|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for CXCL1(NM_001511.3) Search again
Product ID:
HQP055552
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1
Gene Description:
C-X-C motif chemokine ligand 1
Target Gene Accession:
NM_001511.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
Gene References into function
- GRO-alpha levels in blood, quantities released from platelets ex vivo, and quantities released from SFFLRN stimulated platelets ex vivo were compared for preeclamptic and normal pregnancies.
- may play a role in the pathogenesis of endometriosis, possibly by chemoattraction and activation of neutrophils present in higher numbers in the peritoneal fluid of women with endometriosis
- mediates angiogenesis in Kaposi's sarcoma
- Eosinophils produce large amounts of the CXC chemokine GRO-alpha, which may be important during resolution of inflammation and may explain the interaction between eosinophils and certain tumors.
- The role of GROa in cultured nasal epithelial cells was studied for its ability to synthesize and deliver neutrophil chemotactic factors following TNF-alpha induction.
- Recombinant GROalpha induces articular chondrocyte hypertrophy and calcification through p38 MAP kinase and transglutaminase 2.
- expression of GRO-1 in 9 oral squamous cell carcinoma (OSCC) cell lines and 94 OSCC specimens; findings suggest a possible relationship between the expression level of GRO-1 and tumor progression
- This suggests that GRO-alpha plays a role in the infiltration of neutrophils into the lesional skin and in bulla formation in linear IgA bullous dermatosis.
- KC (CXCL1) mRNA is regulated by functionally independent AU-rich sequence motifs
- We propose that the concurrence of CXCR2 on oligodendrocytes and induced CXCL1 on hypertrophic astrocytes in MS provides a novel mechanism for recruitment of oligodendrocytes to areas of damage, an essential prerequisite for lesion repair.
- endothelin-1 (ET-1) induces CXCL1 and CXCL8 secretion in three human melanoma cell lines
- CXCL1 released from prostaglandin E2-treated carcinoma cells induces microvascular endothelial cell migration and tube formation in vitro.
- IL-1beta- and TNF-alpha-stimulated expression of GRO-alpha from airway smooth muscle is regulated by independent pathways involving NF-kappaB activation and ERK and JNK pathways
- LDL lipoprotein subunit L5 induces human umbilical vein endothelial cells (HUVEC) to express GRO-alpha.
- Gro-1 may be a therapeutic target as well as a diagnostic marker in ovarian cancer
- Coexpression of fibulin-1 with GROalpha abrogates key aspects of the transformed phenotype, including colonic tumor formation in a murine xenograft model.
- GROalpha led to p38 MAPK activation in chondrocytes cultured in micromass but not as a high-density monolayer. This caused the downstream triggering of chondrocyte hypertrophy and apoptosis/anoikis following concurrence of matrix degrading activity.
- CX3CR1 and CX3CL1 mediate heterotypic anchorage of foam cells to coronary artery smooth muscle cells in the context of atherosclerosis
- Inhibition of ERK decreased expression of Groa.
- PAR-2 plays a role in serine protease-mediated regulation of IL-8 and GRO-alpha in nasal epithelial cells and may be involved in the pathophysiology of rhinosinusitis.
- During treatment of ulceretive colitis with corticosteroids CXCL1/CXCL9 were decreased.
- rease of CXCL1 and -2 mediated by Curcumin is involved in the inhibition of metastasis in breast cancer cells.
- GRO-alpha may be a novel diagnostic marker for age-related pathology, including cancer.
- The production of GRO-alpha, IL-6 and IL-8 was shown to account for the ability of the HeLa cell culture medium to stimulate the migration of monocytes/macrophages, suggesting a key role for p38 MAPK and ERK1/ERK2.
- In addition to transcriptional up regulation of CXCL1, these primary cells exhibited the greatest IL-8 secretion and cell damage in response to stimulation with an acapsular strain of C. neoformans.
- GROalpha could be involved in atherogenesis and plaque destabilization, potentially contributing to inflammation, matrix degradation, and lipid accumulation within the atherosclerotic lesion.
- CXCL1 secreted by endothelial cells induces tumor cell invasion
- results suggest that CXCL1 modulates the invasive abilities of bladder cancer cells and this chemokine may be a potential candidate of urinary biomarker for invasive bladder cancer and a possible therapeutic target for preventing tumor invasion