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Validated All-in-One™ qPCR Primer for IL6R(NM_000565.3) Search again
Product ID:
HQP054646
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD126, HIES5, IL-1Ra, IL-6R, IL-6R-1, IL-6RA, IL6Q, IL6QTL, IL6RA, IL6RQ, gp80
Gene Description:
interleukin 6 receptor
Target Gene Accession:
NM_000565.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.
Gene References into function
- Shedding of the interleukin-6 (IL-6) receptor (gp80) determines the ability of IL-6 to induce gp130 phosphorylation in human osteoblasts.
- Serum sIL-6R levels reflect proliferative kinetics of the progenitors after stimulation by chemotherapy plus granulocyte colony-stimulating factor.
- the soluble interleukin-6 receptor isoform induces major changes in gene expression profile of human hepatoma cells
- Proteolysis of interleukin-6 receptor (IL-6R) by Porphyromonas gingivalis cysteine proteinases (gingipains) inhibits interleukin-6-mediated cell activation.
- Human bone marrow-derived mesenchymal cells do not differentiate into the osteogenic lineage until Gp130 is activated by soluble interleukin-6 receptors and interleukin-6.
- combined measurements of serum interleukin-6 soluble receptor and interleukin-6 may be helpful in identifying patients with untreated hyperparathyroidism who are more likely to experience bone loss at the total femur
- results show for the first time that interleukin-6 (IL), in the presence of its soluble receptor (sIL-6R), induces activation of JAK1, JAK2, and STAT1/STAT3 proteins in bovine articular chondrocytes
- soluble proteins of tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-6 receptor subunit gp80 (sIL-6R gp80), as markers of multiple sclerosis
- X-ray structure (2.4 A) of the IL-6R ectodomains shows the N-terminal strand of the D(1)domain is disulfide-bonded to domain D(2). The head-to-tail packing of 2 closely associated IL-6R molecules may be the configuration of the physiological IL-6R dimer.
- There is no significant effect of IL-18 on the release of both soluble receptors of IL-6.
- Induction of VEGF seems to be regulated by the extent of the IL-6 receptor expression on pancreatic cancer cells.
- only in a subset of both benign and malignant thyroid nodules the interleukin-6/interleukin-6 receptor signal could be induced by the CD30 ligand/CD30
- IL-6r and TNF-alpha increase in parallel to VEGF and FGF-2 with increasing stage of multiple myeloma.
- identification of existence as a preformed dimer in plasma membrane
- IL-6 receptor binds to CTNF and is involved in neuroprotection
- type 1 tumor necrosis factor receptor shedding aminopeptidase regulator(ARTS-1) promotes shedding of two cytokine receptor superfamilies, type I cytokine receptor superfamily (interleukin-6Ralpha) and tumor necrosis factor receptor superfamily (TNFR1)
- might also be valuable biochemical marker in the evaluation of oocyte maturation
- IL-6 derived from blood during breakdown of blood-retinal barrier and produced by retinal pigment epithelial(RPE) cells and hematogenous soluble interleukin-6 receptor cause RPE proliferation.
- a structural model, derived from 3.65 A resolution crystallographic data of the complex between IL-6, the extracellular binding domains of IL-6R, and the extracellular activation and binding domains of gp130 is presented
- cholesterol depletion triggers shedding of the human interleukin-6 receptor by ADAM10 and ADAM17
- IL-15 alone, similarly to IL-18, has no significant ability for regulation of both soluble IL-6 receptors, sIL-6R and sgp130, released by human neutrophils.
- IL-6R alpha and IGF-I receptors exist on the plasma membrane in close proximity, facilitating the efficient assembly of 2 receptors in response to IL-6 in myeloma
- In prostatectomy patients, preoperative plasma levels of TGF-beta(1) and IL-6sR are associated with metastases to regional lymph nodes and disease progression.
- Serum pro-inflammatory cytokine IL-6 or TNF-alpha concentrations were neither increased in subjects with impaired glucose tolerance nor closely correlated with the components of the metabolic syndrome.
- Study using normal peritoneal mesothelial cells and inflammatory exudates reveals the comparable ability of two types of soluble IL-6 receptor isoforms to regulate neutrophil recruitment.
- IL-6 and sIL-6R may contribute to the pathogenesis of endometriosis-associated infertility
- NMR structure of the carboxyl terminal domain of the cytokine binding module of the interleukin-6 receptor
- Butyrate acts by inhibiting IL-6-induced interleukin 6 receptor expression in colorectal carcinoma cells.
- Detailed pathway analysis revealed that BM stromal cells stimulate STAT3 via the IL-6R, and MEK1/ERK1 pathways, via IL-6R-independent mechanisms
- Data describe the relationship between interleukin-6 and interleukin-6 soluble receptor in stroke patients.
- Asp358Ala polymorphism of the IL6R associates with type 2 diabetes in Danish whites.
