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Validated All-in-One™ qPCR Primer for AHR(NM_001621.4) Search again
Product ID:
HQP054627
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
RP85, bHLHe76
Gene Description:
aryl hydrocarbon receptor
Target Gene Accession:
NM_001621.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq].
Gene References into function
- polymorphisms that result in loss of CYP1A1 induction
- Phylogenetic analysis shows that AHRR, AHR1, and AHR2 are distinct genes, members of an AHR gene family; these three vertebrate AHR-like genes descended from a single invertebrate AHR
- The female reproductive tract expresses AHR and ARNT mRNA, and changes in expression at target sites in conditions such as endometriosis and uterine leiomyomas suggest a potential role for these factors in the pathogenesis of these conditions.
- Aryl hydrocarbon receptor/dioxin receptor in human monocytes and macrophages
- Functional involvement of the Brahma/SWI2-related gene 1 protein in cytochrome P4501A1 transcription mediated by the aryl hydrocarbon receptor complex
- Arg554Lys was investigated and no association with micropenis was found.
- SRC-1, NCoA-2, and p/CIP are capable of independently enhancing TCDD-dependent induction of a luciferase reporter gene by the AHR/ARNT dimer.
- Two parallel pathways mediate cytoplasmic localization
- The silencing mediator of retinoic acid and thyroid hormone receptors can interact with the aryl hydrocarbon (Ah) receptor but fails to repress Ah receptor-dependent gene expression.
- functional AhR are present in endometrial and endometriotic stromal cells and that TCDD up-regulates the expression of RANTES, providing a possible mechanistic link between dioxin exposure and chemokine expression in endometriosis.
- Polymorphisms that regulate the phenotype of AHR-mediated responses, especially differences among ethnic groups.
- Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL.
- AhR gene expression associates with individual variation of CYP1A1 inducibility and CYP1B1 expression in cultivated lymphocytes
- Binding of ligands such as TCDD, beta-naphthoflavone, and benzopyrene metabolites to the Ah receptor is involved in human UGT1A1 induction.
- experiments revealed a complex distribution of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator mRNAs and proteins in rat and human testis
- DNA damage and cell cycle arrest induced by 2-(4-amino-3-methylphenyl)-5-fluorobenzothiazole (5F 203, NSC 703786) is attenuated in aryl hydrocarbon receptor deficient MCF-7 cells
- Inhibitory AhR-ERalpha cross talk is linked to a new pathway for degrading ERalpha in which TCDD initially induces formation of a nuclear AhR complex which coordinately recruits ERalpha & the proteasome complex, resulting in degradation of both receptors
- results confirm that the Ah receptor plays a critical role in B[a]P-7,8-dihydrodiol-induced apoptosis
- In mice transgenic to this receptor, there is a lessened susceptibility to dioxin-induced toxicity.
- estrogen receptor-mediated estrogen signalling is modulated by a co-regulatory-like function of activated AhR/Arnt, giving rise to adverse oestrogen-related actions of dioxin-type environmental contaminants
- the dioxin receptor is stabilized by XAP2
- Results describe the recruitment of aryl hydrocarbon receptor and associated proteins to the human cytochrome P4501A1 gene promoter in vivo.
- interactions between the aryl hydrocarbon receptor and estrogen receptor have a role in development of breast neoplasms [review]
- AhR tyrosine 9, which is not a phosphorylated residue itself but is required for DNA binding, appears to play a crucial role in AhR activity by permitting proper phosphorylation of the AhR.
- cell density regulates the intracellular localization and function of AhR, because of modulation of nuclear export activity
- Inhibitory AHR-androgen receptor crosstalk was studied in the LNCaP prostatic tumor cell line.
- AhR activation causes apoptosis and cell cycle arrest, especially through expression changes in genes related to apoptosis and cell cycle arrest.
- Results describe a mechanism whereby the aryl hydrocarbon receptor, a known transcriptional activator, mediates gene repression through interactions at E2F-responsive promoters, leading to the repression of E2F-dependent, S phase-specific genes.
