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Validated All-in-One™ qPCR Primer for YWHAZ(NM_001135702.1) Search again
Product ID:
HQP054302
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
14-3-3-zeta, HEL-S-3, HEL-S-93, HEL4, KCIP-1, POPCHAS, YWHAD
Gene Description:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
Target Gene Accession:
NM_001135702.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq].
Gene References into function
- Alpha-synuclein, a small presynaptic protein implicated in Parkinson's disease and other neurodegenerative disorders, interacts with 14-3-3 and both proteins appear to differentially regulate the activities of important cellular enzymes.
- Identification of 14-3-3zeta as a protein kinase B/Akt substrate.
- 14-3-3 zeta binds with tuberin to regulate phosphorylation of ribosomal protein S6
- binds cofilin and LIM-domain-containing protein kinase 1
- TSC2 associates with 14-3-3 in vivo
- role for the 14-3-3zeta/ADAM 22 association in the regulation of cell adhesion and related signaling events
- 14-3-3zeta has a role in MAPKAPK2-mediated phosphorylation, which may represent a novel pathway mediating p38 MAPK-dependent inflammation
- In the presence of 14-3-3, the requirement of two phosphorylated 14-3-3 zeta binding motifs for the complete inhibition of FOXO4 binding to its target DNA is demonstrated.
- Zeta 14-3-3 protein facilitates tau assembly into fibrillar polymers, with polymerization reduced but not abolished, when tau is modified by protein kinase A.
- 14-3-3zeta is a specific substrate for YSK1 that localizes to the Golgi apparatus, and potentially links YSK1 signaling at the Golgi apparatus with protein transport.
- existence of a second major 14-3-3zeta binding site within the cytoplasmic tail of GPIbalpha that has an important functional role in regulating integrin-dependent cell spreading.
- within the tau phosphorylation complex, 14-3-3 connects Ser(9)-phosphorylated GSK3beta to tau and Ser(9)-phosphorylated GSK3beta phosphorylates tau
- EGF-induced co-localization of mouse EGFR and human 14-3-3zeta along the plasma membrane.
- a 14-3-3 zeta implication in the Alzheimer's neuropathology
- FRET analysis of 14-3-3 protein C-terminal stretch
- YWHAZ gene is a susceptibility gene for paranoid schizophrenia
- Vpr interferes with the suppressive effects of insulin on FOXO-mediated transcription of target genes via 14-3-3. (14-3-3 Protein)
- binding of 14-3-3 to Par3beta is dependent on phosphorylation
- 14-3-3 binds to protein C-terminal domains
- Our data demonstrate that 14-3-3zeta mediates tau phosphorylation by Ser9-phosphorylated GSK3beta in HEK-293 cells.
- Knockdown of 14-3-3 zeta induces at least two isoform-specific phenotypes that are consistent with potential oncogenic activity during tumorigenesis.
- findings suggest that over-expression of 14-3-3zeta is an early event in oral tumorigenesis and may have an important role in its development and progression
- 14-3-3zeta is a granule protein related to atherosclerosis
- Our data support a novel role for 14-3-3zeta in the aggregate formation of nonnative, aggregation-prone proteins.
- 14-3-3zeta has a critical role in anoikis suppression in lung cancer cells
- 14-3-3 zeta protein and butyrylcholinesterase (BCHE) synergistic effects between polymorphisms in tau phosphorylation relate-genes may help in determining the risk profile for AD.
- combined gene effects between 14-3-3 zeta (intron 4, rs 983583) polymorphism and tau (intron 9, rs 2471738) polymorphism might be responsible for susceptibility to Alzheimer disease
- PKC delta in preeclamptic placentas promotes Bax dissociation from 14-3-3 zeta through 14-3-3 zeta phosphorylation.
- structure & properties of wild type 14-3-3zeta & point mutants S58E, S184E, T232E & mutant S58E/S184E/T232E mimicking phosphorylation; mutation mimicking phosphorylation of Ser58 destabilized & mutation of Ser184 induced stabilization of 14-3-3zeta
- 14-3-3epsilon and 14-3-3zeta are identified as Cby-binding partners.
- Immunofluorescent staining for 14-3-3 zeta demonstrated expression of the protein on ascitic and peritumoral macrophages in epithelial ovarian carcinoma patients
- This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia.
- Phosphorylation by cAMP-dependent protein kinase strongly enhanced interaction of tau3 with 14-3-3.
- The authors report that phosphorylation leads to the unfolding of the structurally atypical and unstable KH1, creating a site for 14-3-3zeta binding.