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Validated All-in-One™ qPCR Primer for ARNT(NM_001197325.1) Search again
Product ID:
HQP055232
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ARNT1, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2
Gene Description:
aryl hydrocarbon receptor nuclear translocator
Target Gene Accession:
NM_001197325.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus.
Gene References into function
- The female reproductive tract expresses AHR and ARNT mRNA, and changes in expression at target sites in conditions such as endometriosis and uterine leiomyomas suggest a potential role for these factors in the pathogenesis of these conditions.
- SRC-1, NCoA-2, and p/CIP are capable of independently enhancing TCDD-dependent induction of a luciferase reporter gene by the AHR/ARNT dimer
- modification by SUMO-1 chiefly at Lys(245) within the PAS domain of this protein, both in vivo and in vitro
- experiments revealed a complex distribution of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator mRNAs and proteins in rat and human testis
- function as potent coactivator of estrogen receptor-dependent transcription
- estrogen receptor-mediated estrogen signalling is modulated by a co-regulatory-like function of activated AhR/Arnt, giving rise to adverse oestrogen-related actions of dioxin-type environmental contaminants
- formation of stable protein-DNA complexes by DR/Arnt and HIF-1alpha/Arnt heterodimers with their cognate DNA sequences requires Per/Arnt/Sim A domains
- nucleotide preference of the heterodimers of HLF and Arnt, and of AHR and Arnt
- Studies using a small interfering RNA to down-regulate Arnt protein expression revealed that TCDD-induced G(1) arrest is absolutely dependent on the Arnt protein.
- ER alpha-AHR-ARNT protein-protein interactions mediate estradiol-dependent transrepression of dioxin-inducible gene transcription
- Stat3 is required for both basal and growth signal-induced expression of HIF-1
- The role of ARNT/HIF1beta and altered gene expression in impaired beta cell function and the pathogenesis of human type 2 diabetes.
- Ainp2 enhances the 3-methylchloranthrene-induced activity in HepG2 cells, suggesting that Ainp2 plays a role in the Arnt-dependent function.
- Phosphorylation of Ser77 within the alternative exon of ARNT has a major influence on the activity of alternatively (Alt) spliced ARNT homodimers, but not an Alt ARNT heterodimer.
- In our cohort of patients, the polymorphism in codon 511 of the ARNT gene is not associated with RM.
- dioxin receptor is silenced by promoter hypermethylation in human acute lymphoblastic leukemia through inhibition of Sp1 binding
- for the bHLH.PAS transcription factors Dioxin Receptor and Arnt, the DR PAS A domain has a role in dimerization and affinity for an atypical E-box DNA sequence
- In hypoxia, HIF-alpha is stabilized and either dimerizes with HIF-beta to form transcriptionally active HIF for a hypoxia response, or it interacts with unrelated proteins, enabling convergence of HIF oxygen sensing with other signaling pathways.
- xenobiotic (TCDD) treatments of breast cancer cells containing reduced levels of BRCA1 cause the transcription factor ARNT to become unstable
- Allele and genotype frequencies of AHR and ARNT polymorphisms were similar between infertile men and controls.
- Overexpression of presenilin-1 increased HIF-1beta, suggesting that HIF is downstream of presenilin
- ARNT variants are unlikely to explain the linkage signal on chromosome 1q, but may alter insulin secretion in nondiabetic subjects
- identified hypoxia inducible factor 1beta (HIF1beta) as a TG2 binding partner
- The downregulation of ETS1 expression with small interfering RNA (siRNA) involves HIF1beta in regulating hypoxia-inducible genes.
- AhR may be a key molecule to regulate chromosome positioning.
- Hybrids of the bHLH and bZIP protein motifs display different DNA-binding activities in vivo vs. in vitro.
- Curcumin attenuates cytochrome P450 induction in response to TCDD by ROS-dependently degrading AhR and ARNT.
- crystal structures of the heterodimer formed by the C-terminal PAS domains from the HIF2alpha and ARNT subunits of the HIF2 transcription factor, both in the absence and presence of an artificial ligand.
- identification of ARNT as a CD30-interacting protein that modulated the activity of the RelB subunit of the transcription factor NF-kappaB; findings indicate that ARNT functions in concert with RelB in a CD30-induced negative feedback mechanism