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Validated All-in-One™ qPCR Primer for FUS(NM_004960.3) Search again
Product ID:
HQP054264
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS
Gene Description:
FUS RNA binding protein
Target Gene Accession:
NM_004960.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Temperature-dependent localization of TLS-CHOP to splicing factor compartments and association with Cajal bodies. A region within the N-terminal sequence required for colocalization with the splicing factor SC-35 is identified.
- Proto-oncoprotein TLS/FUS is associated to the nuclear matrix and complexed with splicing factors PTB, SRm160, and SR proteins and plays a role in spliceosome assembly
- FUS was fused with BBF2H7 in a low grade myxoid liposarcoma.
- data suggest that different FUS/CHOP variants cause transformation of mesenchymal cells via the same pathways with comparable efficacy
- the zinc finger domain plays a more predominant role in RNA recognition than the RRM domain in TLS
- Effect of FUS-DDIT3 fusion on IL6 expression is C/EBP beta dependent in myxoid lipoxarcoma.
- The oncogenic TLS-ERG fusion protein activates two different sets of genes sharing little similarity when transforms hematopoietic cells and fibroblasts.
- Patients with immunophenotype of Pre-B-acute lymphoblastic leukemia were found to carry: TLS/ERG.
- Interaction of beta-catenin with FUS/TLS and other RNA-binding proteins involved in the regulation of pre-mRNA splicing in colorectal cancer
- Development of lipoblasts and the typical MLS/RCLS capillary network could be an effect of the DDIT3 transcription factor partner of the fusion oncogene.
- FUS-DDIT3 is a chimeric oncogene generated by the most common chromosomal translocation t(12;16)(q13;p11) associated to liposarcomas.
- Presence of FUS/CREB3L2 and FUS/CREB3L1 in low-grade fibromyxoid sarcoma and sclerosing epithelioid fibrosarcoma suggests these neoplasms may be related.
- the sequestration of TLS to polyQ aggregates may play a role in diverse pathological changes in the brains of patients with polyQ diseases.
- TLS serves as a key transcriptional regulatory sensor of DNA damage signals that, on the basis of its allosteric modulation by RNA, specifically binds to and inhibits CBP and p300 HAT activities on a repressed gene target, cyclin D1
- FUS, EWS and TAF15 proto-oncoproteins were targeted to stress granules induced by heat shock and oxidative stress
- IGF1 is a common target gene of Ewing's sarcoma fusion proteins EWS-FLI-1, EWS-ERG and FUS-ERG in mesenchymal progenitor cells
- CHOP is a member of the transcription factor network that controls the stress-induced regulation of specific C/EBP-ATF4-containing genes, such as ASNS
- All cases of pleomorphic liposarcoma (PLS) showed a varied distribution of extra signals with polyploidy and amplification in each histological area. No CHOP fusion transcript was found in any case of PLS by nested RT-PCR.
- study reports 13 mutations in FUS/TLS gene on chromosome 16 that were specific for familial amyotrophic lateral sclerosis; mutant forms of FUS/TLS accumulated in the cytoplasm of neurons
- identification of missense mutations in FUS in familial amyotrophic lateral sclerosis type 6; expression studies revealed aberrant localization of mutant FUS protein