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Validated All-in-One™ qPCR Primer for CLDN7(NM_001185022.1) Search again
Product ID:
HQP055235
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CEPTRL2, CLDN-7, CPETRL2, Hs.84359, claudin-1
Gene Description:
claudin 7
Target Gene Accession:
NM_001185022.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells (Zheng et al., 2003 [PubMed 14502431]).[supplied by OMIM].
Gene References into function
- Loss of claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast, providing insight into the potential role of CLDN-7 in the progression and ability of breast cancer cells to disseminate.
- 2 forms of claudin-7, a full-length form with 211 AA residues and a C-terminal truncated form with 158 AA residues, are able to regulate the expression of a tissue-specific protein, prostate-specific antigen, in the LNCaP prostate cancer cell line.
- Loss of claudin-1 expression proved to be a strong predictor of disease recurrence and poor patient survival in stage II colon cancer.
- Claudin-1 and claudin-7 may play a significant role in tumor progression of cervical neoplasia and may represent useful markers for malignant transformation of cervical squamous cells.
- Overexpression of claudin-7 is associated with gastric tumorigenesis
- induction of claudin7 expression by ELF3 appears critical to the formation of the epithelial structures in biphasic synovial sarcoma
- Claudin tight junction proteins in endoscopy biopsy samples showed Barrett's metaplasia contains more claudin-2 and claudin-3 than found in normal esophageal mucosa, but markedly lower claudins 1 and 5, indicating very different tight junction barriers.
- When compared, adenocarcinomas and squamous cell carcinomas revealed significant differences in CLDN7 expression.
- claudin-7-associated EpCAM is recruited into (tetraspanin-enriched membrane microdomains) and forms a complex with CO-029 and CD44v6 that facilitates metastasis formation
- Results show show that claudin 7 expression changed with the gastric carcinogenic process and that this is implicated in cancer characteristics.
- Claudin-7 to be a candidate expression marker for distinguishing chromophobe renal cell carcinoma from other renal tumor subtypes, including the morphologically similar oncocytoma.
- Reduced claudin-7 expression correlates with loss of differentiation in thyroid neoplasms.
- CLDN-7 is significantly overexpressed in all main histologic types of epithelial ovarian cancer
- The role of extracellular loop domains of claudin-1 in determining tight junctions function, is studied.
- a reduced expression of the claudin-7 gene might lead to venous invasion and liver metastasis in colorectal cancer.
- Claudin-3 and claudin-7 expression in effusions independently predicts poor survival in ovarian cancer.
- claudin-7 overexpression promotes a loss of tumor cell polarization and contributes to tumorigenesis
- neither occludin nor claudin-7 expression was associated with clinicopathologic findings in patients with urothelial carcinoma of the upper urinary tract.
- Claudin-1 plays a crucial role in recruiting occludin to tight junctions, and occludin is involved in intercellular barrier function.
- Aspirin induces gastric epithelial barrier dysfunction by activating p38 MAPK via claudin-7.
- claudin-7 and claudin-8 have potential use as immunohistochemical biomarkers in the differential diagnosis of chromophobe renal cell carcinoma and oncocytoma
- Hepatocyte tight junctions-associated proteins occludin, claudin-1, and Zonula Occludens protein-1 (ZO-1) disappeared from the borders of adjacent cells in hepatoma cells harboring genomic hepatitis c virus replicons.
- claudin-1 expression is an independent prognosticator of shortened disease-specific patient survival in clinically relevant subgroups of clear cell renal cell carcinoma