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Validated All-in-One™ qPCR Primer for ZAP70(NM_001079.3) Search again
Product ID:
HQP053905
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70
Gene Description:
zeta chain of T cell receptor associated protein kinase 70
Target Gene Accession:
NM_001079.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- association of Zap-70 induced by T cell activation in plasma membrane microdomains with GM3
- Signaling through ZAP-70 is required for CXCL12-mediated T-cell transendothelial migration.
- ZAP-70, a tyrosine kinase known to be crucial for T cell activation, as a key player in TCR down-modulation and zeta degradation.
- ZAP-70 signaling drives the TCR-dependent reorientation of the microtubule-organizing center
- Expression of ZAP-70 is associated with enhanced signal transduction via the BCR complex, which may contribute to the more aggressive clinical course associated with CLL cells that express nonmutated immunoglobulin receptors.
- VHR, a Vaccinia virus VH1-related dual-specific protein phosphatase, is phosphorylated at Y138 by ZAP-70.
- profiling of gene expression in purified chronic lymphocytic leukemia(CLL)samples from 107 patients showed that ZAP-70 expression is the best discriminator of Ig-mutated and Ig-unmutated CLL
- Impaired ZAP-70 activation is associated with a unique signaling pathway coupling TCR ligation with T cell proliferation.
- ZAP-70 is required for intracellular calcium ion mobilization in response to SDF-1alpha/CXCL12-induced prolonged activation of extracellular signal-related protein kinase in Jurkat T lymphocytes.
- The requirement for ZAP-70 is clearly demonstrated in T cell receptor-induced polarization of the T cell's microtubule-organizing center, as it moves toward the interface of the T cell and antigen-presenting cell.
- concluded that phosphorylation of ZAP-70 at Ser-520 plays an important role in the correct localization of ZAP-70 and in priming ZAP-70 for its acute recruitment and activation upon antigen receptor ligation
- Lck and ZAP-70 have roles in the interaction of human MUC1 and beta-catenin in activated Jurkat T cells
- The interplay between Syk and ZAP-70 in thymocytes, certain T cells, and B-chronic lymphocytic leukemia cells will modulate the amplitude of antigen-mediated receptor signaling.
- The interdomain B region of ZAP-70 regulates beta 1 integrin activation by the CD3/T cell receptor complex via control of tyrosine phosphorylation of tyrosine residues 171 and 191 in the linker for activation of T cells (LAT) cytoplasmic domain.
- ZAP-70 is expressed by many lymphoma types, correlates with immunoglobulin heavy-chain variable region gene mutational status and can be detected reliably using immunohistochemical methods.
- Essential for T cell receptor signaling and CD8 cell selection.
- three-dimensional structure of the ZAP-70 kinase domain in complex with staurosporine
- PD-1 modulation of T-cell function involves inhibition of TCR-mediated phosphorylation of ZAP70 and association with CD3zeta
- ZAP-70 is associated with the mutational status of Ig VH genes in B-CLLs; high levels of ZAP-70 correlated with Binet stages B or C indicating an involvement of ZAP-70 in mechanisms promoting growth of B-CLL cells.
- expression of ZAP-70 in CLL allows for more effective IgM signaling in CLL B cells, a feature that could contribute to the relatively aggressive clinical behavior generally associated with CLL cells that express unmutated IgV(H)
- The ZAP-70 expression analyses provided complementary prognostic information identifying three patient subgroups with good, intermediate and poor prognosis.
- ZAP-70 is expressed in a subpopulation of tonsillar and splenic normal B-lymphocytes that express an activated phenotype
- murine leukemia model with many similarities to human ZAP-70+ B cell chronic lymphocytic leukemia when human B cells are transplanted into NOD/SCID mice.
- To analyze the expression of Syk tyrosine kinase during the multi step development of human breast carcinoma
- These findings suggest that a general autoinhibitory mechanism employed by RTKs is also used by some cytoplasmic tyrosine kinases, such as ZAP-70.
