|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for DNMT3B(NM_001207055.1) Search again
Product ID:
HQP055494
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
FSHD4, ICF, ICF1, M.HsaIIIB
Gene Description:
DNA methyltransferase 3 beta
Target Gene Accession:
NM_001207055.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq].
Gene References into function
- DNMT1 and DNMT3b cooperate to silence genes in human cancer cells
- An essential role in cancer cell survival
- Overexpression of a splice variant is associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis
- the human de novo enzymes hDNMT3a and hDNMT3b form complexes with the major maintenance enzyme hDNMT1.
- The DNMT3B C46359T polymorphism is associated with promoter activity of a novel DNMT3B transcript, and associated with the risk of lung cancer in non-Hispanic whites.
- cloned and characterized the 5'-end of the mRNA and promoter regions
- a registry of all known ICF-causing mutations, DNMT3Bbase, was constructed. The structural principles of the pathogenic mutations based on the modelled structure and the analysis of chi angle rotation changes of mutated side chains are discussed.
- Over-expressed in squamous cell carcinoma of the mouth.
- DNMT3b plays an important role in neoplastic transformation
- DNMT3B is required for the methylation of L1 CpG islands on the inactive X chromosome.
- Overexpression of DNMT3B is associated with breast tumor
- DNMT1 plays a key role in methylation maintenance, DNMT3b may act as an accessory to support the function in ovarian cancer cells.
- DNMT3B co-purifies and interacts, both in vivo and in vitro, with several components of the condensin complex (hCAP-C, hCAP-E and hCAP-G) and KIF4A
- The DNMT3B C46359T polymorphism is statistically significantly associated with survival outcome in HNSCC patients.
- Down regulation of DNMT3b is associated with B-cell chronic lymphocytic leukemia
- Overexpression of DNMT3b4 is involved in human hepatocarcinogenesis, even at the precancerous stages, not only by inducing chromosomal instability but also by affecting the expression of specific genes
- The DNMT3B -283T > C polymorphism influences DNMT3B expression, thus contributing to the genetic susceptibility to lung cancer.
- two types of ICF patients that harbor different genetic characteristics; ICF type 1 is characterized by DNMT3B mutations and normal methylation of the alpha satellites; Type 2 lacks DNMT3B mutations and shows hypomethylation of the alpha satellites.
- DNMT3B was shown to have a very pronounced flanking sequence preference for human DNA methylation.
- The distribution of DNMT3B SNP in North China is distinct from that in Caucasians, but it cannot be used as a stratification marker to predict susceptibility and lymphatic metastasis of gasstric cardia adenocarcinoma.
- involvement of HPV infection in nonsmoking female lung tumorigenesis may be mediated, at least to a certain extent, through the increase of DNMT3b protein expression to cause p16INK4a promoter hypermethylation
- Data suggest that the DNMT3B TT genotype may be associated with an increased risk of prostate cancer.
- we observed that the increase (10%) of genomic DNA methylation in patients with alcoholism was significantly associated with their lowered DNMT-3b mRNA expression
- Transfection with the antisense DNMT3b gene eukaryotic expression plasmid can significantly reduce the expression level of the DNMT3b gene in the human biliary tract carcinoma cell line QBC-939.
- Dnmt3b plays an essential role at different stages of mouse development, and that Immunodeficiency, Centromeric instability and Facial anomalies missense mutations cause partial loss of function
- Dnmt3b-Dnmt3L interactions play an important role in the regulation of DNA methylation during mammalian development.
- DeltaDNMT3B is the predominant form of DNMT3B in non-small cell lung cancer.
- The relative distribution of three DNMT3B SNPs among a Taiwanese population can not be used as a stratification marker to predict either an individual's susceptibility to HNSCC and/or the likelihood of cervical metastasis of HNSCC.
- Expression of DNMT3B variants is common in nsn-small cell lung carcinoma and may play an important role in the development of promoter methylation.
- RAS oncogene induces RECK gene silencing through DNMT3b-mediated promoter methylation which may be useful in treatment of cancer metastasis
- Expression of DNMT3B was inversely correlated with that of p14ARF and p16INK4a. Results suggest that DNMT3B over-expression may be involved in the suppression or lower expression of p14ARF and p16INK4a observed in esophageal squamous cell carcinoma.
- The genes DNMT1, DNMT3A, and DNMT3B were over-expressed in the ectopic endometrium as compared with normal control subjects or the eutopic endometrium of women with endometriosis
- The p16 gene promoter was hypermethylated in pterygia, and this hypermethylation was strongly linked to expression of the positive expression of DNMT3b protein and to the suppression of p16 protein.
