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Validated All-in-One™ qPCR Primer for MED12(NM_005120.2) Search again
Product ID:
HQP023419
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ARC240, CAGH45, FGS1, HDKR, HOPA, Kto, MED12S, OHDOX, OKS, OPA1, TNRC11, TRAP230
Gene Description:
mediator complex subunit 12
Target Gene Accession:
NM_005120.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- It is unlikely that the 12 bp duplication variant has relevance to the susceptibility to different subtypes of autism.
- The TRAP230 subunit of TRAP/Mediator was shown to interact directly with RTA.
- The HOPA12bp polymorphism is a significant risk factor for schizophrenia for both men and women.
- the MED12 interface within Mediator is a new component in the Wnt/beta-catenin pathway
- reveal a regulatory role of motionless/Med12 in vertebrate neuronal development
- the HOPA(12bp) allele is a risk factor for schizophrenia in subjects of European ancestry
- Recurrent mutation (2881C>T, leading to R961W) in MED12 located at Xq13, linked to Opitz-Kaveggia syndrome.
- The original Lujan syndrome family has a novel missense mutation (p.N1007S) in the MED12 gene.
- Review concludes that a thorough understanding of MED12's role in transcriptional regulation could have significant benefits for human healthcare.
- These findings implicate Mediator in epigenetic restriction of neuronal gene expression to the nervous system and suggest a pathologic basis for MED12-associated X-linked mental retardation involving impaired REST-dependent neuronal gene regulation.
- Males with this MED12 mutation had deficits in communication skills compared to their socialization and daily living skills.
- Med12--but not Med13--is essential for activating the CDK8 kinase.
- Med12 and Med13 are critical for subcomplex-dependent repression, whereas CDK8 kinase activity is not