- ZAP-70 is required for cell survival and signaling in chronic lymphocytic leukemia, and inhibition of Hsp90 leads to ZAP-70 degradation, apoptosis, and impaired signaling
- ZAP-70 is consistently expressed and phosphorylated in Blin-ALL cells
- oorellation between expression of ZAP-70 and methylation status of a specific CpG site at the intron 1 - exon 2 boundary of the ZAP-70 gene in chronic lymphocytic leukemia.
- Methylation of a highly conserved intronic region of ZAP-70 may be responsible for regulation of expression in normal and chronic lymphocytic leukemia cells.
- results suggest a novel functional role for ZAP 70 in nuclear receptor-driven transactivation in T lymphocytes.
- The difference in the spatio-temporal localization of LCK and ZAP70 proteins following stimulation may eliminate signal crosstalk, and could explain the differentiation of the specific downstream responses of these pathways
- May be a prognostic marker in chronic lymphocytic leukemia. (review)
- In a variety of cell types, Syk is the tyrosine kinase that plays an important role in the activation of Tpl2.
- The aberrant expression of ZAP-70 in more aggressive forms of chronic lymphocytic leukemia requires the chaperoning action of activated heat-shock protein 90, which may be specifically inhibited by the therapeutic strategies discussed in this review.
- results suggest that biological functions attributed to the association of Zap70 with Vav after T cell activation may equally reflect the association of Zap70 with CrkII, and further support a regulatory role for CrkII in TCR-linked signal transduction
- Dexamethasone induces rapid tyrosine-phosphorylation of ZAP-70 in Jurkat cells
- The ZAP-70-TCR-zeta association mediates the activation of PLC-gamma1 leading to T cell responses even in the absence of kinase activation of ZAP-70.
- There were characteristic modes of discordance between ZAP-70 and VH mutation status depending on the presence or absence of additional genetic high-risk features such as 11q and 17p deletion or V3-21 usage.
- The ZAP70 protein was found expressed in normal pro/pre B cells but not in a significant proportion of normal B cells with mature phenotype. The presence of ZAP-70 in B-ALLs probably reflects their cellular origin.
- Higher cyclin E expression in samples of ZAP 70-positive patients may reflect a larger proliferating compartment in vivo compared to ZAP 70-negative patients with B-cell chronic leukemia.
- B-prolymphocytic leukemia appears biologically heterogeneous regarding IgVH mutations, ZAP-70 and CD38 expression, showing a pattern distinct from that of other lymphoproliferative disorders
- The ZAP 70 was expressed by 91% of the tumor cells within the bone marrow. The Variability of ZAP 70 expression is possible associated with disease prognosis in a patient in CLL.
- IRTA4/FcRH2 expression as detected by flow cytometry was significantly lower in the poor prognosis subgroup as compared to ZAP-70-CD38- B-chronic lymphocytic leukemia cells.
- role of ZAP-70 in B-cell receptor signaling is quite distinct from its role in T-cell receptor signaling
- ZAP-70 reactivity using a T-cell marker as a control allows to identify the majority of patients with an unmutated Ig VH genotype.
- ZAP-70 kinase regulates HIV cell-to-cell spread and virological synapse formation.
- These findings raise the possibility that ZAP-70 may serve as an important link between GC and TcR-induced signaling, thereby transmitting non-genomic GC action in T-cells.
- Both the zeta chain and the Zap 70 expression in circulating T lymphocytes are down-regulated in patients with laryngeal cancer and that these changes do not immediately return to normal after surgery.
- LAT, ZAP-70, and DNMT1 protein levels in CD4(+) T cells can be associated with systemic lupus erythematosus.
- These interactions are inconsistent with ITAM binding, suggesting that destabilization of this autoinhibited ZAP-70 conformation is the first step in kinase activation
- Prognostic significance of combined analysis of ZAP-70 and CD38 in chronic lymphocytic leukemia was performed.
