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Validated All-in-One™ qPCR Primer for EFNB2(NM_004093.3) Search again
Product ID:
HQP055543
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EPLG5, HTKL, Htk-L, LERK5, ephrin-B2
Gene Description:
ephrin B2
Target Gene Accession:
NM_004093.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
Gene References into function
- expressed differentially in colon carcinoma and normal mucosa specimens and may play a role in progression of colon carcinoma
- Endogenous ephrin B2 from human umbilical artery endothelial cells activates a kinase that phosphorylates murine GST-ephrin-B1 cytoplasmic domain fusion proteins.
- role of ephrin-B2 in erythropoiesis
- Expression profile of this ligand of EPHB2 in gastric cancer
- The entire cytoplasmic domain of ephrin-B2 and its N-terminal fragment, residues 253-300, lack the ability to fold into a well-defined three dimensional structure and are particularly prone to aggregation.
- These data identify distinct propulsive and repulsive effector functions of endothelial ephrinB2 and EphB4 that mediate spatial positional signals during angiogenesis and vessel assembly.
- ephrin B2 is present in human retinal endothelial cells and vascular growth may be modulated in the retina through activation of the PI3K pathway.
- Overexpression of ephrin-B2 suppresses tumour cell growth and vascular function in an in vivo colon cancer model
- in hyperinflammatory lesions, ephrinB2 was predominantly expressed in macrophage-like cells and EphB4 in small venules; syntenin and syndecan-1 were up-regulated in EphB4-positive endothelial cells after stimulation with preclustered ephrinB2
- EphB4 and ephrin-B2 molecules, which have specific expression patterns during artery and vein development, respectively, were expressed in the placenta
- Ephrin B2 is an important novel factor in Kaposi sarcoma biology and a potential target for therapy.
- defined the expression patterns of human ephrinB2, EphB4, and EphB2 in normal vessels of neonates (i.e. umbilici) and adults and compared them with those in congenital venous malformations
- analysis of binding between the Tyr-phosphorylated human ephrinB2 and Nck2 SH2 domain
- ephrin-B signaling might represent a novel protective mechanism that promotes intestinal epithelial wound healing, with potential impact on epithelial restitution in Crohn disease.
- ephrin-B2 was found capable of rendering HeLa-USU cells permissive for Hendra virus and Nipah virus-mediated cell fusion as well as infection by live virus.
- ephrinB2 is a functional receptor for Nipah virus
- EphB4 and ephrin-B2 are instrumental in the establishment of a functional placental structure and of the utero-placental circulation.
- endothelial cell ephrinB2 expression is controlled by microenvironmental determinants rather than being an intrinsic endothelial cell differentiation marker
- Dll4 stimulation but not Jagged1 markedly induced ephrinB2 expression
- The results provide critical information on the EphB4*ephrinB2 protein interfaces and their mode of interaction, which will facilitate development of small molecule compounds inhibiting the EphB4*ephrinB2 interaction as novel cancer therapeutics.
- indicate that stimulated lymphocytes express EphrinB2, which has the potential to activate endothelial cells
- High ephrinB2 is associated with carcinogenesis and portal vein tumor thrombus formation in human hepatocellular
- demonstrates that Eph B4 and Ephrin B2 interaction may play an important role in the oncogenesis and angiogenesis of cervical carcinomas
- EphB/ephrin-B molecules play a role in restricting dental pulp stem cell attachment and migration to maintain Dthesse cells within their stem cell niche under steady-state conditions.
- Lubrol-RAFTs probably represent the platform for tumor-promoting ephrin-B2-integrin-beta1 interaction, which could become an interesting target for future antitumoral therapies
- EFNB2 gene expression may be a biological marker and a useful prognostic indicator in patients with oesophageal squamous cell carcinoma.
- Overexpression of ephrinB2 is associated with tumour advancement of ovarian cancers
- The ephrin-B2 ectodomain binds to EphB2 with a K(d) of 22.3 nM to form a tight complex in which the tip of the C-D loop and the C-terminus still remain largely flexible.
- expression of ephrinB2 in adult endothelial cells is up-regulated during arterial remodeling and controlled by cyclic stretch
- crystal structures of both Nipah and Hendra attachment glycoproteins in complex with human EFNB2
- Platelet-derived growth factor signaling through ephrin-b2 regulates hepatic vascular structure and function.
- polymorphisms in the EFNB2 gene do not appear to contribute in a substantial way to non-diabetic, diabetic or all-cause ESRD susceptibility in African-Americans
- MMP7 and MMP9-mediated cleavage of EphB2 is induced by receptor-ligand interactions at the cell surface and that this event triggers cell-repulsive responses
- We demonstrated limited Eph/Ephrin expression; notably, the only ligand highly expressed is EphrinB2 in Kaposi sarcoma
- EphB4 receptor activation by ephrin B2 in osteoarthritis subchondral bone could affect abnormal metabolism in this tissue by inhibiting resorption factors and their activities
- EB2 is not only necessary but also sufficient to allow the establishment of a productive NiV infection.