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Validated All-in-One™ qPCR Primer for PRMT1(NM_001207042.2) Search again
Product ID:
HQP054510
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ANM1, HCP1, HRMT1L2, IR1B4
Gene Description:
protein arginine methyltransferase 1
Target Gene Accession:
NM_001207042.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).[supplied by OMIM].
Gene References into function
- several proteins extracted from HeLa cells were methylated by PRMT1
- SAF-A is arginine methylated by PRMT1
- These results suggest that PRMT1 plays a post-translationally important role in regulating the transcriptional activity.
- PRMT1 arginine-methylates MRE11 and thus regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response.
- A fraction of PRMT1 is located in the nucleus, but the protein is predominantly cytoplasmic.
- GAR (Glycine Arginine rich) motif is a region required for 53BP1 DNA binding activity and as the site of methylation by PRMT1.
- a glycine-arginine rich (GAR) stretch of 53BP1 lying upstream of the Tudor motifs is methylated by PRMT1
- arginine dimethylation of heterogeneous nuclear ribonucleoprotein K by protein-arginine methyltransferase 1 inhibits its interaction with c-Src
- SET-mediated promoter hypoacetylation is a prerequisite for coactivation of the estrogen-responsive pS2 gene by PRMT1
- Methylates Ki-1/57 and CGI-55 (PAI-1 mRNA-binding protein) at a conserved Gly/Arg-rich motif cluster.
- PRMT1 is a coactivator for HNF4, an orphan nuclear receptor that regulates the expression of genes involved in diverse metabolic pathways
- CAF1 is a new regulator of PRMT1-dependent arginine methylation.
- Our results suggest that PRMT1 might be involved in the previously reported methylation of Arg25 in HMGA1a in vivo.
- relative balance of PRMT1 isoforms is altered in breast cancer
- Study uncovers an essential function of PRMTs in oncogenesis and reveals their potential as novel therapeutic targets in human cancer.
- Our results suggest that PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3-K pathway and subsequent glucose uptake in skeletal muscle cells.
- PRMT1- dependent methylation of RUNX1 at arginine residues 206 and 210 abrogates its association with SIN3A.
- C-terminal domain containing the methylated arginine residues is known to promote PAPBN1 self-association, and arginine methylation has been reported to inhibit self-association of an orthologous protein
- These data indicate that the methylation of estrogen receptor alpha is a physiological process occurring in the cytoplasm of normal and malignant epithelial breast cells and that ERalpha is hypermethylated in a subset of breast cancers.
- PRMT1 selectively recognizes a set of amino acid sequences in substrates that extend beyond the "RGG" paradigm.
- examined the expression pattern of protein arginine methyltransferase 1 gene in colon cancer patients
- findings identify PRMT1 as a novel and crucial negative regulator of STAT1 activation that controls PIAS1-mediated repression by arginine methylation