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Validated All-in-One™ qPCR Primer for CHRNA7(NM_000746.5) Search again
Product ID:
HQP054678
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CHRNA7-2, NACHRA7
Gene Description:
cholinergic receptor nicotinic alpha 7 subunit
Target Gene Accession:
NM_000746.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. [provided by RefSeq].
Gene References into function
- Changes in nicotinic acetylcholine receptor subunits expression in brain of patients with Down syndrome and Alzheimer's disease.
- Results suggest that the CHRNA7 may play a role in schizophrenia in these families.
- 3-Mb map of 15q13-q14 showing that CHRFAM7A is part of a large segmental duplication in the opposite orientation to CHRNA7 and revealing several other duplications
- Some properties of human neuronal alpha 7 nicotinic acetylcholine receptors fused to the green fluorescent protein
- single-channel properties of human acetylcholine alpha 7 receptors are altered by fusing alpha 7 to the green fluorescent protein
- The increasing levels of the alpha7 nicotinic acetylcholine receptor during the first trimester support the important role of nAChRs for the development of the central nervous system.
- Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes.
- The positional candidate gene CHRNA7 at the juvenile myoclonic epilepsy locus (EJM2) on chromosome 15q13-14.
- results strongly suggest that a human sperm alpha7 subunit-containing nicotinic acetylcholine receptor plays a role in the recombinant human ZP3-initiated acrosome reaction
- ACh signaling through alpha7 nAChR channels controls late stages of keratinocyte development in the epidermis by regulating expression of the cell cycle progression, apoptosis, and terminal differentiation genes and mediated, by transmembrane Ca2+ influx
- the -2 bp deletion within the CHRNA7-like gene is a risk factor for P50 sensory gating deficit in auditory evoked potentials
- the G23-N46 portion of the alpha7 N-terminal region may contribute to receptor homooligomerizations
- In the developing human spinal cord, neuronal nicotinic receptors are expressed during important embryonic periods.
- An increase in the number of astrocytes expressing alpha7 immunoreactivity was observed in Alzheimer Disease compared with age-matched controls. A similar increase was not evident in Lewy Body Dementia.
- An extra cysteine residue in the N-terminal extracellular domain, a unique feature of the alpha7 subunit, appears to have neither positive nor negative consequences in the formation of functional cell surface receptors.
- alpha 7-Nicotinic acetylcholine receptors alpha 7-nAChRs play important roles in chemical and electrical signaling, and perhaps in neurite outgrowth, synaptic plasticity, and neuronal death/survival.
- CHRNA7 subunit is expressed in the soma of the majority of pyramidal cells, with fairly consistent immunoreactivity observed throughout the different regions of the hippocampus.
- significant decrease of the alpha4 and the alpha7 nicotinic acetylcholine receptor subunit in cortices of Parkinson patients which turns out to be similar to recent findings in Alzheimer patients.
- Promoter region of the CHRNA7 gene as risk factors for the P50 inhibitory deficit in sschizophrenia.
- expression of CHRNA7 is decreased in schizophrenia
- The -2 bp polymorphism or a nearby polymorphism of the NACHR alpha7 subunit may play a role in the pathogenesis of Bipolar Disorder.
- Reduced gene expression of the alpha4beta2 nicotinic receptor in the cerebral cortex is a major feature of the neurochemical pathology of autism, whilst post-transcriptional abnormalities of both this and the alpha7 subtype are apparent in the cerebellum
- an association between the homozygous 113 bp allele of the alpha-7-nicotinic receptor and smoking risk in schizophrenics
- shows that the most important interactions occur between the residues V12-K28 from the peptide and the loop C of the receptor
- kinetic and solubility studies on human alpha7 acetylcholine receptor expressed in yeast
- direct evidence suggesting that the M5 muscarinic receptor gene combined with the alpha7-nicotinic receptor gene may be linked to schizophrenia
- The number of astrocytes labeled alpha7 antibodies was increased in most areas of the hippocampus and entorhinal cortex in Alzheimer's disease.
- alpha3 and alpha7 nicotinic acetylcholine receptors regulate keratinocyte chemokinesis and chemotaxis
- ric-3 appears to be necessary for proper folding and/or assembly of alpha7 receptors in HEK293 cells
- Mutations in the human alpha 7 neuronal nicotinic receptor subunit gene promoter that are associated with schizophrenia were not found in a population of 249 unrelated Han Chinese schizophrenic patients.
- The alpha7 nicotinic receptor protein is expressed in fewer cell lines, and the tobacco carcinogen NNK increases alpha7 nicotinic receptor protein levels.
- biophysical analysis of the gating mechanism of human alpha7 nicotinic acetylcholine receptor
- Results indicate that the alpha7 nicotinic receptor subunit may mediate some aspects of nicotine dependence.
- hRIC-3 can act as a specific regulator of alpha7 and 5-HT3 expression at different levels by increasing the number of mature receptors and facilitating its transport to the membrane
- The gating motion of the human nicotinic acetylcholine receptor (nAChR) alpha7 was investigated with normal mode analysis (NMA) of two homology models.
- Two alleles showed both familial transmission disequilibrium and population-wide association with schizophrenia. Variations in the frequency of SNPs in CHRNA7, the alpha-7-nicotinic acetylcholine receptor subunit gene at 15q14, were found in each group.
