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Validated All-in-One™ qPCR Primer for LPAR1(NM_057159.3) Search again
Product ID:
HQP054028
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EDG2, Gpcr26, LPA1, Mrec1.3, VZG1, edg-2, rec.1.3, vzg-1
Gene Description:
lysophosphatidic acid receptor 1
Target Gene Accession:
NM_057159.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq].
Gene References into function
- LP(A1) and myelin basic protein colocalized in brain, but oligodendrocyte soma showed stronger signals for LP(A1) than myelinated fibers, whereas the reverse was true for myelin basic protein so LP(A1) may be involved in myelin formation or maintenance.
- demonstrate that two biological fluids, blood plasma and seminal plasma, differentially activate LPA receptors
- Data suggest that LPA(1) receptors couple to a G(i)-phosphoinositide 3-kinase-Tiam1 pathway to activate Rac, with consequent suppression of RhoA activity, and thereby stimulate cell spreading and motility.
- lysophosphatidic acid-coupled LPA1/EDG-2 receptors are endocytosed via a dynamin2- and Rab5-dependent pathway
- LPA acts as a potent stimulator of colon cancer progression, although the binding to LPA1 and LPA2 induces slightly different responses.
- LPA stimulation promotes the interaction of the LPA(2) receptor with a focal adhesion molecule, TRIP6
- EDG-2 expression was increased in low-grade adenoma compared with that in normal mucosa (P < 0.001). EDG-2 expression was significantly greater in adenomas with larger diameters (P < 0.001).
- Amyloid beta-protein stimulated in monocytes the gene expression for sphingosine-1-phosphate receptor 2, which is amyloid beta-protein-induced migration.
- data suggest that endothelial differentiation gene EDG-7 and EDG-2 lysophosphatidic acid receptors play a diverse role in mesangial cell proliferation
- formation of the LPA receptor/PDZ domain-containing RhoGEF complex plays a pivotal role in LPA-induced RhoA activation
- We show that in addition to promoting LPA(1) signaling, membrane cholesterol is essential for the association of LPA(1) with beta-arrestin, which leads to signal attenuation and clathrin-dependent endocytosis of LPA(1).
- These findings demonstrate that trafficking of LPA1 to the nucleus is influenced by cell-matrix interactions and that nuclear LPA1 may be involved in regulating intranuclear protein phosphorylation and signalling.
- LPA(1) transduces Galphai-dependent signals to promote nuclear localization of androgen receptor and cell proliferation
- EDG2 and EDG4 cooperate to promote LPA-stimulated chemotaxis in breast tumor cell lines.
- The results of the present study suggest that lysophosphatidic acid (LPA), the receptor LPA(1), ERK2 and p38alpha are important regulators for prostate cancer cell invasion and thus could play a significant role in the development of metastasis.
- expression of LPA-induced inflammatory response genes is mediated by LPA1 and LPA3
- Lysophosphatidic acid receptor 1 may contribute to the pathogenesis of rheumatoid arthritis through the modulation of fibroblast-like synoviocyte migration and cytokine production.
- distinct molecular mechanisms regulate agonist-dependent and PMA-dependent internalization of the LPA 1 receptor.
- Down-regulation of EDG2 is functionally important to suppression of tumor metastasis in breast neoplasms.
- Through the stepwise association study, an SNP located in the promoter region of EDG2 (-2,820G/A; rs10980705) showed significant association with knee osteoarthritis in two independent populations.
- LPA may play a role in angiogenesis of endometrium and placenta through induction of IL-8 in endometrial stromal cells during pregnancy.
- Lysophosphatidic acid might regulate VEGF-C and lymphatic marker expression in endothelial cells, which contributes to endothelial cell tube formation in vitro and in vivo, thus facilitating endothelial cell participation lymphangiogenesis.
- A role for the transgenic lysophosphatidic acid (LPA)1 receptor is identified in regulating smooth muscle cell migratory responses in the context of vascular injury.
- These results indicate that LPA is a critical factor on proliferation though LPA(1), and on motility though LPA(2) in MPM cells.
- Switching of LPA receptor expression from LPA3 to LPA1, may be involved in prostate cancer progression and/or androgen independence