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Validated All-in-One™ qPCR Primer for TFRC(NM_001128148.2) Search again
Product ID:
HQP054753
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90
Gene Description:
transferrin receptor
Target Gene Accession:
NM_001128148.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- gene coding and flanking regions were sequenced and examined for mutations that might modulate the iron burden of individuals harboring the common mutant hemochromatosis HFE genotype or cause hemochromatosis independent of mutations in the HFE gene
- mutational analysis of the transferrin receptor reveals overlapping HFE and transferrin binding sites
- location was observed on or near the cell surface suggesting it might participate in surface membrane transport of iron
- Analysis of TFRC1 genotypes and HFE gene mutations in French porphyria cutanea tarda (sPCT)patients revealed that, independently from HFE gene mutations, an association was found between the IVS4+198 T allele in the TFRC1 gene and sPCT patients.
- a biological marker of erythropoiesis: blood levels in chronic hemodialysis patients
- Circulating levels are affectd by endurance training along with other indicators of iron status in female athletes.
- Results suggest that transferrin receptor shedding is constitutively mediated by a member of the metalloprotease family known as ADAM (for a disintegrin and metalloprotease) that is inhibited by tumor necrosis factor alpha protease inhibitor-2 (TAPI-2).
- Multiple, conserved iron-responsive elements in the 3'-untranslated region of transferrin receptor mRNA enhance binding of iron regulatory protein 2
- membrane-associated forms of neutrophil elastase and cathepsin G may be involved in alternative transferrin receptor shedding in U937 cells
- determination of transferrin recycling pathway requiring phosphatidylinositol 3-kinase activity
- TfR and TfR2 have similar cellular localizations in K562 cells and coimmunoprecipitate to only a very limited extent. Western analysis of the receptors under nonreducing conditions reveals that they can form heterodimers.
- TfR1 polymorphisms bore no detectable relation to disease severity or response to therapy.
- human transferrin receptor recognizes a complex of Yb3+-transferrin which is a possible pathway for Yb3+ accumulation in cells
- Association between IgA deposits and CD71 expression and their co-localization in mesangium provide strong evidence that CD71 is major IgA receptor on mesangial cells.
- presence in blood serves as a diagnostic indicator of hemolytic anemia
- The concomitant presence of Abeta 1-40 fragment and of IL1beta or TNFalpha caused an increase in the percentage of CD71 positive cells
- in beta-thalassemic patients, significant reduction of CD49d, CD29 and CD71 antigen expression was found in peripheral blood nucleated red cells
- analysis of ligand recognition by the human transferrin receptor
- Reductive release of iron from transferrin, which binds Fe2+ very weakly, is physiologically feasible, a further indication that the transferrin receptor is more than a passive conveyor of transferrin and its iron.
- Increased expression of CD71 antigen is associated with melanoma
- MPP(+)-dependent aconitase inactivation, Tf-iron uptake, and oxidant generation result in the depletion of intracellular tetrahydrobiopterin, leading to the uncoupling of nNOS activity
- Study proposes mechanism of interaction of transferrin with its receptor based on at least two different TFR species: the TFR species found in the neutral media of biological fluids, and the acidic species found in the mildly acidic media of the endosome
- analysis of the structure of TfR-Tf complex explains differences in the iron-release properties of free and receptor bound Tf
- Transferrin receptor 1 is a gatekeeper for regulating iron [review].
- Toxoplasma gondii infection resulted in increased activity in the iron response protein IRP1, which, in this state, stabilizes transferrin receptor mRNA from degradation.
- monocyte-derived dendritic cells express another IgA receptor (IgA-R), the transferrin receptor (TfR)
- utility of sesrum TfR outcomes for the detection of iron deficiency during early lactation.
- Transferrin receptor (TfR) and hemochromatosis factor, as well as TfR and DMT1 interact in placental trophoblast cells
- The effects of mutating the liganding residues in the two lobes of transferrin and the subtle indications of cooperativity between lobes point to the importance of the transferrin receptor in effecting iron release from the C-lobe.
- functional cooperation between pIgA1 and TfR for IgA1 deposition and mesangial cell proliferation and activation
- Aluminum interference with transferrin receptor-mediated iron incorporation might trigger the upregulation of non-transferrin bound iron uptake.
- TfR1 expression is attenuated in a cell-density-dependent manner in human lung cancer H1299 cells and in murine B6 fibroblasts as the result of a marked decrease in mRNA content.
