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Validated All-in-One™ qPCR Primer for ATF3(NM_001674.3) Search again
Product ID:
HQP055430
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
-
Gene Description:
activating transcription factor 3
Target Gene Accession:
NM_001674.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors.
Gene References into function
- role of JNK-ATF3 pathway in apoptosis of vascular endothelial cells associated with hyperhomocysteinemia
- represses 72-kDa type IV collagenase expression by antagonizing p53-dependent trans-activation of the collagenase promoter
- An alternatively spliced isoform of transcriptional repressor ATF3 and its induction by stress stimuli.
- These results demonstrate that overexpression of Activating transcription factor 3 (ATF3) suppresses tumor necrosis factor-alpha-induced cell death of HUVECs, at least in part, through down-regulating the transcription of p53 gene.
- human ATF3 gene is one of the target genes directly activated by p53
- induction of ATF3 protein, a member of the ATF/CREB family of transcription factors, by ionizing radiation requires normal cellular p53 function
- ATF3 may function as a cytoprotective transcription factor in DOX-treated cardiac myocytes, at least in part, owing to downregulation of p53
- ATF-2 and ATF3 seem to play an important role in the protective response of human cells to ionizing radiation
- ATF3 isoforms have a role in the transcriptional regulation of the ASNS gene in response to nutrient deprivation
- Demonstrate the critical role of tyrosine phosphorylated ATF3 in IL-10 suppression of MMP-2 gene expression in primary human prostate tumor cells.
- ATF3-FL and C/EBPbeta act as transcriptional suppressors for the ASNS gene to counterbalance the transcription rate activated by ATF4 following amino acid deprivation
- The results suggest that I3C represses cell proliferation through up-regulation of NAG-1 and that ATF3 may play a pivotal role in DIM-induced NAG-1 expression in human colorectal cancer cells.
- this is a novel first report demonstrating that the expression of ATF3 occurs via early growth response gene-1 downstream of extracellular signal-regulated kinase1/2
- coordinated regulation of mRNA stability by HuR and AUF1 proteins contributes to the observed increase in ATF3 expression following amino acid limitation
- high expression of ATF3 is found in nearly all cases of cHL and is almost exclusively restricted to it
- t10,c12-CLA stimulates ATF3/NAG-1 expression and subsequently induces apoptosis in an isomer specific manner
- ATF3 is up-regulated in the penile skin tissues of boys with hypospadias, which suggests a role for this transcription factor in the development of this abnormality.
- gene mapped to chromosome 1.
- role in a novel mechanism used by interferon-induced serine/threonine protein kinase (PKR) to induce apoptosis
- Transcription factor Wilms' tumor 1 and ATF3 Transcription Factor are activated in Wilms' tumor and accelerate tumorigenesis.
- Initial increase in RNA pol II (RNA polymerase II) binding to the promoter and in the transcription rate from the ATF3 gene.
- results indicate that a major signalling pathway, the p38 pathway, plays a critical role in the induction of ATF3 by stress signals, and that ATF3 is functionally important to mediate the pro-apoptotic effects of p38
- expression of ATF-3 in hypoxic cells represses Id-1 and prevents their loss
- The tumor metastasis suppressor gene Drg-1 suppresses metastasis of prostate tumor cells by inhibiting the invasive ability of the cells via down-regulation of the expression of the ATF3 gene.
- ATF3 may have oncogenic properties in epithelial cells
- We found significant differences in gene expression, specifically with a group of genes, including ATF3, known to be responsive to estrogen or to interact with estrogen receptor.
- Coxsackievirus B3 (CVB3) infection markedly reduced ATF3 expression at mRNA and protein levels in parallel with p53 degradation, and preservation of p53 expression rescued CVB3 infection-induced ATF3 downregulation.
- ATF3 has a dichotomous role in cancer development.
- 17-alpha ethinyl estradiol upregulates ATF3 expression in human foreskin fibroblasts in vitro.
- Sp1 recruits ATF3, c-Jun, and STAT3 to obtain the requisite synergistic effect in neuronal injury through DINE neuronal injury-inducible gene
- ATF3 plays a protective role in renal ischemia-reperfusion injury and the mechanism of the protection may involve suppression of p53 and induction of p21
- ATF3 gene variants influence the risk of hypospadias
- ATF3 is a key mediator of KLF6-induced apoptosis in prostate cancer cells
- IFN-gamma inhibits matrix metalloproteinase expression by inducing expression of transcriptional repressor ATF-3, which then participates in feedback inhibition of Toll-like receptor-induced gene expression in primary human macrophages.
- both ATF3 and Smad were crucially and synergistically involved in down-regulation of Id-1, which regulated JNK phosphorylation in REIC/Dkk-3-induced apoptosis.