- results showed for the first the direct linkage between DNMT and zinc-fingers homeoboxes protein, leading to enhanced gene silencing by DNMT3B
- The DNMT3b 39179GT polymorphism may be a genetic determinant of adenocarcinoma of the colon, especially in younger Korean men.
- Results suggest that truncated DNMT3B proteins could play a role in the abnormal distribution of DNA methylation found in cancer cells.
- tobacco exposure induces the abnormal expression of SNCG in lung cancer cells through downregulation of DNMT3B
- DNMT1 plays a key role in maintenance of methylation, and DNMT3B may act as an accessory DNA methyltransferase to epigenetically silence CXCL12 expression in MCF-7 and AsPC1 cells
- Progressive up-regulation of the gene encoding DNMT3B was found in the colorectal adenoma-carcinoma sequence.
- We found clinically relevant levels of Hcy (0-500 microM) induced elevation of SAH, declination of SAM and SAM/SAH ratio and reduced expression of SAHH and MBD2, but increased activity of DNMT3a and DNMT3b affecting DNA methylation
- Early prenatal diagnosis of ICF syndrome by mutation detection.
- Expression of microRNA is inversely correlated with DNMT3B in non-small cell lung cancer.
- reveals novel functions for DNMT1 as a component of the cellular response to DNA damage, which may help optimize patient responses to this agent in the future
- the autocrine hGH-stimulated increases in DNMT3A and DNMT3B expression mediate repression of plakoglobin gene transcription by direct hypermethylation of its promoter and consequent phenotypic conversion of mammary carcinoma cells
- promoter DNA methylation can be differentially regulated and DeltaDNMT3Bs are involved in regulation of such promoter-specific de novo DNA methylation
- genotype and allelotype distribution in Chinese Han hepatocellular carcinoma patients was not significantly different from that in controls
- DNMT3B mutations in ICF syndrome lead to altered epigenetic modifications and aberrant expression of genes regulating development, neurogenesis and immune function.
- DNMT3B might not play a role in hypermethylation of many tumor suppressor genes during carcinogenesis of NPC
- DNMT3b overexpression is associated with human breast cancer
- DNMT3b was over expressed in gastric neoplasms.
- Polymorphisms in DNMT3B may be associated with colorectal adenoma risk in the context of low folate and methionine intake.
- There is no significant association between the DeltaDNMT3B C>T SNP and early onset CRC in HNPCC patients.
- Whereas overexpression of miR-148 results in decreased DNMT3b1 expression, short-hairpin RNA-mediated miR-148 repression leads to an increase in DNMT3b1 expression.
- DNMT3B 579 G>T promoter polymorphism does not have a role in susceptibility to esophageal carcinoma in Chinese
- Chromatin immunoprecipitation analysis of the BAG-1 promoter in DNMT1-overexpressing or DNMT3B-overexpressing colon cells show a permissive chromatin status assoscsiated with DNA binding of BORIS.
- DNMT3b may play an important role in modulating NSCLC patient survival and thus be useful for identifying NSCLC patients who would benefit most from aggressive therapy.
- It has been demonstrated that it may not be used as a stratification marker to predict the susceptibility to idiopathic thrombocytopenic purpura in Chinese population
- This large study provides reliable risk estimates for associations between DNMT3B variants and SCCHN risk in non-Hispanic whites
- The association between sequence variants of DNMT1 and 3B and mutagen sensitivity induced by BPDE supports the involvement of these DNMTs in protecting the cell from DNA damage.
- DNMT3b as a putative mediator of epigenetic control through histone modifications of gene expression in pituitary cells.
- DNMT1 and DNMT3b will increase their biological effects and have a synergistic effect on suppressing the growth of cholangiocarcinoma
- Results show that DNA methyltransferase 3B enhances polycomb protein 2-mediated transcriptional repression of FGFR3, and suggest that DNMT3B is a co-repressor of hPc2 in inducing transcriptional repression independent of DNA methylation.
- DNMT gene transcript expression was altered in several brains regions of suicides, including frontopolar cortex, amygdala, and paraventricular nucleus of the hypothalamus. Increased mRNA and protein expression was found in the frontopolar cortex.
- the DNMT3B -579G>T polymorphism may contribute to the genetic susceptibility to colorectal cancer.
- Decreased mRNA expression in peripheral blood mononuclear cells in patients with idiopathic thrombocytopenic purpura
- a negative association of DNMT3a and 3b expression with MDS disease risk
- role of deltaDNMT3B4/2 in regulating RASSF1A promoter-specific DNA methylation in non-small cell lung cancer.