- Soluble transferrin receptor is an additional parameter to ferritin for the diagnosis of iron-deficiency anemia
- hypothesis that one molecular mechanism by which 11q23 deletions confer a poor prognosis in CLL is via increased TfR expression secondary to ATM loss, resulting in the increased cellular iron import, and hence increased capacity for malignant growth
- Iron binding C- and N-lobes of Tf sequester iron as a function of complex formation; these structural changes promote tighter binding of the metal ion and facilitate efficient ion transport during endocytosis.
- soluble transferrin receptor release is directly regulated by binding of its ligand ferritransferrin
- These findings provide a molecular basis for increased TFRC1 expression in human tumors, illuminate the role of TFRC1 in the c-Myc target gene network
- mechanism of iron release from the N-lobe and C-lobe of serum transferrin in interaction with intact transferrin receptor 1 at 4.3< or =pH< or =6.5
- Serum transferrin receptor concentrations were measured in 151 pairs of women with HIV infections were associated wiwth mortality.
- Increased expression for transferrin receptor mrna is associated with esophageal squamous cell carcinoma
- HFE and TFR2 interact in cells; this interaction is not abrogated by disease-associated mutations of HFE and TFR2; and that TFR2 competes with TFR1 for binding to HFE
- A positive correlation between BMI and sTfR concentration was detected.
- Expression from the transferrin receptor gene in endothelium requires the activity of both TFIID and Sp3, but whether the gene is transcribed in different endothelia, is related to the balance between activating and suppressive forms of YY1.
- we show that EGF relocates to the cell centre in a dynein-dependent fashion, concomitant with the sorting away of transferrin receptor, although it remains in Rab5-positive early endosomes.
- data indicate that TfR1 is a cellular receptor for New World haemorrhagic fever arenaviruses
- Both insulin sensitivity and glucose tolerance status are significantly associated with serum transferrin receptor concentrations.
- transferrin receptor and CD9, CD81, and CD9P-1 are differentially sorted into exosomes after TPA treatment of K562 cells
- provides an estimate of the serum transferrin receptor reference intervals in African children using the Ramco Laboratories, Stafford, TX assay kit
- Hypoxia has dual effect on the expression of TfR in human melanoma A375 cell line.
- the TfR2/HFE and TfR1/HFE interactions are distinct.
- by driving membrane tubulation, SNX4 coordinates iterative, geometric-based sorting of the TfnR with the long-range transport of carriers from early endosomes to the ERC
- comparison of the abilities of TfR1 orthologs from different species to support arenavirus entry found that the human and feline receptors were able to enhance entry of the pathogenic strains, but that neither the murine or canine forms were functional.
- These results indicate that the alpha(6)(bri)/CD71(dim) subpopulation enriched corneal epithelial stem cells.
- CD71 may have a role in the pathogenesis of celiac disease: secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor
- PtdIns(4,5)P(2) hydrolysis blocked transferrin receptor endocytosis and led to a marked increase in the concentration of transferrin receptors in the plasma membrane.
- Highly expressed TfR1 and ferritin in CD68-positive macrophages were significantly associated with development and severity of human carotid plaques, smoking, and patient's symptoms.
- Plasma ferritin and transferrin receptor concentration can provide an indication of body iron metabolism.
- TFR1 binds iron-loaded serum apotransferrin and allows its internalization into the cytoplasm.
- placental expression of TfR1 (transferrin receptor) mRNA increased significantly in mild anemia and decreased near to the normal in moderate anemia .
- In normal B cells, transcriptional mechanisms exert a critical control over TfR1 and TfR2 expression, whereas in CLL post-transcriptional mechanisms seem to play a complementary and perhaps more important role
- NSC306711 inhibits iron uptake from the Tf-TfR pathway by inducing internalization and degradation of unoccupied Tf receptors through an unexpected endocytic pathway
- Serum transferrin receptor (TFR) is not a decisive parameter that can be utilized alone in discriminating the border-line situations between normal and beta thalassemia carriers or storage iron deficiency in children
- The interrelationships between sTfR1 and prohepcidin or between ferritin and hepcidin suggest that ferritin- and sTfR1-sensed hepcidin conversion system exist in human body and maybe regulated at the post-translational level.
- A general mechanism for the interaction of five metal-loaded Transferrins [Fe(III), Al(III), Bi(III), Ga(III) and Co(III)] with transferrin receptor, is reported.
- miRNA (miR-320) contributes to downregulation of TfR-1 surface expression characteristically seen during HL-60 monocytic differentiation.