- Variants are associated with Type II diabetes and diabetic nephropathy.
- the soluble form of IL-6 receptor is expressed on normal human platelets
- IL-1beta- and TNFalpha-mediated induction of IL-6R shedding in osteoblast-like cells is at least partly dependent on tumor necrosis factor alpha-converting enzyme activation.
- co-expression of IL-6 and its specific receptor (IL-6Ralpha) in keloid fibroblasts that points to the existence of an IL-6 autocrine loop in these cells.
- A crystal structure of the ligand-binding domains of gp130 in complex with human interleukin-6 (IL-6) and its a-receptor (IL-6Ralpha).
- Airway epithelial cell IL-6 and soluble IL-6 receptor alpha participate in inflammatory trans-signaling in response to bacterial pathogens.
- Production of IL-6 and its soluble receptors by stimulated peripheral blood monocytes appears to be inversely correlated with serum cholesterol levels in hemodialysis patients.
- CD30-L/CD30 and IL-6/IL-6R systems could play a major role in the pathogenesis of Graves'disease (GD), but the expression of CD30L/CD30 and IL-6/IL-6R in Hashimoto's thyroiditis (HT) suggests that Th2 mechanisms are involved in tissue damage.
- Tax-mediated regulations of IL-6R and sIL-6R observed in HTLV-I-associated disorders may contribute to proliferation of HTLV-I-infected T cells through activation of inducible STAT3
- High pretransplant sIL-6R plasma levels are associated with an increased risk of acute tubular necrosis (ATN) and might contribute to the development of ATN early posttransplant
- The selective increase in sIL-6R implicates an enhanced trans-signaling capacity whereby sleep distinctly widens the profile of IL-6 actions, enabling an integrated influence on brain and peripheral organs
- physical evidence of the stabilization of vIL-6-induced gp130 signaling complexes by gp80
- Anti-IL-6R antibody abrogated the inhibited proliferation and increased invasion induced by IL-6.
- blockade of IL-6 receptor following trauma-hemorrhage appears to be a novel and effective adjunct for improving organ and cell function
- using admixture mapping, we found a significant association of interleukin 6 soluble receptor (IL-6 SR) with European ancestry on chromosome 1 (LOD 4.59), in a region that includes the gene for this receptor (IL-6R)
- administration of IL-6 could activate receptor gp80/gp130 signaling pathways including downstream extracellular signal-regulated kinase 1/2 and STAT3 phosphorylation in EPCs.
- The aim of the study is to define IL-6 target gene regulation in cultivated human detrusor smooth muscle cells.
- A higher gene expression of CRP, IL-6, and both IL-6 membrane receptors in subcutaneous samples of inflamed patients than in healthy controls.
- A common variant of the IL-6r gene results in major changes in IL-6r and IL-6 serum levels, but with no apparent effect on gp130 levels or on inflammatory status in the general population.
- IL6R genotypes were not significantly associated with the risk of type 2 diabetes.
- We hypothesized that polymorphisms in inflammatory response genes contribute to the increased susceptibility to acute otitis media.The IL6-174 G/G genotype was overrepresented in children with acute otitis media when compared with controls
- As a mediator of interleukin-6 action, the interleukin-6 receptor gene is involved in the determination of body mass index and bone mineral density.
- Interleukin-6 (IL-6) and/or soluble IL-6 receptor down-regulation of human type II collagen gene expression in articular chondrocytes requires a decrease of Sp1.Sp3 ratio and of the binding activity of both factors to the COL2A1 promoter
- IL-6 and IL6-R SNPs were analyzed for associations with preterm birth.
- Production of sIL-6R by autoreactive CD4 T cells may contribute to their role in the development of autoimmune disease by conferring IL-6-responsiveness to cells lacking IL-6R such as synoviocytes.
- the study suggest a small but significant contribution of the IL-6R polymorphism to susceptibility to schizophrenia in a Chinese population
- Concluded that IL-6/sIL-6R enhances cathepsin B and L production via IL-6/sIL-6R-mediated Cav-1-JNK-AP-1 pathway in human gingival fibroblasts.
- These data suggest the involvement of IL-6R in melanoma development.
- Serum IL-6 and sIL-6R markedly increased after tocilizumab administration in both RA and Castleman disease.
- Results show that the designer fusion protein H-IL-6, consisting of IL-6 and its specific receptor IL-6R-alpha, but not IL-6 alone, mediates both neuro- as well as gliogenesis.
- sIL-6R levels <2.5 x 10(4) pg/mL were also associated with a significantly worse event-free survival
- No significant association was found between +48892 IL-6R or +24013 IL-6R- polymorphisms and nationality or in Behcets disease or health controls.
- Cross talk of signals between EGFR and IL-6R through JAK2/STAT3 mediate epithelial-mesenchymal transition in ovarian carcinomas.