
- NF-kappaB is a key mediator of the invasive phenotypic changes induced by RRM2 overexpression.
- RR M2 protein may not have a critical role in the malignant potential of pancreatic cancer
- Ribonucleotide reductase subunit RRM2 mRNA expression in lung adenocarcinoma is associated with clinical outcome of docetaxel/gemcitabine therapy.
- RRM2 mRNA levels were assessed by quantitative PCR and correlated with response, time to progression and survival
- The redox property, structure, and function of hRRM2 and p53R2, are studied.