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Validated All-in-One™ qPCR Primer for CD19(NM_001178098.1) Search again
Product ID:
HQP055058
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B4, CVID3
Gene Description:
CD19 molecule
Target Gene Accession:
NM_001178098.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Gene References into function
- REVIEW:lack OF CD19 expression in malignant plasma cells (myeloma cells) may contribute to proliferative advantage of the malignant cell clones.
- The physiologic role of eight CD19 tyrosines was examined in CD19-knockout mice expressing transgenic human CD19 constructs.
- Coligation of the B cell antigen receptor with the complement (C3)-binding CD21/CD19/CD81 costimulatory complex can enhance the escape of human B cells from Fas-induced death.
- CD19 is continuously and stably expressed on all stages of B lineage differentiation, and it is a reliable cell membrane marker for diagnosing B lineage ALL and an ideal target for antibody-targeting treatment of leukemia.
- each of these signaling proteins (Lyn, Vav, PLCgamma2, Grb2, and the p85 subunit of phosphatidylinositol 3-kinase) contains at least one Src homology 2 (SH2) domain that interacts directly with the phosphorylated CD19 cytoplasmic domain with high affinit
- Overexpression in these cells indicates diseaase progression in B=cell leukemia patients.
- The CD19 -499G>T polymorphism is associated with higher CD19 expression in B cells, and with susceptibility to systemic sclerosis.
- Umbiliccal cord blood-derived CD19-specific T cells after cord blood transplantation can reduce the incidence of CD19+ acute B-cell leukemia relapse.
- CD19 is not always strongly expressed in B-cell neoplasms. Furthermore, the lymphocytic and histiocytic (L&H) cells of lymphocyte predominant Hodgkin's disease (which express most B-cell-associated markers) commonly lack CD19.
- This suggests that CD19 overexpression may promote anergic B cells to escape tolerance by converging with B Cell Receptor independent pathways, thereby rendering these B cells hyper-responsive to innate signaling.
- Mutation of the CD19 gene causes a type of hypogammaglobulinemia in which the response of mature B cells to antigenic stimulation is defective.
- lipid microdomain disruption and silencing of CD19 directly impacts on CD38's ability to mediate Ca(2+) fluxes, while leaving its surface expression unchanged
- These findings extend the mutation spectrum of the CD19 deficiency to four, and confirm the homogeneity of the CD19 deficiency as a unique type of CVID.
- Of 9 CVID patients...No mutations of SAP, ICOS, TACI, BAFFR, and CD19 were identified
- relative frequency of CD19 and/or CD56 expression in acute myeloid leukemia (AML) with t(8;21) was significantly higher than those without this translocation and co-expression of these two antigens may serve as the surrogate markers for AML with t(8;21)
- B-lineage commitment can occur before the expression of B220 and CD19
- Data report the first hematopoietic mHag presented by HLA class II molecules to CD4(+) T cells, which is encoded by a single-nucleotide polymorphism in the B cell lineage-specific CD19 gene.