- nucleotide preference of the heterodimers of AHR and Arnt
- Promoters and long-distance regulatory regions of AhR-responsive genes were analyzed by the genetic algorithm and a variety of other computational methods.
- Thirty-two single nucleotide variations have been identified in the aryl hydrocarbon receptor gene, including 25 novel ones and a GGGGC repeat polymorphism in the promoter region.
- MEK1 facilitates ligand-initiated transcriptional activation while targeting the Ah receptor for degradation
- Folding of the aryl hydrocarbon receptor transactivation domain modulates protein-protein interactions, such as the binding of transcription factor TATA-binding protein (TBP).
- AhR activation in the T-lineage cells is directly involved in thymocyte loss and skewed differentiation; AhR activation in T cells and not in B cells suppresses the immunization-induced increase in both T cells and B cells.
- ER alpha-AHR-ARNT protein-protein interactions mediate estradiol-dependent transrepression of dioxin-inducible gene transcription
- AHR pathway plays important role in cigarette smoke-mediated COX-2 and prostaglandin production in human lung fibroblasts and may contribute to tobacco-associated inflammation and lung disease.
- Induction of c-jun depends on activation of p38-mitogen-activated protein kinase (MAPK) by an AhR-dependent mechanism.
- Expression of BCRP is most likely aryl hydrocarbon receptor (AhR) dependent in Caco-2 cells.
- AHR-mediated transcription: ligand-dependent recruitment of Era to TCDD-responsive promoters was studied.
- AhR influences the expression of c-Myc, a protein critical to malignant transformation
- Results provide evidence for a cross-talk between CYP3A4 and aryl hydrocarbon receptor, and show that conclusions drawn from experiments carried out in cell lines may lead to erroneous in vivo predictions in man.
- This short review summarizes recent advances in the study of AhR activation and inducible expression of target gene cytochrome P450 (CYP1) and other CYP families
- enhanced AhR expression and especially its nuclear translocation may be a favorable factor for gastric cancer formation presumably via up-regulating CYP1A1 expression
- The increased AR gene activity observed in precious pubertyas indicated by the reduced AR gene methylation pattern, might lead to hypersensitivity of the hair follicles to steroid hormones and therefore to the premature development of pubic hair.
- The aryl hydrocarbon receptor activates the retinoic acid receptoralpha through SMRT antagonism
- findings show that Ahr activation is functionally connected to a signaling cascade leading to dramatic alterations of cell plasticity and increase in cell motility
- AhR-estrogen receptor 1/Sp1 transcription factor cross talk is due, in part, to enhanced association of AhR and estrogen receptor 1
- a functional interaction between NcoA4 and AhR that may alter AhR activity to affect disease development and progression is demonstrated
- The data presented support a major role for calpain in the AhR transformation, transactivation, and subsequent down-regulation, and provide a possible explanation for many of the reported phenomena of ligand-independent activation of AhR.
- This review discusses the role of the aryl hydrocarbon receptor, a ligand-activated transcription factor that regulates the transcription of a wide range of genes, including some drug metabolizing enzymes.
- Results suggest that the aryl hydrocarbon receptor participates in Slug induction, which, in turn, regulates cellular physiology including cell adhesion and migration.
- This study is the first to show an interaction between the CYP1A1 T3801C and AHR G1661A polymorphisms; this interaction could explain the difference in blood pressure level between smokers and non-smokers/ex-smokers
- A truncated Ah receptor blocks the hypoxia and estrogen receptor signaling pathways.
- AhR overexpression up-regulates the expression of CYP1B1 in the early stage of lung adenocarcinoma
- these observations support the hypothesis that there is a specific coordination between the activation of the cytosolic Ah receptor and the c-Src- and cdc37-containing HSP90 complex.
- investigated which roles the AR polymorphisms as well as the R554K and P185A in the AHR and AHRR genes, respectively, may play in modifying the effect of exposure to organohalogen pollutants in regard to sperm Y : X ratio
- Proliferation of breast cancer cells is accompanied not only by expression of genes encoding cytochromes involved in estrogen metabolism, but also by changes in the expression of other genes, such as AHR.
- findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex
- Taken together, these data establish beta7 ITG as a new molecular target of PAHs, whose up-regulation by these environmental contaminants most likely requires activation of co-operative pathways involving both AhR and c-maf.
- role of the AhR in the UVB stress response was confirmed in vivo by studies employing AhR KO mice
- AHR (aryl hydrocarbon receptor) agonists induce ERalpha promoter occupancy at AHR target genes through indirect activation of oestrogen receptor alpha(ERalpha), and support a role for ERalpha in AHR transactivation
- evidence presented for regulatory cross-talk between aryl hydrocarbon receptor and glucocorticoid receptor alpha in HepG2 cells.
- Allele and genotype frequencies of AHR and ARNT polymorphisms were similar between infertile men and controls.
- Role of AhR in drug metabolism demonstrates in vivo up-regulation of androstane receptor through chemical exposure.
- AhR activation and COX-2 overexpression likely represent a mechanism of resistance to apoptosis in lymphoma cell lines that might be relevant for the development of lymphoma in vivo.
- AhR with NF-kappaB RelB signaling pathways represent a new mechanism of cross talk between the two transcription factors
- The interaction of AhR with RelB binding on a novel type of NF-kappaB binding site represents a new regulatory function of the AhR.
- Hence the anti-inflammatory effect of PPARalpha overrides the pro-inflammatory effect of AhR.
- in response to PAHs, Ahr-mediated activation of the harakiri, BCL2 interacting protein (contains only BH3 domain), was necessary for execution of cell death.
- studies suggest that constitutively active AhR mediates different molecular outcomes than environmental chemical-activated AhR, and further implicate the AhR in mammary tumorigenesis
- Aryl hydrocarbon receptor mediates laminar fluid shear stress-induced CYP1A1 activation and cell cycle arrest in vascular endothelial cells.
- AhR, a transcription factor, can bind specifically to AD1 in the C-terminal region of BRCA1 and affect BRCA1's ability to regulate transcription activity.
- These findings indicate that the cross-talk function of the AhR with estrogen or androgen receptors is an intrinsic function of the AhR.
- Reduction in VEGF correlates with inhibited prostate carcinogenesis in Ahr transsgenic mice.
- AhR-mediated regulation of the human UGT1A4 gene by two xenobiotic response elements and a modulation by single nucleotide polymorphisms is demonstrated
- analysis of how inflammatory signaling and aryl hydrocarbon receptor mediate synergistic induction of interleukin 6 in MCF-7 cells
- After high glucose stimulation, AhR was found in complex with Egr-1 & activator protein-2. The activity of the DNA-binding complex was regulated by glucose (a physiological stimulus) via activation of hexosamine pathway & intracellular glycosylation.
- AHR binds to E2F1 and inhibits E2F1-induced apoptosis
- polymorphisms of AhR gene were associated with the level of 1-OHP among PAH-exposed workers, suggesting that AhR-mediated signaling might contribute to individual susceptibility to PAH exposure.
- These data demonstrate xenobiotic induced regulation of the UGT1A3 gene by the aryl hydrocarbon receptor, which shows genetic variability
- CyP40 is found in the cell nucleus after 3-methylchloranthrene treatment and appears to promote the dioxin response element binding form of the AhR/Arnt heterodimer.
- AhR is a master regulator of c-raf and propose cross-talk between AhR and the mitogen-activated protein kinase signaling pathway.
- AHR polymorphisms and potential gene-smoking interaction may be involved in the etiology of lung cancer.
- Prunella vulgaris extract activities were probably related to an ability to function as an aryl hydrocarbon receptor (AHR) agonist in ECC-1 cells.
- broccoli-derived SUL can directly induce Cyp1a1 gene expression in an AhR-dependent manner and represents a novel mechanism by which sulforaphane induces this enzyme
- Curcumin attenuates cytochrome P450 induction in response to TCDD by ROS-dependently degrading AhR and ARNT.
- AhR activation plays an essential part in the development of Th17 cells.
- Heat shock protein 90 inhibitors suppress aryl hydrocarbon receptor-mediated activation of CYP1A1 and CYP1B1 transcription and DNA adduct formation.