- ZAP-70 expression over time in B chronic lymphocytic leukaemia.
- ZAP70 mRNA quantification via qPCR is a strong surrogate marker of IGHV mutational status and a powerful prognostic factor.
- The point mutation found in Zap70 was causative for the DeltaT3 phenotype of primary celluluar immunodefiencies, including increased IgE plasma levels, a phenotype that has not been associated with altered Zap70 function in the past.
- Zap70 signaling plays an important role in dexamethasone receptor-mediated transactivation of T lymphocytes.
- ZAP70 is expressed in a higher percentage of tumor cells in the aggressive non-germinal center subgroup of diffuse large B-cell lymphoma.
- Results suggest that Zap-70 expression may be a better indicator of the mutational status of IgVH and prognosis of chronic lymphocytic leukemia than CD38 expression.
- The efficiency and reliability of flow cytometric detection of ZAP-70 can be optimized by using Scatchard analysis to help select the most effective antibodies and antibody concentrations that maximize specific to nonspecific binding.
- in Jurkat cells, ZAP-70 phosphorylation level increased under acidic environments; results suggest that extracellular low pH stimulates or enhances TCR signaling via ZAP-70 and p38
- Immune synapse formation requires ZAP70 recruitment by ezrin and CD43 removal by moesin.
- ZAP-70 most likely acts as an adapter protein that facilitates B-cell receptor (BCR) signaling in CLL cells independent of its tyrosine kinase activity or its ability to interact with c-Cbl.
- presents three CLL patients with discordant ZAP80 levels
- absence of IgV(H) mutational status and ZAP-70-positivity were associated with a shorter time to first treatment; ZAP-70 did not correlate with prognostic variables
- Results highlight the importance of a high intracellular Zn(2+) content and the VHR/ZAP-70/ERK1,2-associated pathways in the modulation of LNCaP prostate cancer cell growth.
- Regardless of CD38 expression level, CD38 and ZAP-70 expressions were significantly associated in B-CLL
- The T cell-associated protein kinase ZAP-70 is highly expressed in pre-B lineage cells and most cases of pre-B acute lymphoblastic leukemia. ZAP-70 expression may hold prognostic value for pre-B ALL and raises the prospect of a novel therapeutic target.
- CEACAM1 orchestrate an inhibitory program that abrogates extremely proximal events downstream of the TCR/CD3 complex by focusing on the activation of ZAP-70.
- There was a significant correlation between the percentage of leukemic cells positive for ZAP-70 and the percentage of CD38+CD19+ cells (R=0.629; p=0.000001).
- ZAP-70 is the risk factor for disease progression in chronic lymphocytic leukemia
- ZAP-70 activity is inhibited specifically by EGCG, which contributed to suppressing the CD3-mediated T cell-induced pathways in leukemia cells
- positive ZAP-70 and CD38 status were associated with an unfavorable clinical course in chronic lymphocytic leukemia patients
- determination of ZAP-70 in B cells by RTqPCR has an excellent correlation with IGHV mutational status
- Flow cytometry presents advantages over other methods to detect ZAP-70, and its quantification has proved its predictive value in chronic lymphocytic leukemia.
- A method to index ZAP-70 protein expression levels to internal positive and negative cell populations with chronic lymphocytic leukemia.
- Syk functions as an "OR-gate" type of molecular switch and is more readily activated by autophosphorylation than Zap-70
- review of current knowledge concerning the biological feature and function of TCR zeta protein, splice variant and mutation of TCR zeta chain gene and alteration of expression pattern in hematological diseases
- while Vav-1 and LAT preferentially bound to Syk, phospholipase C-gamma1 bound to both Syk and ZAP-70
- Simultaneous analysis of ZAP-70, CD38 and IGHV mutations in CLL provides more discriminatory prediction of treatment-free interval than any factor alone
- ZAP70 expression in human B cells represents an exclusive hallmark of Toll-like receptor (TLR)9-mediated B cell activation.