- Review cites the role of nAChR alpha 7 as an essential component of the cholinergic anti-inflammatory pathway, because activation of the receptor prevents cytokine release.
- Incorporation of beta3 into neuronal nicotinic receptors other than alpha3beta4 has a powerful dominant-negative effect. This raises the possibility of a novel regulatory role for the beta3 subunit on neuronal nicotinic signaling in CNS.
- Placental expression of alpha7 nAChR is increased in severe preeclampsia placentas when compared with normal placentas.
- The Ras/Raf-1/MEK1/ERK cascade culminates in nicotine up-regulated expression of the gene encoding STAT-3, whereas recruitment and activation of tyrosine kinase JAK-2 phosphorylates it.
- These data indicate that the muscle-type nAChR, rather than the alpha7 type, is highly expressed in NSCLC and leads to downstream activation of the p44/42 MAPK pathway
- A comparison of alpha7 nAChR levels in lymphocytes from Alzeimer disease patients and control subjects.
- Description of a fully human chimeric receptor (h-alpha7/5HT3A), which is characterized by desensitization, and recovery kinetics that deviate from the human WT alpha7.
- Of the nAChR subtypes studied, we have found that alpha7 receptors are very responsive to nornicotine (EC50 approximately 17 micromol/L I(max) 50%, compared with acetylcholine (ACh)
- Amyloid beta1-42 did neither inhibit the specific binding of alpha7nAChR ligands to rat brain homogenate or slice preparations, nor did it influence the activity of alpha7nAChRs expressed in Xenopus oocytes.
- In hippocampus from wild control mice, nicotine decreased LDH release evoked by Oxygen glucose deprivation plus re-oxygenation but failed to exert neuroprotection in alpha7 knockout mice.
- Resuts show that urothelium carries multiple cholinergic receptor subtypes, with predominant expression of M2R, M3R and alpha7-nAChR and suggest that this layer-specific distribution serves to stratify cholinergic regulation of urothelial function.
- Alpha7 nAChR may play a significant neuroprotective role by enhancing cleavage of APP by alpha-secretase, regulating signal transduction, improving antioxidant defenses and inhibiting the toxicity of Abeta, which is connected with the pathogenesis of AD.
- data reveal the contributory role alpha7-nAChR and beta-adrenoceptors in the tumorigenesis of colon cancer cells and partly elucidate the carcinogenic action of cigarette smoke on colon cancer
- Our data demonstrate that alpha7 transcription is altered in several ways in SZ, suggesting that transcription-level mechanisms could account in part for the impaired cholinergic neurotransmission observed in this disease.
- The results also reveal direct coupling between a twisting motion of the extracellular domain and dynamic changes of M2.
- T cells express alpha7-nAChRs that are critical in increasing [Ca(2+)](i) in response to nicotine.
- the molecular mechanism of the association between NRG1 risk alleles and schizophrenia may include down-regulation of nAChR alpha7 expression
- This study suggests the alpha7 subunit as one of the players in nicotine withdrawal, but not in nicotine tolerance or basal anxiety-like behavior.
- There was no evidence of association between schizophrenia and CNR1 (OR=0.97, 95% CI 0.82-1.13) or CHRNA7 (OR=1.07, 95% CI 0.77-1.49) genotypes, or of interactions between tobacco use and CHRNA7, or cannabis use and CNR1or COMT genotypes.
- discussion of the role of variability in the genes that encode the two major brain-expressed alpha subunits, alpha4 and alpha7, in modulating behavior, physiology and disease risk in both humans and mice [review]
- Three blocks of high linkage disequilibrium and low haplotype diversity were identified. The block 1 TCC haplotype was significantly associated with reduced odds of Alzheimer's disease (p = 0.001) and was independent of APOE status.
- NB alpha7 nAChR expression may serve as a marker for the progression of Alzheimer disease
- M1 muscarinic and alpha7 nicotinic receptors have roles in inhibition of pemphigus acantholysis
- The consistency of docking results and the agreement with the experimental data afford an encouraging validation for the proposed model and emphasize the soundness of such a fragmental approach to model any transmembrane protein.
- These findings provide experimental evidence that nicotine effects an improvement in impaired memory, and that this improvement is associated with an increase in nAchRalpha7.
- There may be association between CHRNA7 gene polymorphisms and schizophrenia, the variant allele T in rs2337980 may have a protective effect to schizophrenia.
- Environmental tobacco smoke and nicotine up-regulated the levels of alpha5 and alpha7 expression in a time-dependent fashion
- this recombinant cell system is useful for characterization of alpha7 nAChRs
- alpha7-subunit is constantly found in NE cells in normal lungs. In tumors, its expression is significantly higher in small-cell lung carcinoma than in carcinoid tumor (p=0.009)
- The RIC-3e protein is functionally active and enables surface expression of mature alpha7-nAChRs in cell lines not otherwise permissive for the expression of this receptor.
- plays important roles in attention, memory, and cognition and participate in the pathogenesis of several neuropsychiatric disorders. (review)
- These results showed that alpha7-nAChR plays an important role in non-small cell lung cancer cell growth and tumour progression as well as in cell death.